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Identification of sub-femto mole protein from gel electrophoresis

Research Project

Project/Area Number 19590275
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKyoto University

Principal Investigator

OKAWA Katsuya  Kyoto University, 医学研究科, その他(グループリーダー) (20444458)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords生体分子医学 / 蛋白質 / プロテオーム / 分析科学 / 電気泳動 / サブフェムトモル
Research Abstract

従来法の要素技術の検証を進め、改善点が見出されて来ている.これまでに開発した、従来の10倍程度以上高感度なゲル内タンパク質染色法を活用し、本方法でなければ可視化出来ないサブフェムトモルのモデルとなるタンパク質を調製し、少なくともサブフェムトモルレベルでは従来法の延長線上の解析手法にて対応可能であることが見出された.
また、得られた検証結果を随時、実践のタンパク質解析に応用している.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (44 results)

All 2009 2008 2007 Other

All Journal Article (26 results) (of which Peer Reviewed: 26 results) Presentation (16 results) Remarks (2 results)

  • [Journal Article] mDia2 shuttles between the nucleus and the cytoplasm through the Importin-{alpha}/{beta}- and CRM1-mediated nuclear transport mechanism2009

    • Author(s)
      Miki T, Okawa K, Sekimoto T, Yoneda Y, Watanabe S, Ishizaki T, Narumiya S
    • Journal Title

      J Biol Chem 284(9)

      Pages: 5753-62

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of postsplicing lariat-intron complexes2009

    • Author(s)
      Yoshimoto R, Kataoka N, Okawa K, Ohno M
    • Journal Title

      Nucleic Acids Res 37(3)

      Pages: 891-902

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-moesin antibodies in the serum of patients with aplastic anemia stimulate peripheral blood mononuclear cells to secrete TNF-alpha and IFN-gamma2009

    • Author(s)
      Takamatsu H, Espinoza JL, Lu X, Qi Z, Okawa K, Nakao S
    • Journal Title

      J Immunol 182(1)

      Pages: 703-10

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] RECK forms cowbell-shaped dimers and inhibits matrix metalloproteinase-catalyzed cleavage of fibronectin2009

    • Author(s)
      Omura A, Matsuzaki T, Mio K, Ogura T, Yamamoto M, Fujita A, Okawa K, Kitayama H, Takahashi C, Sato C, Noda M
    • Journal Title

      J Biol Chem 284(6)

      Pages: 3461-9

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of post-splicing lariat-intron complex2009

    • Author(s)
      Yoshimoto R, ほか
    • Journal Title

      Nucleic Acids Res. 37

      Pages: 891-902

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore2008

    • Author(s)
      Hori T, Amano M, Suzuki A, Backer CB, Welburn JP, Dong Y, McEwen BF, Shang WH, Suzuki E, Okawa K, Cheeseman IM, Fukagawa T
    • Journal Title

      Cell 135(6)

      Pages: 1039-52

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] IQGAP3 regulates cell proliferation through the Ras/ERK signalling cascade2008

    • Author(s)
      Nojima H, Adachi M, Matsui T, Okawa K, Tsukita S, Tsukita S
    • Journal Title

      Nat Cell Biol 10(8)

      Pages: 971-8

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP2008

    • Author(s)
      Hosokawa N, Wada I, Nagasawa K, Moriyama T, Okawa K, Nagata K
    • Journal Title

      J Biol Chem 283(30)

      Pages: 20914-24

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optimized proteomic analysis on gels of cell-cell adhering junctional membrane proteins2008

    • Author(s)
      Yamazaki Y, Okawa K, Yano T, Tsukita S, Tsukita S
    • Journal Title

      Biochemistry 47(19)

      Pages: 5378-86

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Loss of Borealin/DasraB leads to defective cell proliferation, p53 accumulation and early embryonic lethality2008

    • Author(s)
      Yamanaka Y, Heike T, Kumada T, ShibataM, Takaoka Y, Kitano A, Shiraishi K, Kato T, Nagato M, Okawa K, Furushima K, Nakao K, Nakamura Y, Taketo MM, Aizawa S, Nakahata T
    • Journal Title

      Mech Dev 125(5-6)

