|Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
We observed invading amastigote of Leishmania amazonensisto macrophage host cells with microscope, and performed the temporary changes of the host cell with cDNA microarray. Total RNA was separated from the non-infected host cell which a mouse macrophage cell strain (J774A.1), infected cells in early stages of protozoan infection, in the infectious formation period, and parasites. Those samples were analyzed about 41,252 probes using the Oligo-microarray system (Agilent Technologies) (4 X 44K) 1 color method.