Abnormality of synovial mesenchymal cells in autoimmune arthritis

• Project/Area Number: 19590390
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kawasaki Medical School
• Principal Investigator: ISHIHARA Katsuhiko Kawasaki Medical School, 教授 (10263245)
• Research Collaborator: IGARASHI Hideya 川崎医科大学, 免疫学, 准教授 (40291538)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 疾患モデル動物 / 滑膜線維芽細胞 / 関節リウマチ / 免疫学 / gp130 / 滑膜繊維芽細胞

Research Abstract

遺伝子改変関節炎発症型マウスの滑膜組織より間葉系細胞株のクローンを樹立した。表面抗原は骨髄間質細胞と類似しており、骨髄細胞からマクロファージ系細胞の発生を支持する機能を有した。これらの細胞株は間葉系細胞、滑膜線維芽細胞、骨芽細胞に特徴的な遺伝子を発現していた。HTLV-1のpX遺伝子を過剰発現するマウス由来の滑膜線維芽細胞株では、破骨細胞分化誘導能が亢進していた。これらの滑膜線維芽細胞に関する基礎的解析結果は、関節リウマチの病態の理解につながる重要な知見を提供した。

Research Products

• [Journal Article] Point mutation of tyrosine 759 of the IL-6 family cytokine receptor, gp130, augments collagen-induced arthritis in DBA/1J mice (2009)
  • Author(s): Tsuji F, Yoshimi M, Katsura O, Takai M, Ishihara k, Aono H.
  • Journal Title: BMC Musculo -skelet Dissord 10 Pages: 23-23
  • Peer Reviewed
• [Journal Article] Point mutation of tyrosine 759 of the IL-6 femily eytokine receptor. gp130, augments collagen-induced arthritis in DBA/1J mice. (2009)
  • Author(s): Fumio Tsuji, Katsuhiko Ishihara, Hiroyuki Aono, et al.
  • Journal Title: BMC Musculoskeltal disorders 10 Pages: 23-32
  • Peer Reviewed
• [Journal Article] Interleukin-6 directly inhibits osteoclast differentiation by suppressing receptor activation of NF-kappa B signaling pathway (2008)
  • Author(s): Ebis S.
  • Journal Title: J.Biol.Chem. 238 Pages: 11535-11540
  • Peer Reviewed
• [Journal Article] Interleukin-6 directly inhibits osteoclast differentiation by suppressing receptor activator of NF-κB signaling pathways. (2008)
  • Author(s): Fumio Yoshitake, Shousaku Itoh, Katsuhiko Ishihara, et al.
  • Journal Title: Journal of biological chemistry 283 Pages: 11535-11540
  • Peer Reviewed
• [Journal Article] サイトカインとは (2008)
  • Author(s): 石原克彦
  • Journal Title: 小児科診療 71 Pages: 2093-2102
• [Presentation] Search for cell-intrinsic abnormalities of synovial fibroblasts in rheumatoid arthritis (2007)
  • Author(s): Katsuhiko Ishihara, Hideya Igarashi, Toshiyuki Fukada, Masaaki Murakami and Toshio Hirano
  • Organizer: 第37回日本免疫学会学術集会
  • Place of Presentation: 東京