      Pages: 441-50

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization of the Rho GTPase-regulated actin assembly by diaphanous-related formins, mDia1 and Daam1, in platelets2008

    • Author(s)
      Higashi T, Ikeda T, Shirakawa R, Kondo H, Kawato M, Horiguchi M, Okuda T, Okawa K, Fukai S, Nureki O, Kita T, Horiuchi H
    • Journal Title

      J Biol Chem 283(13)

      Pages: 8746-55

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] MKKS is a centrosome-shuttling protein degraded by disease-causing mutations via CHIP-mediated ubiquitination2008

    • Author(s)
      Hirayama S, Yamazaki Y, Kitamura A, Oda Y, Morito D, Okawa K, Kimura H, Cyr DM, Kubota H, Nagata K.
    • Journal Title

      Mol Biol Cell 19(3)

      Pages: 899-911

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of platelet dense granule secretion by the Ral GTPase-exocyst pathway2008

    • Author(s)
      Kawato M, Shirakawa R, Kondo H, Higashi T, Ikeda T, Okawa K, Fukai S, Nureki O, Kita T, Horiuchi H
    • Journal Title

      J Biol Chem 283(1)

      Pages: 166-74

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] IQGAP3 regulates cell proliferation through the Ras/ERK signalling cascade.2008

    • Author(s)
      Nojima H, ほか
    • Journal Title

      Nat Cell Biol. 10

      Pages: 971-8

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore.2008

    • Author(s)
      Hori T, ほか
    • Journal Title

      Cell. 135

      Pages: 1039-52

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MKKS Is a Centrosome-shuttling Protein Degraded by Disease-causing Mutations via CHIP-mediated Ubiquitination.2008

    • Author(s)
      Hirayama S, ほか
    • Journal Title

      Mol Biol Cell 19

      Pages: 899-911

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans2007

    • Author(s)
      Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H.
    • Journal Title

      EMBO J 26(24)

      Pages: 5007-19

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of alpha-secretase cleavage of amyloid precursor protein by a metalloendopeptidase nardilysin2007

    • Author(s)
      Hiraoka Y, Ohno M, Yoshida K, Okawa K, Tomimoto H, Kita T, Nishi E
    • Journal Title

      J Neurochem 102(5)

      Pages: 1595-605

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heat-shock protein 105 interacts with and suppresses aggregation of mutant Cu/Zn superoxide dismutase : clues to a possible strategy for treating ALS2007

    • Author(s)
      Yamashita H, Kawamata J, Okawa K, Kanki R, Nakamizo T, Hatayama T, Yamanaka K, Takahashi R, Shimohama S
    • Journal Title

      J Neurochem 102(5)

      Pages: 1497-505

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Factors associated with a purine-rich exonic splicing enhancer sequence in Xenopus oocyte nucleus2007

    • Author(s)
      Masuyama K, Taniguchi I, Okawa K, OhnoM
    • Journal Title

      Biochem Biophys Res Commun 359(3)

      Pages: 580-5

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Control of immune responses by antigen-specific regulatory T cells expressing the folate receptor2007

    • Author(s)
      Yamaguchi T, Hirota K, Nagahama K, Okawa K, Takahashi T, Nomura T, Sakaguchi S
    • Journal Title

      Immunity 27(1)

      Pages: 145-59

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gab family proteins are essential for postnatal maintenance of cardiac function via neuregulin-1/ErbB signaling2007

    • Author(s)
      Nakaoka Y, Nishida K, Narimatsu M, Kamiya A, Minami T, Sawa H, Okawa K, Fujio Y, Koyama T, Maeda M, Sone M, Yamasaki S, Arai Y, Koh GY, Kodama T, Hirota H, Otsu K, Hirano T, Mochizuki N
    • Journal Title

      J Clin Invest 117(7)

      Pages: 1771-81

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cyclin-dependent kinaselike 5 is a novel target of immunotherapy in adult T-cell leukemia2007

    • Author(s)
      Kawahara M, Hori T, Matsubara Y, Okawa K, Uchiyama T
    • Journal Title

      J Immunother 30(5)

