• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of glutathione-mediated regulation of nitrative signal in cells

Research Project

Project/Area Number 19590253
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionKumamoto University

Principal Investigator

FUJII Shigemoto  Kumamoto University, 大学院・医学薬学研究部, 助教 (00325333)

Co-Investigator(Kenkyū-buntansha) AKAIKE Takaaki  熊本大学, 大学院・医学薬学研究部, 教授 (20231798)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords一酸化窒素 / 活性酸素 / 酸化ストレス / グルタチオン / ニトロ化 / サイクリックGMP / 8-ニトロcGMP / S-グアニル化
Research Abstract

活性酸素や一酸化窒素によってもたらされる酸化ストレスは、様々な細胞機能変化をもたらす。その機序の一つである細胞内ニトロ化シグナルについて、グルタチオンによる制御機構に焦点をあて解析を行った。その結果、グルタチオンは、ニトロ化シグナルを担う8-ニトロcGMPの生成とこの分子による蛋白質の翻訳後修飾を調節し、ヘムオキシゲナーゼ-1などの抗酸化蛋白質の発現を介した酸化ストレスに対する適応反応の制御に関与していることが示唆された。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (46 results)

All 2009 2008 2007 Other

All Journal Article (25 results) (of which Peer Reviewed: 19 results) Presentation (18 results) Remarks (3 results)

  • [Journal Article] Cytoprotective function of heme oxygenase1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis2009

    • Author(s)
      Zaki MH, Fujii S, Okamoto T, Islam S, Khan S, Ahmed KA, Sawa T, and Akaike T.
    • Journal Title

      JImmunol 182

      Pages: 3746-56

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] NOによる細胞内感染防御の新しい展開2009

    • Author(s)
      赤池孝章、岡本竜哉、Zaki MH、藤井重元、澤智裕
    • Journal Title

      日本ハンセン病学会雑誌 78

      Pages: 41-47

    • NAID

      10024786455

    • Related Report
      2008 Final Research Report
  • [Journal Article] 活性酸素・NOによる感染防御シグナルの新展開2009

    • Author(s)
      岡本竜哉, 澤智裕, 藤井重元, 赤池孝章
    • Journal Title

      細胞 41

      Pages: 51-55

    • Related Report
      2008 Final Research Report
  • [Journal Article] Cytoprotective function of heme oxygenase 1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis.2009

    • Author(s)
      Zaki, et al
    • Journal Title

      J Immunol 182

      Pages: 3746-3756

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 8-Nitroguanosines as chemical probes of the protein S-guanylation2008

    • Author(s)
      Saito Y, Taguchi H, Fujii S, Sawa T, Kida E, Kabuto C, Akaike T, Arimoto T.
    • Journal Title

      Chem Commun

      Pages: 5984-5986

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] S-nitrosylated human serum albumin-mediated cytoprotective activity is enhanced by fatty acid binding2008

    • Author(s)
      Ishima Y, Akaike T, Kragh-Hansen U, Hiroyama S, Sawa T, Suenaga A, Maruyama T, and Otagiri M.
    • Journal Title

      J Biol Chem 283

      Pages: 34966-34975

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitric oxide regulation of MMP-9 activation and its relationship to modifications of the cysteine switch2008

    • Author(s)
      McCarthy SM, Bove PF, Matthews DE, Akaike T, and van der Vliet A.
    • Journal Title

      Biochemistry 47

      Pages: 5832-5840

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of NO production and 8-nitroguanosine formation byphenol-containing endocrine-disrupting chemicals in LPS-stimulated macrophages : Involvement of estrogen receptor-dependent or -independent pathways2008

    • Author(s)
      Yoshitake J, Kato K, Yoshioka D, Sueishi Y, Sawa T, Akaike T, and Yoshimura T.
    • Journal Title

      Nitric Oxide 18

      Pages: 223-228

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Angiopoietin-related growth factor enhances blood flow via activation of the ERK1/2-eNOS-NO pathway in a mouse hind-limb ischemia model2008

