Spacio-temporal analysis of molecular sorting events in mammalian trans-Golgi network and endosomes

• Project/Area Number: 19590193
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Fukushima Medical University
• Principal Investigator: WAGURI Satoshi Fukushima Medical University, 医学部, 教授 (30244908)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: TGN / エンドソーム / 小胞輸送 / クラスリン / GGA / マンノース6リン酸受容体 / mannose 6-phosphate receptor / RNAi / transGolgi Netowork / endosome / トランスゴルジネットワーク / クラスリンアダプター / ライブセルイメージング

Research Abstract

本課題では、細胞内のトランスゴルジネットワーク(TGN)からエンドソームへの小胞輸送を制御する単量体クラスリンアダプタータンパク質であるGGAsの機能について、ライブセルイメージング法およびRNAi法等を用いて詳細に解析した。その結果、GGAがカーゴタンパク質であるカチオン非依存性マンノース6リン酸受容体のTGNにおける選別過程のみならず、同分子のターンオーバーに関わることを明らかにした。

Research Products

• [Journal Article] Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice (2009)
  • Author(s): Waguri S and Komatsu M
  • Journal Title: Methods Enzymol 453 Pages: 181-196
  • Peer Reviewed
• [Journal Article] Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation (2009)
  • Author(s): Gotoh K, Lu Z, Morita M, Shibata M, Koike M, Waguri S, Dono K, Doki Y, Kominami E, Sugioka A, Monden M, Uchiyama Y
  • Journal Title: Autophagy 5 Pages: 351-360
  • Peer Reviewed
• [Journal Article] Three homologous ArfGAPs participate in COPI-mediated transport. (2009)
  • Author(s): Akina Saitoh, et al.
  • Journal Title: Journal of Biological Chemistry (in press)
  • Peer Reviewed
• [Journal Article] Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice. (2009)
  • Author(s): Waguri, Komatsu
  • Journal Title: Methods in Enzymology 453 Pages: 181-196
  • Peer Reviewed
• [Journal Article] The atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice (2008)
  • Author(s): Sou YS, Waguri S(equal contribution), Iwata JI, Ueno T, Fujimura T, Hara T, Sawada N, Yamada A, Mizushima N, Uchiyama Y, Kominami E, Tanaka K, Komatsu M
  • Journal Title: Mol Biol Cell 19 Pages: 4762-4775
  • Peer Reviewed
• [Journal Article] Human RME-8 is involved in membrane trafficking through early endosome (2008)
  • Author(s): Fujibayashi A, Taguchi T, Misaki R, Ohtani M, Dohmae N, Takio K, Yamada M, Gu J, Yamakami M, Fukuda M, Waguri S, S, Uchiyama Y, Yoshimori T, Sekiguchi K.
  • Journal Title: Cell Struct Funct 33 Pages: 35-50
  • Peer Reviewed
• [Journal Article] Inhibition of Autophagy Prevents Hippocampal Pyramidal Neuron Death after Hypoxic-Ischemic Injury (2008)
  • Author(s): Koike M, Shibata M, Tadakoshi M, Gotoh K, Komatsu M, Waguri S, Kawahara N, Kuida K, Nagata S, Kominami E, Tanaka K, Uchiyama Y.
  • Journal Title: Am J Pathol 172 Pages: 454-469
  • Peer Reviewed
• [Journal Article] The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice (2008)
  • Author(s): Waguri, et al.
  • Journal Title: Molecular Biology of the Cell 19 Pages: 4762-4775
  • Peer Reviewed
• [Journal Article] Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells. (2008)
  • Author(s): Tatsuhiro Hida
  • Journal Title: Archives of Histology and Cytology (印刷中)
  • Peer Reviewed
• [Journal Article] Human RME-8 is involved in membrane trafficking through early endosome. (2008)
