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Angiogenic potential of multipotent neural stem cells

Research Project

Project/Area Number 19590262
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionMeiji Pharmaceutical University

Principal Investigator

OISHI Kazuhiko  Meiji Pharmaceutical University, 薬学部, 教授 (80203701)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords神経幹細胞 / 内皮細胞 / 血管腔構造 / 三次元培養 / 分化 / 脳血管 / 平滑筋細胞 / β-catenin / TCF / コラーゲン / GSK3B
Research Abstract

We found that CD44^+ CD90^+ cells isolated from primary neurospheres can form vascular tube structures in vitro. NSCs isolated from the mouse embryonic cortex formed neurospheres when cultured in serum-free medium containing 20ng/ml basic fibroblast growth factor (bFGF). CD44^+ CD90^+ cells were enriched from the neurospheres using an EPICS ALTRA flow cytometer, and antibodies against CD44 and CD90. The purified CD44^+ CD90^+ cells generated neurospheres, and differentiated into neurons and astrocytes. When the cells were inoculated into collagen gels and cultured with 20% fetal bovine serum plus bFGF for 7 d, vascular tube-like structures were formed. These results indicate that CD44^+ CD90^+ cells have the ability to generate neurospheres and to form vascular tubes.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report

Research Products

(12 results)

All 2009 2008 2007 2006

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results)

  • [Journal Article] Isometric contraction of microvascular pericytes from mouse brain parenchyma.2007

    • Author(s)
      K. Oishi, T. Kamiyashiki, Y. Ito
    • Journal Title

      Microvasc Res. 73

      Pages: 20-28

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro development of gut-like tissue demonstrating rhythmic contractions from embryonic mouse intestinal cells.2007

    • Author(s)
      Y. Ito-Dufros, Y. Funakoshi, A. Uehara, K. Oishi
    • Journal Title

      Neurogastroenterol Motil. 19

      Pages: 288-300

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isometrie contraetion of microvascular pericytes from mouse brain parenchyma2007

    • Author(s)
      Oishi K, Kamiyashiki T, and Ito Y.
    • Journal Title

      Microvase res 73

      Pages: 20-28

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro development of gut-like tissue demonstrating rhythmic contractions from embryonic mouse intestinal cells2007

    • Author(s)
      Ito-Duyfros Y, Funakoshi Y, Uehara A, and Oishi K.
    • Journal Title

      Neurogastroenterol Motil 19

      Pages: 288-800

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Angiogenic potential of CD44+ CD90+ multipotent CNS stem cells in vitro.2006

    • Author(s)
      K. Oishi, Y. Ito-Dufros
    • Journal Title

      Biochem Biophys Res Commun. 349

      Pages: 1065-1072

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] 幹細胞から作製する再構成組織標本とその薬理学的解析への応用2009

    • Author(s)
      大石一彦
    • Organizer
      第22回日本動物実験代替法学会総会・学術大会
    • Place of Presentation
      大阪大学(大阪市)
    • Year and Date
      2009-11-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] iPS細胞の分化と薬理学研究への応用2009

    • Author(s)
      大石一彦
    • Organizer
      第36回日本トキシコロジー学会学術年会
    • Place of Presentation
      アイーナ(盛岡市)
    • Year and Date
      2009-07-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] iPS細胞の分化と薬理学研究への応用2009

    • Author(s)
      大石一彦
    • Organizer
      第36回日本トキシコロジー学会学術年会
    • Place of Presentation
      盛岡
    • Related Report
      2009 Final Research Report
  • [Presentation] 幹細胞から作製する再構成組織標本とその薬理学的解析への応用2009

    • Author(s)
      小川泰弘、大石一彦
    • Organizer
      第22回日本動物実験代替法学会総会・学術大会
    • Place of Presentation
      大阪(シンポジウム講演)
    • Related Report
      2009 Final Research Report
  • [Presentation] Microvascular Pericytes from Mouse Brain Parenchyma Contract Isometrically.2008

    • Author(s)
      Oishi K
    • Organizer
      The 15^<th> International Vascular Biology Meeting
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2008-06-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Microvascular Pericytes from Mouse Brain Parenchyma Contract Isometrically.2008

    • Author(s)
      Oishi K.
    • Organizer
      The 15th International Vascular Biology Meeting
    • Place of Presentation
      Sydney, Australia (workshop)
    • Related Report
      2009 Final Research Report
  • [Presentation] Neuroangiogenic potential of neural stem cells.2007

    • Author(s)
      Oishi K
    • Organizer
      The JSPS 2nd Medicinal Chemistry Seminor of Asia/Africa Science Platform Program
    • Place of Presentation
      Phuket, Thailand (an invited lecture)
    • Related Report
      2009 Final Research Report

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Published: 2007-03-31   Modified: 2016-04-21  

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