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Study on the structure and functions of sulfated glycosaminoglycans involved in the development or treatment of liver diseases.

Research Project

Project/Area Number 19590052
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionHokkaido University

Principal Investigator

YAMADA Shuhei  Hokkaido University, 大学院・先端生命科学研究院, 准教授 (70240017)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsグリコサミノグリカン / コンドロイチン硫酸 / ヘパラン硫酸 / 肝再生 / C型肝炎ウイルス / 細胞増殖因子 / サイトカイン / C型肝炎ウィルス
Research Abstract

硫酸化グリコサミノグリカン(GAG)は細胞増殖因子など様々な生理活性タンパク質と特異的に相互作用し、それらの活性を調節する。また、ウィルス感染の過程においても極めて重要な役割を果たしている。本研究では、再生期間の異なる再生肝よりGAG を精製し、その構造変化と機能変化を解析した。また、様々なGAG について、C 型肝炎ウィルスの感染への影響を検討した。さらにGAG の構造解析を行うための新規の技術や酵素を開発した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (49 results)

All 2009 2008 2007 2004

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (28 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Isolation and Characterization of a Novel Chondroitin Sulfate from Squid Liver Integument Rich in N-Acetylgalactosamine(4,6-O-disulfate) and Glucuronate(3-O-sulfate) Residues2009

    • Author(s)
      Ajaya Kumar Shetty, Takanari Kobayashi, Shuji Mizumoto, Masaki Narumi, Yoshiaki Kudo, Shuhei Yamada, and Kazuyuki Sugahara
    • Journal Title

      Carbohydr. Res. (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural and Functional Changes of Sulfated Glycosaminoglycans in Xenopus laevis during Embryogenesis2009

    • Author(s)
      Shuhei Yamada, Masako Onishi, Reiko Fujinawa, Yuko Tadokoro, Koji Okabayashi, Makoto Asashima, and Kazuyuki Sugahara,
    • Journal Title

      Glycobiology (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural and Functional Changes of Sulfated Glycosaminoglycans in Xenopus laevis during Embryogenesis2009

    • Author(s)
      Shuhei Yamada
    • Journal Title

      Glycobiology (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetvlaalactosamine (4.6-O-disulfate) and alucuronate(3-O-sulfatel) residues2009

    • Author(s)
      Ajaya Kumar Shetty
    • Journal Title

      Carbohydrate Research (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identificationof a Novel Chondroitin Hydrolase inCaenorhabditis elegans. These authorscontributed equally to the work2008

    • Author(s)
      Tomoyuki Kaneiwa, Shuhei Yamada, Shuji Mizumoto, Adriana M. Montano, Souhei Mitani and Kazuyuki Sugahara
    • Journal Title

      J. Biol. Chem. 283

      Pages: 14971-14979

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential Therapeutic Application ofChondroitin Sulfate/Dermatan Sulfate2008

    • Author(s)
      Shuhei Yamada and Kazuyuki Sugahara
    • Journal Title

      Current Drug Discovery Technologies 5

      Pages: 289-301

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination ofIduronic Acid and Glucuronic Acid in SulfatedChondroitin/Dermatan Hybrid Chains by1H-Nuclear Magnetic Resonance Spectroscopy.*These authors contributed equally to the work.2008

    • Author(s)
      Fuchuan Li, Shuhei Yamada, Basappa, Ajaya K. Shetty, Makiko Sugiura, and Kazuyuki Sugahara
    • Journal Title

      Glycoconjugate J. 25

      Pages: 603-610

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of Iduronic Acid and Glucuronic Acid in Sulfated Chondroitin/Dermatan Hybrid Chains by ^1H-Nuclear Magnetic Resonance Spectroscopy2008

    • Author(s)
      Fuchuan Li
    • Journal Title

      Glycoconjugate J. 25

      Pages: 603-610

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential Therapeutic Application of Chondroitin Sulfate/Dermatan Sulfate2008

    • Author(s)
      Shuhei Yamada
    • Journal Title

      Current Drug Discovery Technologies 5

      Pages: 289-301

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a Novel Chondroitin Hydrolase in Caenorhabditis elegans2008

    • Author(s)
      Tomoyuki Kaneiwa
    • Journal Title

      J. Biol. Chem. 283

      Pages: 14971-14979

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of Iduronic Acid and Glucuronic Acid in Sulfated Chondroitin/Dermatan Hybrid Chains by 1H-Nuclear Magnetic Resonance Spectroscopy2008

    • Author(s)
      Fuchuan Li
    • Journal Title

      Glycoconjugate J. (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chondroitinase-mediatedDegradation of Rare 3-O-Sulfated GlucuronicAcid in Functional Oversulfated Chondroitin Sulfate K and E.2007

    • Author(s)
      Duriya Fongmoon, Ajaya Kumar Shetty, Basappa, Shuhei Yamada, Makiko Sugiura,Prachya Kongtawelert, and Kazuyuki Sugahara
    • Journal Title