  • Author(s): Akemi Fujibayashi
  • Journal Title: Cell Structure and Function (印刷中)(公開日2008/02/06)
  • Peer Reviewed
• [Journal Article] Homeostatic Levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice (2008)
  • Author(s): Masaaki Komatsu
  • Journal Title: Cell 131 Pages: 1149-1163
  • Peer Reviewed
• [Journal Article] Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells (2007)
  • Author(s): Hida T, Ikeda H, Kametaka S, Akazawa C, Kohsaka S, Ebisu S, Uchiyama Y, Waguri S
  • Journal Title: Arch Histol Cytol 70 Pages: 303-312
  • Peer Reviewed
• [Journal Article] Rab-small GTPases are involved in fluvastatin and pravastatin-induced vacuolation in rat skeletal myofibers (2007)
  • Author(s): Sakamoto K, Honda T, Yokoya S, Waguri S, Kimura J
  • Journal Title: FASEB J 21 Pages: 4087-4094
  • Peer Reviewed
• [Journal Article] Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice (2007)
  • Author(s): Komatsu M, Waguri S, Koike M, Sou Y, Ueno T, Hara T, Mizushima N, Iwata J, Ezaki J, Murata S, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T, Uwayama J, Warabi E, Yoshida H, Ishii T, Kobayashi A, Yamamoto M, Yue Z, Uchiyama Y, Kominami E, Tanaka K
  • Journal Title: Cell 131 Pages: 1149-1163
  • Peer Reviewed
• [Presentation] 高等動物におけるオートファジーの生理機能と隔離膜成熟機構 (2009)
  • Author(s): 和栗聡
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-30
• [Presentation] マウス組織におけるβKlothoの発現分布解析 (2009)
  • Author(s): 山本雅哉, 亀高諭, 和栗聡
  • Organizer: 第113回日本解剖学会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-29
• [Presentation] ショウジョウバエ細胞を用いたクラスリンアダプターの機能解析 (2009)
  • Author(s): 亀高諭, 和栗聡
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-28
• [Presentation] GFP-MPR融合タンパク質を安定に発現するHeLa 細胞を用いたGGA3の機能解析 (2009)
  • Author(s): 澤田直樹, 亀高諭, 池田寛子, 笹本まどか, 和栗聡
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-28
• [Presentation] GFP-MPR融合タンパク質を安定に発現するHeLa細胞を用いたGGA3の機能解析 (2009)
  • Author(s): 深田直樹
  • Organizer: 第114回 日本解剖学会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-28
• [Presentation] Different phenoty pescaused by specific depletion of GGA1, 2, or 3in HeLa cells (2008)
  • Author(s): 池田寛子, 亀高諭, 和栗聡
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-30
• [Presentation] HeLa細胞で見られたGGA1, 2, 3の特異的発現低下による表現形の違い (2008)
  • Author(s): 池田寛子, 亀高諭, 和栗聡
  • Organizer: 第114回日本解剖学会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-27
• [Presentation] HeLa細胞で見られたGGA1,2,3の特異的発現低下よる表現型の違い (2008)
  • Author(s): 池田 寛子
  • Organizer: 第113回 日本解剖学会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-27
• [Presentation] The luminal domain paticipates in the endosomal trafficking of the cation-independent mannose 6-phosphate receptor (2007)
  • Author(s): 和栗聡, 富山雄人, 池田寛子, 飛田達宏, 酒井規夫、谷池雅子, 恵比寿繁之, 内山安男
  • Organizer: 第59回日本細胞生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-30
• [Presentation] 光学顕微鏡で捉えるTGN-エンドソーム間のリサイクリング輸送 (2007)
  • Author(s): 和栗聡
  • Organizer: 第63回日本顕微鏡学会
  • Place of Presentation: 新潟
  • Year and Date: 2007-05-02
• [Remarks]
  • URL: http://www.fmu.ac.jp/home/anatomy2/anatomy2_index.html
• [Remarks]
  • URL: http://www.fmu.ac.jp/kenkyu/Profiles/5/0000466/profile.html