Genome-wide analysis of renal cell carcinoma with array-based CGH and transcriptome

• Project/Area Number: 19590355
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Oita University
• Principal Investigator: MATSUURA Keiko Oita University, 医学部, 准教授 (00291542)
• Co-Investigator(Kenkyū-buntansha): MORIYAMA Masatsugu 大分大学, 医学部, 教授 (90239707)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: アレイCGH / 腎癌 / トランスクリプトーム / 遺伝子解析 / 病型分類

Research Abstract

アレイCGHを用いて腎細胞癌(CCC)の発症にかかわるゲノム異常を検索したところ、特に14番染色体長腕(14q)の欠失が、高悪性度のCCCで高頻度にみられた。さらに網羅的遺伝子発現解析(トランスクリプトーム解析)を行い、ゲノムのコピー数異常が遺伝子発現異常に影響を与えていることを見出した。14qで遺伝子発現低下を示した遺伝子のいくつかは、実際に腎癌で遺伝子の発現が減弱しており、CCCの悪性化に関わる癌関連遺伝子と考えられた。

Research Products

• [Journal Article] Genome-wide microRNA expression profiling in renal cell carcinoma : significant downregulation of miR-141 and miR-200c (2008)
  • Author(s): Nakada C., Matsuura K., Tsukamoto Y., Tanigawa M., Yoshimoto T., Narimatsu T., Nguyen LT., Hijiya N., Uchida T., Sato F., Mimata H., Seto M., Moriyama M.
  • Journal Title: The Journal of Pathology 216 Pages: 418-427
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of DNA copy number alterations and gene expression in gastric cancer (2008)
  • Author(s): Tsukamoto Y., Uchida T., Karnan S., Noguchi T., Nguyen LT., Tanigawa M., Takeuchi I., Matsuura K., Hijiya N., Nakada C., Kishida T., Kawahara K., Ito H., Murakami K., Fujioka T., Seto M., Moriyama M.
  • Journal Title: The Journal of Pathology 216 Pages: 471-482
  • Peer Reviewed
• [Journal Article] Phosphorylation status of epidermal growth factor receptor is closely associated with responsiveness to gefitinib in pulmonary adenocarcinoma (2008)
  • Author(s): Hijiya N., Miyawaki M., Kawahara K., Akamine S., Tsuji K., Kadota J., Akizuki S., Uchida T., Matsuura K., Tsukamoto Y., Moriyama M.
  • Journal Title: Human Pathology 39 Pages: 316-323
  • Peer Reviewed
• [Journal Article] Arpp/Ankrd2, a member of the muscle ankyrin repeat proteins (MARPs), translocates from the I-band to the nucleus after muscle injury (2008)
  • Author(s): Tsukamoto Y., Hijiya N., Yano S., Yokoyama S., Nakada C., Uchida T., Matsuura K., Moriyama M.
  • Journal Title: Histochemistry and Cell Biology 129 Pages: 55-64
  • Peer Reviewed
• [Journal Article] Genotyping of the cagA gene of Helicobacter pylori on immunohistochemistry with East Asian CagA-specific antibody (2008)
  • Author(s): Kanada R., Uchida T., Tsukamoto Y., Nguyen LT., Hijiya N., Matsuura K., Kodama M., Okimoto T., Murakami K., Fujioka T., Yanagisawa S., Moriyama M.
  • Journal Title: Pathology International 58 Pages: 218-225
  • Peer Reviewed
• [Journal Article] Genome-wide microRNA expression profiling in renal cell carcinoma : significant downregulation of miR-141 and miR-200c. (2008)
  • Author(s): Nakada C, Matsuura K., Tsukamoto Y., Tanigawa M., Yoshimoto T., Narimatsu T., Nguyen LT., Hijiya N., Uchida T., Sato F., Mimata H., Seto M., Morivama M.
  • Journal Title: The Journal of Pathology 216 Pages: 418-427
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of DNA copy number alterations and gene expression ingastric cancer. (2008)
  • Author(s): Tsukamoto Y., Uchida T., Karnan s., Noguchi T., Nguyen LT., Tanigawa M., Takeuchi I., Matsuura K., Hijiya N., Nakada C., Kishlda T., Kawahara K., Ito H., Murakami K., Fujioka T., Seto M., Moriyama M.
  • Journal Title: The Journal of Pathology 216 Pages: 471-482
  • Peer Reviewed
• [Journal Article] Phosphorylation status of epidermal growth factor receptor is closely associatedwith responsiveness to gefitinib in pulmonary adenocarcinoma. (2008)
