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The mechanism of regulation of metastasis and alteration in cell matrix by snail, transcription factor induce epithelilal -mesenchmal transduction

Research Project

Project/Area Number 19590313
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKagoshima University

Principal Investigator

HARAGUCHI Misako  Kagoshima University, 大学院・医歯学総合研究科, 准教授 (10244229)

Co-Investigator(Kenkyū-buntansha) OZAWA Masayuki  鹿児島大学, 大学院・医歯学総合研究科, 教授 (90136854)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords分子腫瘍学 / 細胞骨格 / 転移 / snail / E-cadherin / EMT / 接着 / スネイル / インテグリン / 細胞外基質 / ラミニン / オステオポンチン
Research Abstract

We show that the expression of snail in epithelial MDCK and A431 cells enhance both cell detachment and attachment. Snail enhanced cell attachment to ECMs such as fibronectin. Snail also enhanced MDCK cell migration towards osteopontin that are ligands for integrin aVb3. We confirmed the reduction of laminin a3, b3 and g2 (Laminin-5) and of receptorsfor Laminin-5 such as integrins a3, a6 or b4 in MDCK/snail or in A431/snail cells. These results suggest that snail enhances cell detachment by multiple mechanism, and leads cell migration and reattachment at a second site, at least in part, by changing the expression of integrins in the cells.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (10 results)

All 2009 2008 2007

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (5 results)

  • [Journal Article] The role of transcriptional regulator snail in cell detachment, reattachment and migration.2009

    • Author(s)
      *Haraguchi M
    • Journal Title

      Cell adhesion & migratio 3(3)

      Pages: 1-5

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of transcriptional regulator snail in cell detachment, reattachment and migration2009

    • Author(s)
      Haraguchi, M.
    • Journal Title

      Cell adhesion & migration 3

      Pages: 1-5

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of the transcriptional in cell detachment, reattachment and Migration2009

    • Author(s)
      Haraguchi, M.
    • Journal Title

      Adhesion &Migration 3

      Pages: 1-5

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Snail regulates cell-matrix adhesion by regulation of the expression of integrins and basement membrane proteins.2008

    • Author(s)
      *Haraguchi M, Okubo T, Miyashita Y, Miyamoto Y, Hayashi M, Crotti TN, McHugh KP, Ozawa M
    • Journal Title

      J Biol Chem 283(35)

      Pages: 23514-23

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Snail regulates cell-matrix adhesion by regulation of the expression of integrins and basement membrane proteins2008

    • Author(s)
      Haraguchi, M., Okubo, T., Miyashita, Y., et al.
    • Journal Title

      Journal of Biological Chemistry 283

      Pages: 23514-23523

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 転写因子スネイルによるMDCK細胞の代謝調節2009

    • Author(s)
      原口みさ子
    • Organizer
      第82回生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-22
    • Related Report
      2009 Final Research Report
  • [Presentation] 転写因子スネイルによるMDCK細胞の代謝調節2009

    • Author(s)
      原口みさ子
    • Organizer
      第82回日本生化学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] Snail regulates cell-matrix adhesion by regulation of the expression of integrins and basement membrane proteins2008

    • Author(s)
      原口みさ子
    • Organizer
      第67回癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 転写因子スネイルはインテグリンや細胞外マトリックスタンパクの発現を調節することにより細胞と基質間の接着を制御する2007

    • Author(s)
      原口みさ子
    • Organizer
      第80回生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-13
    • Related Report
      2009 Final Research Report
  • [Presentation] The transcription factor Snail regulates the cell-matrix adhesion throughregulating the expression of integrins and extra cellular matrix proteins2007

    • Author(s)
      Haraguchi, M.
    • Organizer
      The 30th Annual Meeting of the Molecular Biology Society of Japan & The 80th Annual Meeting of the Japanese Biochemical Society
    • Place of Presentation
      Yokohama, Japa
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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