    • Author(s)
      Urano T, Ito Y, Akao M, Sawa T, Miyata K, Tabata M, Morisada T, Hato T, Yano M, Kadomatsu T, Yasunaga K, Shibata R, Murohara T, Akaike T, Tanihara H, Suda T, and Oike Y.
    • Journal Title

      Arterioscler Thromb Vasc Biol. 28

      Pages: 827-834

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitric oxide produced in Peyer's patches exhibits antiapoptotic activity contributing to an antimicrobial effect in murine salmonellosis2008

    • Author(s)
      Alam MS, Zaki MH, Sawa T, Islam S, Ahmed KA, Fujii S, Okamoto T, and Akaike T.
    • Journal Title

      Microbiol Immunol 52

      Pages: 197-208

    • NAID

      10025691687

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutagenicity of 8-nitroguanosine, a product of nitrative nucleoside modification by reactive nitrogen oxides in mammalian cells2008

    • Author(s)
      Kaneko K, Akuta T, Sawa T, Kim HW, Fujii S, Okamoto T, Nakayama H, Ohigashi H, Murakami A, and Akaike T.
    • Journal Title

      Cancer Lett 262

      Pages: 239-47

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 肺の感染炎症病態におけるニトロ化ストレスとそのバイオマーカー2008

    • Author(s)
      岡本竜哉, 赤池孝章
    • Journal Title

      医学のあゆみ 224

      Pages: 851-6

    • Related Report
      2008 Final Research Report
  • [Journal Article] 感染病態におけるNO・活性酸素のシグナル伝達機能 : 酸化ストレスとその適応応答の分子メカニズム2008

    • Author(s)
      岡本竜哉, 藤井重元, 澤智裕, 赤池孝章
    • Journal Title

      Allergy From the Nose to the Lung 6

      Pages: 12-7

    • Related Report
      2008 Final Research Report
  • [Journal Article] 活性酸素を消去する物質8-ニトロcGMP2008

    • Author(s)
      澤智裕、赤池孝章
    • Journal Title

      検査と技術 36

      Pages: 678-679

    • Related Report
      2008 Final Research Report
  • [Journal Article] Nitric oxide produced in Peyer's patches exhibits antiapoptotic activity contributing to an antimicrobial effect in murine salmonellosis.2008

    • Author(s)
      Alam, et al
    • Journal Title

      Microbiol Immunol 52

      Pages: 197-208

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutagenicity of 8-nitroguanosine, a product of nitrative nucleoside modification by reactive nitrogen oxides, in mammalian cells.2008

    • Author(s)
      Kaneko, et al
    • Journal Title

      Cancer Lett 262

      Pages: 239-247

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 8-Nitroguanosines as chemical probes of the protein S-guanylation.2008

    • Author(s)
      Saito, et al
    • Journal Title

      Chem Commun

      Pages: 5984-5986

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutagenicity of 8-nitroguanosine, a product of nitrative nucleotide modification by reactive nitrogen oxides, in mammalian cells.2008

    • Author(s)
      Kaneko, et. al.
    • Journal Title

      Cancer Lett 262

      Pages: 239-247

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitric oxide produced in Peyer's patches exhibits antiapoptotic activity contributing to an antimicrobial effect in murine salmonellosis.2008

    • Author(s)
      Alam, et. al.
    • Journal Title

      Microbiol Immunol (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Endogenous ligands on the biological role of human serum albumin in S-nitrosylation2007

    • Author(s)
      Ishima Y, Akaike T, Kragh-Hansen U, Hiroyama S, Sawa T, Maruyama T, Kai T, and Otagiri M.
    • Journal Title

      Biochem Biophys Res Commun 364

      Pages: 790-795

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein S-guanylation by the biological signal 8-nitroguanosine 3', 5'-cyclic monophosphate2007

    • Author(s)
      Sawa T, Zaki MH, Okamoto T, Akuta T, Tokutomi Y, Kim-Mitsuyama S, Ihara H, Kobayashi A, Yamamoto M, Fujii S, Arimoto H, and Akaike T.
    • Journal Title