      Pages: 499-505

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The reversion-inducing cysteine-rich protein with Kazal motifs(RECK)interacts with membrane type 1 matrix metalloproteinase and CD13/aminopeptidase N and modulates their endocytic pathways2007

    • Author(s)
      Miki T, Takegami Y, Okawa K, Muraguchi T, Noda M, Takahashi C
    • Journal Title

      J Biol Chem 282(16)

      Pages: 12341-52

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gab family proteins are essential for postnatal maintenance of cardiac function via neuregulin-1/ErbB signaling.2007

    • Author(s)
      Nakaoka Y, ほか
    • Journal Title

      J Clin Invest 117

      Pages: 1771-81

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of immune responses by antigen-specific regulatory Tcells expressing the folate receptor.2007

    • Author(s)
      Yamaguchi T, ほか
    • Journal Title

      Immunity 27

      Pages: 145-59

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] IQGAP3 regulates cell proliferation through the Ras/ERK signaling cascade2008

    • Author(s)
      H Nojima, ほか
    • Organizer
      BMB
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] 新規レクチンによる小胞体タンパク質品質管理機構2008

    • Author(s)
      細川暢子, ほか
    • Organizer
      BMB
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] Daam1、mDia1によるアクチン重合反応制御機構2008

    • Author(s)
      東智仁, ほか
    • Organizer
      BMB
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] セントロメアDNAに結合するCENP-T複合体の機能解析2008

    • Author(s)
      堀哲也, ほか
    • Organizer
      BMB
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] Adaptor Nck1 induces increased tyrosine phosphorylation of GIT22008

    • Author(s)
      T Sasaki, ほか
    • Organizer
      BMB
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] mRNA切断活性を示す線虫のArgonaute蛋白についての遺伝生化学的解析2008

    • Author(s)
      田原浩昭, ほか
    • Organizer
      BMB
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] TAP-MS法によるインテグレス結合因子Huwe1の同定とその解析ース結合因子Huwe1の同定とその解析2008

    • Author(s)
      山元誠司, ほか
    • Organizer
      第22回日本エイズ学会学術集会・総会
    • Place of Presentation
      大阪
    • Related Report
      2008 Final Research Report
  • [Presentation] ROLE OF TDRD9 IN MALE MEIOSIS AND REGULATORY PATHWAY OF TRANSPOSON RNA AND DNA METHYLATION2008

    • Author(s)
      M Shoji, ほか
    • Organizer
      6th biennial meeting on Germ Cells
    • Place of Presentation
      CSH
    • Related Report
      2008 Final Research Report
  • [Presentation] AJ fraction を用いた膜タンパク質のプロテオミクス解析2008

    • Author(s)
      山崎裕自, ほか
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] IDENTIFICATION OF CELLULAR INTERACTORS TO MoMLV INTEGRASE USING TANDEM AFFINITY PURIFICATION - MASS SPECTROMETRY ANALYSIS2008

    • Author(s)
      S Yamamoto, ほか
    • Organizer
      33rd annual meeting on Retroviruses
    • Place of Presentation
      CSH
    • Related Report
      2008 Final Research Report
  • [Presentation] Rhoによって制御される細胞質中のForminタンパク質によるアクチン重合2007

    • Author(s)
      東 智仁, ほか
    • Organizer
      BMB2007
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] 血小板における dynaminの発現-アクチン重合への関与の可能性2007

    • Author(s)
      漆原範子, ほか
    • Organizer
      BMB
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] 小胞体関連分解構成因子Derlinsに結合するタンパク質の同定と解析2007

    • Author(s)
      蜷川暁, ほか
    • Organizer
      BMB
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Cell adhesion regulates the interaction between Nck and GIT12007

    • Author(s)
      佐々木知幸, ほか
    • Organizer
      BMB
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Rhoによって制御される細胞質中のForminタンパク質によるアクチン重合2007

    • Author(s)
      東智仁, ほか
    • Organizer
      BMB
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] C. elegans における二次型siRNAの産生と活性についての遺伝生化学的解析2007

    • Author(s)
      田原浩昭, ほか
    • Organizer
      BMB
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://sentan1.hmro.med.kyoto-u.ac.jp/?k-okawa/

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://sentan1.hmro.med.kyoto-u.ac.jp/~k-okawa/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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