Identification and purification of sympathetic nerve neural crest stem cells and neuroblastoma cancer stem cells

• Project/Area Number: 19590409
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Aichi Medical University
• Principal Investigator: KUROKAWA Kei Aichi Medical University, 医学部, 講師 (90399030)
• Co-Investigator(Kenkyū-buntansha): IWASHITA Toshihide 愛知医科大学, 医学部, 准教授 (00283432)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 腫瘍 / 癌幹細胞 / 神経芽細胞腫 / 交感神経神経堤幹細胞

Research Abstract

ラット新生児副腎髄質より神経堤幹細胞の分離を試み、p75陽性分画に神経細胞とシュワン細胞の両方向性分化能を示すコロニー形成を確認した。p75発現を認める神経芽腫細胞株で、p75陽性分画が分化階層の上位にあることを細胞培養レベルで確認した。神経芽腫細胞株を用いたスクリーニングで、神経芽腫cancer stem cellや交感神経系神経堤幹細胞の細胞表面マーカー候補となりうる分子についてのデータを得た。

Research Products

• [Journal Article] Pleomorphic carcinoma of the breast with osteoclastic giant cells (2009)
  • Author(s): Kurokawa K, Mouri Y, Asano A, Kamei K, Iwata Y, Isogai M, Saga S, Ichihara S.
  • Journal Title: Pathol Int 59 Pages: 91-97
  • Peer Reviewed
• [Journal Article] Bmi-1 over-expression in neural stem/progenitor cells increases proliferation and neurogenesis in culture but has little effect on these functions in vivo. (2009)
  • Author(s): He S, Iwashita T, Buchstaller J, Molofsky AV, Thomas D, Morrison SJ.
  • Journal Title: Dev Biol 328 Pages: 257-272
  • Peer Reviewed
• [Journal Article] Galanin receptor subtype 2 suppresses cell proliferation and induces apoptosis in p53 mutant head and neck cancer cells (2009)
  • Author(s): Kanazawa T, Kommareddi PK, Iwashita T, Kumar B, Misawa K, Misawa Y, Jang I, Nair TS, Iino Y, Carey TE.
  • Journal Title: Clin Cancer Res 15 Pages: 2222-2230
  • Peer Reviewed
• [Journal Article] Increased expression of glial cell line-derived neurotrophic factor and neurturin in a case of colon adenocarcinoma associated with diffuse ganglioneuromatosis (2009)
  • Author(s): Qiao S, Iwashita T, Ichihara M, Murakumo Y, Yamaguchi A, Isogai M, Sakata K, Takahashi M.
  • Journal Title: Clin Neuropathol 28 Pages: 105-112
  • Peer Reviewed
• [Journal Article] Pleomorphic carcinoma with osteoclastic giant cells of the breast (2009)
  • Author(s): Kurokawa K, et al.
  • Journal Title: Pathology International 59 Pages: 91-97
  • Peer Reviewed
• [Journal Article] Galanin and Galanin Receptor Type 1 Suppress Proliferation in Squamous Carcinoma Cells : Activation of the Extracellular Signal Regulated Kinase Pathway and Induction of Cyclin-dependent Kinase Inhibitors (2007)
  • Author(s): Kanazawa T, Iwashita T, Kommareddi P, Nair T, Misawa K, Misawa Y, Ueda Y, Tono T, Carey TE.
  • Journal Title: Oncogene 26 Pages: 5762-5771
  • Peer Reviewed
• [Journal Article] Analysis of DOK-6 function in downstream signaling of RET in human neuroblastoma cells
  • Author(s): Kurotsuchi A, Murakumo Y, Jijiwa M, Kurokawa K, Itoh Y, Kodama Y, Kato T, Enomoto A, Asai N, Terasaki H, Takahashi M.
  • Journal Title: Cancer Sci (in press)
  • Peer Reviewed
• [Presentation] Ratグリオーマ組織内に存在する bipotent な分化能を有する細胞の同定 (2009)
  • Author(s): 黒川景, 喬善楼, 川井久美, 佐賀信介, 岩下寿秀
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-01
• [Presentation] オリゴデンドロサイトの発生制御におけるBAX/BAKの役割の解析 (2009)
  • Author(s): 川井久美, 伊藤高行, 黒川景, 岩下寿秀, 佐賀信介
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-01
• [Presentation] ラットグリオーマ組織内におけるSP (side-population) 細胞の局在 (2008)
  • Author(s): 黒川景, 喬善楼, 佐賀信介, 岩下寿秀
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] ラットグリオーマ組織内におけるSP(side-population)細胞の局在 (2008)
  • Author(s): 黒川景、喬善楼、佐賀信介、岩下寿秀
  • Organizer: 第97回 日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17