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Lipidomic analysis of the action of Phospholipase B/Lipase on intestinal mucosal cell differentiation

Research Project

Project/Area Number 19590307
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionOsaka University

Principal Investigator

TOJO Hiromasa  Osaka University, 生命機能研究科, 准教授 (90135707)

Co-Investigator(Renkei-kenkyūsha) KONDOH Gen  京都大学, 再生医科学研究所, 准教授 (40243258)
Research Collaborator TAKEMURA Hiromasa  大阪大学, 生命機能研究科, 大学院生
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsリピドミクス / ホスホリパーゼB / リパーゼ / 小腸 / 消化吸収 / 分化
Research Abstract

今回新たに作成した小腸刷子縁膜に局在するCa非依存性ホスホリパーゼB/リパーゼ(PLB/LIP)欠損マウス(mPLB-/-)を用いて同酵素の機能解析をした。経口脂質負荷試験では野生型とmPLB-/-で体重・血清脂質値などに差がなかった。回腸Ca^<2+>依存性ホスホリパーゼA2活性が代償的に増加していたことから、PLB/LIPの脂質消化機能を代償する酵素が働いていることが示唆された。最初に作成したmPLB+/-で見られた分化異常はベクター由来のプロモータの影響と考えられた。中性脂質、中性スフィンゴ脂質、リン脂質にわたる自動化脂質一斉分析システムの測定可能脂質クラス範囲を、小腸に多い中性・酸性糖脂質を含む高極性脂質まで拡張できた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2009 2008 2007 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (13 results) Book (4 results)

  • [Journal Article] Cbesity causes a shift in metabolic flow of gangliosides in adipose tissues2009

    • Author(s)
      Tanabe, A., Mgt suds, M, Fukuhara, A., Myata, Y., Komuro, R, Shimomura, I., Tojo, H.
    • Journal Title

      Biochem Biophy.Res.Commun 379

      Pages: 547-552

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Obesity causes a shift in metabolic flow of gangliosides in adipose tissues.2009

    • Author(s)
      Tanabe, A
    • Journal Title

      Biochem. Biophy. Res. Commnun. 379

      Pages: 547-552

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired TCR signaling through dysfunction of lipid rafts in sphingomyelin synthase 1 (SNS1)-knockdown T cells2008

    • Author(s)
      Jin, ZX, Huang, CR, Dong, L., Goda, S., Kawanami T., Sawaki T., Sakai T., Tong XP., Masaki Y., Fukushima T., Tanaka M, Mimori T., Tojo, H., Bloom ET., Ckazaki T., Umehara, H.
    • Journal Title

      Int.Immunol 20

      Pages: 1427-1437

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 2-Hydroxy- ceramide synthesis by ceramide synthase family : enzymatic basis for the preference of FA chain length.2008

    • Author(s)
      Mizutani, Y., Kihara, A., Chiba, H., Tojo, H., Igarashi, Y.
    • Journal Title

      J.Lipid Res. 49

      Pages: 2356-2364

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impaired TCR signaling through dysfunction of lipid rafts in sphingowyelin synthase 1 (SMS1)-knockdown T cells2008

    • Author(s)
      Jin, ZX
    • Journal Title

      Int Immunol. 20

      Pages: 1427-1437

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2-Hydroxy-ceramide synthesis by ceramide synthase family : enzy matic basis for the preference of FA chain length2008

    • Author(s)
      Mizutani, Y.
    • Journal Title

      J Lipid Res. 49

      Pages: 2356-2364

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Characterization of Menbrane-Associated Soluble Serine Palnitoyltransferases from Sphingobacterium multivorun, Sphingobacterium spiritivorun and Bdellovibriostolpii2007

    • Author(s)
      Ikushiro, H., Islam, M., Tojo, H., Hayashi, H.
    • Journal Title

      J.Bactriol 189

      Pages: 5749-5761

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Characterization of Membrane-Associated Soluble Serine Palmitoyltransferases from Sphingobacterium multivorun, Sphingobacterium spiritivorun and Bdellovibrio stolpii.2007

    • Author(s)
      Ikushiro, H
    • Journal Title

      J. Bactriol. 189

      Pages: 5749-5761

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Lipidomics shows that obesity causes a shiftin steady-state metabolic flow of gangliosides downstream from GMB in adipose tissues of murine genetic and nutritional disease models2009

    • Author(s)
      Tojo, H., Tanabe, A.
    • Organizer
      2009 ASMS Sanibel conference
    • Place of Presentation
      フロリダ米国
    • Year and Date
      2009-01-24
    • Related Report
      2008 Final Research Report
  • [Presentation] 遺伝子変異マウスを用いたボスホリパーゼB/リパーゼの生理的機能の解析2008

    • Author(s)
      竹村浩昌, 東城博雅, 近藤玄
    • Organizer
      BMB 2008
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Final Research Report
  • [Presentation] 肥満脂肪組織におけるスフィンゴ糖脂質代謝の変化-HPLC/質量分析による解析2008

    • Author(s)
      田邉文, 福原淳範, 下村伊一郎, 東城博雅
    • Organizer
      BMB 2008
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Final Research Report
  • [Presentation] 遺伝子変異マウスを用いたホスホジパーゼB/リパーゼの生理的機能の解析2008

    • Author(s)
      竹村浩昌
    • Organizer
      BMB2008
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Abeneficial effect of polyphenol oligomers, oligonol, on choline deficiency induced fatty liver in nice2008

    • Author(s)
      Tojo, H. Igura, T., Nbnomura, S., Sumi, R., Bando, Y., Ito. T.
    • Organizer
      49th International Conference on the Bioscience of Lipids
    • Place of Presentation
      マーストリヒト、オランダ
    • Year and Date
      2008-08-29
    • Related Report
      2008 Final Research Report
  • [Presentation] A Beneficial Effect of Polyphenol Oligomers, Oligonol, on Choline deficiency-induced Fatty Liver in mice.2008

    • Author(s)
      Hiromasa Tojo
    • Organizer
      49th International Conference on the Bioscience of Lipids
    • Place of Presentation
      Maastricht University Netherlands
    • Year and Date
      2008-08-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] Sphingolipidomic Profiling of the Stratum Corneum of Mice with Keratinocyte-specific Deletion of Aryl Hydrocarbon Receptor Nuclear Translocator Gene2008

    • Author(s)
      Tojo, H.
    • Organizer
      56th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      デンバー米国
    • Year and Date
      2008-06-04
    • Related Report
      2008 Final Research Report
  • [Presentation] 遺伝子変異マウスを用いたボスホリパーゼB/リパーゼの生理的機能の解析2007

    • Author(s)
      竹村浩昌, 東城博雅, 近藤玄
    • Organizer
      第80会日本生化学大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] 遺伝子変異マウスを用いたホスホリパーゼB/リパーゼの生理的機能の解析2007

    • Author(s)
      竹村 浩昌
    • Organizer
      第80会日本生化学大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] Lipidomic analysis based on normal-phase HPLC/mass spectrometry2007

    • Author(s)
      Tojo, H.
    • Organizer
      5th Euro Fed Lipid Congress
    • Place of Presentation
      ヨーテポリ、スエーデン
    • Year and Date
      2007-09-17
    • Related Report
      2008 Final Research Report
  • [Presentation] Lipidomic analysis based on normal-phase HPLC/mass spectrometry2007

    • Author(s)
      Tojo, H
    • Organizer
      5th Euro Fed Lipid Congress
    • Place of Presentation
      ヨーテボリ、スエーデン
    • Year and Date
      2007-09-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] 48th International Conference on the Bioscience of Lipids Profiling of stratumcorneumlipids by an HPLC/mass spectrometry-based automatedlipidanalyzer2007

    • Author(s)
      Tojo, H.
    • Place of Presentation
      ツルク、ブインランド
    • Year and Date
      2007-09-07
    • Related Report
      2008 Final Research Report
  • [Presentation] Profiling of stratum corneum lipids by an HPLC/mass spectrometry-based automated lipid analyzer2007

    • Author(s)
      Tojo, H
    • Organizer
      48th International Conference on the Bioscience of Lipids
    • Place of Presentation
      ツルク、フィンランド
    • Year and Date
      2007-09-07
    • Related Report
      2007 Annual Research Report
  • [Book] 自動化脂質分析装置を用いた病態リピドミクス"メタポロミクスの先端技術と応用"第20章2008

    • Author(s)
      東城博雅
    • Publisher
      シーエスシー出版
    • Related Report
      2008 Final Research Report
  • [Book] メタボロミクスの先端技術と応用2008

    • Author(s)
      東城 博雅
    • Publisher
      シーエスシー出版
    • Related Report
      2007 Annual Research Report
  • [Book] 質量分析学2008

    • Author(s)
      東城 博雅
    • Publisher
      大阪大学出版会(印刷中)
    • Related Report
      2007 Annual Research Report
  • [Book] リピドミクス質量分析学9章

    • Author(s)
      東城博雅
    • Publisher
      大阪大学出版会(印刷中)
    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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