      Nature Chem Biol 3

      Pages: 727-35

    • NAID

      120002467822

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Albuminuria, expression of nitotinamide adenine dinucleotide phosphate oxidase and monocyte chemoattractant protein-1 in therenal tubules of hypertensive Dahl salt-sensitive rats2007

    • Author(s)
      Fujii S, Zhang L, and Kosaka H.
    • Journal Title

      Hypertens Res. 30

      Pages: 991-8

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitrative stress in respiratory inflammation caused by influenza virusinfection2007

    • Author(s)
      Zaki MH, Okamoto T, Sawa T, Fujii S, and Akaike T.
    • Journal Title

      Clin Exp Allergy Rev 7

      Pages: 19-26

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein S-guanylation by the biological signal 8-nitroguanosin 3',5'-cyclic monophosphate.2007

    • Author(s)
      Sawa T., et. al.
    • Journal Title

      Nature Chemical Biology 3

      Pages: 727-735

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrative stress in respiratory inflammation caused by influenza virus infection.2007

    • Author(s)
      Zaki M. H., et. al.
    • Journal Title

      Clinical and Experimental Allergy Reviews 7

      Pages: 19-26

    • Related Report
      2007 Annual Research Report
  • [Presentation] サルモネラ感染におけるニトロ化環状ヌクレオチドを介する生体防御機構2009

    • Author(s)
      藤井重元
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-03-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] サルモネラ感染におけるニトロ化環状ヌクレオチドを介する生体防御機構2009

    • Author(s)
      藤井重元、岡本竜哉、澤智裕、赤池孝章
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋市
    • Related Report
      2008 Final Research Report
  • [Presentation] 新規環状ヌクレオチド8-ニトログアノシン-3', 5'-環状1リン酸による転写制御因子Keap1のs-グアニル化とその酸化ストレス応答における役割2008

    • Author(s)
      藤井重元
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Keap1のS-グアニル化を介する環状ヌクレオチド8-ニトログアノシン-3', 5'-環状1リン酸のシグナル伝達機序2008

    • Author(s)
      藤井重元
    • Organizer
      第61回日本酸化ストレス学会学術集会
    • Place of Presentation
      国立京都国際会館アネックスホール
    • Year and Date
      2008-06-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 新規環状ヌクレオチド8-ニトログアノシン-3', 5'-環状1リン酸による転写制御因子Keap1のS-グアニル化とそのシグナル伝達機構2008

    • Author(s)
      藤井重元
    • Organizer
      第8回日本NO学会学術集会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2008-05-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 新規環状ヌクレオチド8-ニトログアノシン3', 5'-環状1リン酸による転写制御因子Keap1のS-グアニル化とその酸化ストレス応答における役割2008

    • Author(s)
      藤井重元、澤智裕、岡本竜哉、居原秀、本橋ほづみ、山本雅之、赤池孝章
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸市
    • Related Report
      2008 Final Research Report
  • [Presentation] サルモネラ感染病態における8-ニトログアノシン3', 5'-環状1リン酸を介したNO誘導性生体防御機構2008

    • Author(s)
      藤井重元、Md. H. Zaki、岡本竜哉、澤智裕、赤池孝章
    • Organizer
      第61回日本細菌学会九州支部総会
    • Place of Presentation
      熊本市
    • Related Report
      2008 Final Research Report
  • [Presentation] Signal transduction by 8-nitro-cGMP via its unique redox property and post-translational modification2008

    • Author(s)
      Akaike T, and Sawa T.
    • Organizer
      A Joint Conference of 13th In Vivo EPR Spectroscopy and Imaging and 10th International EPR Spin Trapping/Spin Labeling
    • Place of Presentation
      福岡市
    • Related Report
      2008 Final Research Report
  • [Presentation] Protein S-guanylation induced by 8-nitro-cGMP2008

    • Author(s)
      Akaike T.
    • Organizer
      5^<th> Internal conference on biology, chemistry and therapeutic applications of NO
    • Place of Presentation
      Bregenz, Austria
    • Related Report
      2008 Final Research Report
  • [Presentation] Physiological role of Keap1 S-guanylation by 8-nitroguanosine 3', 5'-cyclic moophosphate formed in C6 glioma cells2008