  • Author(s): Hijiya N., Miyawaki M., Kawahara K., Akamine S., Tsuji K., Kadota J., Akizuki S., Uchida T., Matsuura K., Tsukamoto Y., Moriyama M
  • Journal Title: Human Pathology 39 Pages: 316-323
  • Peer Reviewed
• [Journal Article] Arpp/Ankrd2, a member of the muscle ankyrin repeat proteins(MARPs), translocates from the I-band to the nucleus after muscle injury. (2008)
  • Author(s): Tsukamoto Y., Hijiya N., Yano S., Yokoyama S., Nakada C, Uchida T., Matsuura K., Morivama M.
  • Journal Title: Histochemistry and Cell Biology 129 Pages: 55-64
  • Peer Reviewed
• [Journal Article] Genotyping of the cagA gene of Helicobacter pylori on immunohistochemistry with East Asian CagA-specific antibody. (2008)
  • Author(s): Kanada R., Uchida T., Tsukamoto Y., Nguyen LT., Hijiya N., Matsuura K., Kodama M., Okimoto T., Murakami K., Fujioka T., Yanagisawa S., Morivama M.
  • Journal Title: Pathology International 58 Pages: 218-225
  • Peer Reviewed
• [Journal Article] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma (2007)
  • Author(s): Yoshimoto T, Matsuura K, Karnan S, Tagawa H, Nakada C, Tanigawa M, Tsukamoto Y, Uchida T, Kashima K, Akizuki S, Takeuchi I, Sato F, Mimata H, Seto M, Moriyama M.
  • Journal Title: Journal of Pathology 213 Pages: 392-401
  • Peer Reviewed
• [Journal Article] Upregulated expression of cardiac ankyrin-repeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis (2007)
  • Author(s): Matsuura K, Uesugi N, Hijiya N, Uchida T and Moriyama M
  • Journal Title: Human Pathology 38 Pages: 410-419
  • Peer Reviewed
• [Journal Article] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma. (2007)
  • Author(s): Yoshimoto T, Matsuura K, Karnan S, Tagawa H, Nakada C, Tanigawa M, Tsukamoto Y, Uchida T, Kashhna K, Akizuki S, Takeuchi I, Sato F, Mimata H, Seto M, Moriyama M.
  • Journal Title: Journal of Pathology 213 Pages: 392-401
  • Peer Reviewed
• [Journal Article] Upregulated expression of cardiac ankyrin-repeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis. (2007)
  • Author(s): Matsuura K, Uesugi N, Hijiya N, Uchida T and Moriyama M
  • Journal Title: Human Pathology 38 Pages: 410-419
  • Peer Reviewed
• [Presentation] High-resolution analysis of DNA copy number alterations and the geneexpression in renal clear cell carcinoma (2008)
  • Author(s): Taichiro Yoshimoto, Keiko Matsuura, Masao Seto, Takahiro Narimatsu, Fuminori Sato, Hiromitsu Mimata, Masatsugu Moriyama
  • Organizer: American Urological Association (AUA) 2008Annual Meeting
  • Place of Presentation: Florida
  • Year and Date: 20080500
• [Presentation] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma (2008)
  • Author(s): Taichiro Yoshimoto
  • Organizer: American Urological Association (AUA) 2008 Annual Meeting
  • Place of Presentation: Florida
  • Year and Date: 2008-05-18
• [Presentation] アレイCGHによる腎癌転移巣における網羅的ゲノム解析 (2008)
  • Author(s): 成松隆弘
  • Organizer: 第96回日本泌尿器科学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-26
• [Presentation] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma (2007)
  • Author(s): Keiko Matsuura
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-04
• [Presentation] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma (2007)
  • Author(s): Keiko Matsuura, Taichiro Yoshimoto, Sivasundaram Karnan, Hiroyuki Tagawa, Chisato Nakada, Masato Tanigawa, Yoshiyuki Tsukamoto, Tomohisa Uchida, Ichiro Takeuchi, Fuminori Sato, Hiromitsu Mimata, Masao Seto, Masatsugu Moriyama
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜市(パシフィコ横浜)
  • Year and Date: 2007-10-04