      J. Biol. Chem. 282

      Pages: 36895-36904

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glycosaminoglycans in Hydra magnipapillata(Hydrozoa, Cnidaria) : Demonstration ofChondroitin in the Developing Nematocyst,Sting Organelle, and Structural Characterization of Glycosaminoglycans2007

    • Author(s)
      Shuhei Yamada, Hideto Morimoto, ToshitakaFujisawa, and Kazuyuki Sugahara
    • Journal Title

      Glycobiology 17

      Pages: 886-894

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] StructuralDetermination of Novel SulfatedOctasaccharides Isolated from ChondroitinSulfate of Shark Cartilage and TheirApplication for Characterizing MonoclonalAntibody Epitopes.2007

    • Author(s)
      Sarama S. Deepa, Shuhei Yamada, Shigeyuki Fukui, and Kazuyuki Sugahara
    • Journal Title

      Glycobiology 17

      Pages: 631-645

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural determination of novel sulfated octasaccharides isolated from chondroitin sulfate of shark cartilage and their application for characterizing monoclonal antibody epitopes.2007

    • Author(s)
      Sarama S. Deepa
    • Journal Title

      Glycobiology 17

      Pages: 631-645

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glycosaminoglycans in Hydra magnipapillata(Hydrozoa, Cnidaria): demonstration of chondroitin in the developing nematocyst, sting organelle, and structural characterization of glycosaminoglycans.2007

    • Author(s)
      Shuhei Yamada
    • Journal Title

      Glycobiology 17

      Pages: 886-894

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chondroitinase-mediated Degradation of Rare 3-Ο-Sulfated Glucuronic Acid in Functional Oversulfated Chondroitin Sulfate K and E.2007

    • Author(s)
      Duriya Fongmoon
    • Journal Title

      J. Biol. Chem. 282

      Pages: 36895-36904

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Structural analysis ofchondroitin sulfate and dermatansulfate in heparan sulfate glucuronylC5-epimerase-deficient mice、BMB20082008

    • Author(s)
      山田修平
    • Organizer
      第81回日本生化学会大会・第31回日本分子生物学会年会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Final Research Report
  • [Presentation] 硫酸化グリコサミノグリカンとC型肝炎ウイルスのエンベロープタンパク質との相互作用の解析2008

    • Author(s)
      小林芙美
    • Organizer
      BMB2008(第81回日本生化学会大会・第31回日本分子生物学会年会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Final Research Report
  • [Presentation] 硫酸化グリコサミノグリカンとC型肝炎ウイルスのエンベロープタンパク質との相互作用の解析2008

    • Author(s)
      小林芙美
    • Organizer
      BMB2008(第81回日本生化学会大会・第31回日本分子生物学会年会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Structural analysis of chondroitin sulfate and dermatan sulfate in henaran sulfate glucuronyl C5-enimerase-deficient mice2008

    • Author(s)
      山田修平
    • Organizer
      BMB2008(第81回日本生化学会大会・第31回日本分子生物学会年会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] C型肝炎ウイルスのエンベロープタンパク質と硫酸化グリコサミノグリカンとの相互作用解析2008

    • Author(s)
      小林芙美
    • Organizer
      シオノギイノベーションフェアー2008
    • Place of Presentation
      札幌
    • Year and Date
      2008-08-28
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] C型肝炎ウイルスのエンベロープタンパク質と硫酸化グリコサミノグリカンとの相互作用解析2008

    • Author(s)
      小林芙美
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      2008-08-20
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 線虫Caenorhabditis elegansのコンドロイチン加水分解酵素による主要な分解産物の構造解析2008

    • Author(s)
      金岩知之
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      2008-08-20
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 組換え体ヒトヒアルロニダーゼ-1および-2の基質特異性の研究2008

    • Author(s)
      山田修平
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      2008-08-20
    • Related Report
      2008 Final Research Report
  • [Presentation] イカ肝臓外皮由来のコンドロイチン硫酸の構造および生理活性の解析2008

    • Author(s)
      小林孝成
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      2008-08-19
    • Related Report
      2008 Final Research Report
  • [Presentation] 組換え体ヒトヒアルロニダーゼ-4 の基質特異性の研究2008

    • Author(s)
      山田修平
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      2008-08-19
    • Related Report
      2008 Final Research Report
  • [Presentation] 線虫Caenorhabditis elegansにおける新規のコンドロイチン加水分解酵素の同定2008

    • Author(s)
      山田修平
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      2008-08-19
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 組換え体ヒトピアルロニダーゼ-4の基質特異性の研究2008

    • Author(s)
      山田修平
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      2008-08-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] 組換え体ヒトピアルロニダーゼ-1および-2の基質特異性の研究2008

    • Author(s)
      山田修平
    • Organizer
      第28回日本糖質学会年会
    • Place of Presentation
      つくば
    • Year and Date
      2008-08-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] イカ肝臓外皮由来のコンドロイチン硫酸の構造と神経突起伸長促進活性2008

    • Author(s)
      小林孝成
    • Organizer
      日本生化学会北海道支部第45 回例会
    • Place of Presentation
      札幌
    • Year and Date
      2008-08-08
    • Related Report
      2008 Final Research Report
  • [Presentation] 線虫Caenorhabditis elegansにおける新規のコンドロイチン加水分解酵素の同定2008

    • Author(s)
      山田修平
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      2008-03-28
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 線虫Caenorhabditis elegansにおける新規のコンドロイチン加水分解酵素の同定2008

    • Author(s)
      金岩知之
    • Organizer
      第42回高分子学会北海道支部研究発表会
    • Place of Presentation
      札幌
    • Year and Date
      2008-01-29
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Chondroitinase-MediatedDegradation of Rare 3-O-SulfatedGlucuronic Acid in Functional OversulfatedChondroitin Sulfate Chains2007

    • Author(s)
      Ajaya K. Shetty
    • Organizer
      BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会合同大会)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] コンドロイチン硫酸(CS)/デルマタン硫酸(DS)中のグルクロン酸(GlcA)とイズロン酸(IdoA)の1H NMR解析による定量2007

    • Author(s)
      李福川
    • Organizer
      BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] Chondroitinase-Mediated Degradation of Rare 3-Ο-Sulfated Glucuronic Acid in Functional Oversulfated Chondroitin Sulfate Chains2007

    • Author(s)
      Ajaya K Shetty
    • Organizer
      BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] 単クローン抗体MO-225 が認識するコンドロイチン硫酸E由来のオリゴ糖の構造解析2007

    • Author(s)
      山田修平
    • Organizer
      第27回日本糖質学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2007-08-03
    • Related Report
      2008 Final Research Report
  • [Presentation] サメ軟骨コンドロイチン硫酸由来の八糖の構造解析と抗体のエピトープ解析への応用2007

    • Author(s)
      山田修平
    • Organizer
      第27回日本糖質学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2007-08-03
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 単クローン抗体MO-225が認識するコンドロイチン硫酸E由来のオリゴ糖の構造解析2007

    • Author(s)
      Sarama Sathyaseelan Deepa
    • Organizer
      第27回日本糖質学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2007-08-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] 抗コンドロイチン硫酸抗体のエピトープ構造2007

    • Author(s)
      菅原一幸
    • Organizer
      第27回日本糖質学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2007-08-01
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Chondroitinase-Mediated Degradation ofRare 3-O-Sulfated Glucuronic acid inFunctional Oversulfated Chondroitin SulfateChains2007

    • Author(s)
      Duriya Fongmoon
    • Organizer
      Glyco19(XIX InternationalSymposium on Glycoconjugates)
    • Place of Presentation
      Cairns
    • Year and Date
      2007-07-16
    • Related Report
      2008 Final Research Report
  • [Presentation] Chondroitinase-Mediated Degradation of Rare 3-Ο-Sulfated Glucuronic acid in Functional Oversulfated Chondroitin Sulfate Chains.2007

    • Author(s)
      D. Fongmoon
    • Organizer
      Glycol9(XIX International Symposium on Glycoconjugates)
    • Place of Presentation
      Cairns
    • Year and Date
      2007-07-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] 抗コンドロイチン硫酸D(CS-D)抗体MO-225が認識するCS-E由来のオリゴ糖の構造解析2007

    • Author(s)
      山田修平
    • Organizer
      第44回日本生化学会北海道支部例会
    • Place of Presentation
      札幌
    • Year and Date
      2007-07-06
    • Related Report
      2008 Final Research Report
  • [Presentation] 抗コンドロイチン硫酸D(CS-D)抗体MO-225が認識するCS-E由来のオリゴ糖の構造解析2007

    • Author(s)
      Sarama Sathyaseelan Deepa
    • Organizer
      第44回 日本生化学会北海道支部例会
    • Place of Presentation
      札幌
    • Year and Date
      2007-07-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] コンドロイチン硫酸(CS)/デルマタン硫酸(DS)中のグルクロン酸(GlcA)とイズロン酸(IdoA)の1HNMR解析による定量2004

    • Author(s)
      山田修平
    • Organizer
      BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会合同大会)
    • Place of Presentation
      横浜
    • Year and Date
      2004-12-12
    • Related Report
      2008 Final Research Report
  • [Book] Springer, Tokyo2008

    • Author(s)
      Kazuyuki Sugahara and Shuhei Yamada
    • Publisher
      Experimental Glycoscience Glycochemistry,
    • Related Report
      2008 Final Research Report
  • [Book] Experimental Glycoscience Glycochemistry2008

    • Author(s)
      Kazuyuki Sugahara
    • Publisher
      Springer, Tokyo
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] ムコ多糖分解促進剤2007

    • Inventor(s)
      菅原一幸, 山田修平, 水本秀二
    • Industrial Property Rights Holder
      北海道大学
    • Filing Date
      2007-11-09
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] ムコ多糖分解促進剤2007

    • Inventor(s)
      菅原一幸, 山田修平, 水本秀二
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2007-291424
    • Filing Date
      2007-11-09
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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