    • Author(s)
      Fujii S, Sawa T, Okamoto T, Ihara H, Motohashi H, Yamamoto M, and Akaike T.
    • Organizer
      5^<th> Internal conference on biology, chemistry and therapeutic applications of NO
    • Place of Presentation
      Bregenz, Austria
    • Related Report
      2008 Final Research Report
  • [Presentation] Keap1のS-グアニル化を介する環状ヌクレオチド8-ニトログアノシン3', 5'-環状1リン酸のシグナル伝達機序2008

    • Author(s)
      藤井重元、澤智裕、岡本竜哉、居原秀、小林聡、山本雅之、赤池孝章
    • Organizer
      第61回日本酸化ストレス学会学術集会
    • Place of Presentation
      京都市
    • Related Report
      2008 Final Research Report
  • [Presentation] 新規環状ヌクレオチド8-ニトログアノシン3', 5'-環状1リン酸による転写制御因子Keap1のS-グアニル化とそのシグナル伝達機構2008

    • Author(s)
      藤井重元、澤智裕、岡本竜哉、居原秀、小林聡、山本雅之、赤池孝章
    • Organizer
      第8回日本NO学会学術集会
    • Place of Presentation
      仙台市
    • Related Report
      2008 Final Research Report
  • [Presentation] Physiological role of Keap 1 S-guanylation by 8-nitroguanosine 3', 5'-cyclic monophosphate formed in C6 glioma cells2008

    • Author(s)
      Shigemoto Fujii
    • Organizer
      5th International Conference : □ Biology, Chemistry and Therapeutic □ Applieations of Nitric Oxide
    • Place of Presentation
      Bregenz, Austria
    • Related Report
      2008 Annual Research Report
  • [Presentation] 新規環状ヌクレオチド8-ニトログアノシン-3',5'-環状1リン酸による転写制御因子Keap1のS-グアニル化とその生理作用2007

    • Author(s)
      藤井 重元
    • Organizer
      第29回日本フリーラジカル学会学術集会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-06-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] 転写制御因子Keap1のS-グアニル化を介した新規環状ヌクレオチド8-ニトログアノシン-3',5'-環状1リン酸による細胞保護作用2007

    • Author(s)
      藤井 重元
    • Organizer
      第7回日本NO学会学術集会
    • Place of Presentation
      滋賀県大津市ピアザ淡海
    • Year and Date
      2007-05-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] A new second messenger, 8-nitroguanosine 3', 5'-cyclic monophosphate involved in NO-induced signal transduction via a unique post-translational modification, protein S-guanylation2007

    • Author(s)
      Akaike T. Sawa T, Fujii S, Okamoto T, Ihara H, and Arimoto H.
    • Organizer
      4th Joint Meeting of the Society for Free Radical Research, Australasia and Japan
    • Place of Presentation
      京都市
    • Related Report
      2008 Final Research Report
  • [Presentation] 新規環状ヌクレオチド8-ニトログアノシン3', 5'-環状1リン酸による転写制御因子Keap1のS-グアニル化とその生理作用2007

    • Author(s)
      藤井重元、澤智裕、Mohammad Hasan Zaki、赤池孝章
    • Organizer
      第29回日本フリーラジカル学会学術集
    • Place of Presentation
      名古屋市
    • Related Report
      2008 Final Research Report
  • [Presentation] 転写制御因子Keap1のS-グアニル化を介した新規環状ヌクレオチド8-ニトログアノシン3', 5'-環状1リン酸による細胞保護作用2007

    • Author(s)
      藤井重元、澤智裕、岡本竜哉、HASAN ZAKI MUHAMMAD、赤池孝章
    • Organizer
      第7回日本NO学会学術集会
    • Place of Presentation
      大津市
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://kumadai-bisei.com

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://kumadai-bisei.com/

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://kumadai-bisei.com.

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi