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Partial genomic analysis of uncultured Anaplasma phagocytophilum in Japan and the development for a simple and rapid diagnostic tool

Research Project

Project/Area Number 19590451
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionUniversity of Shizuoka

Principal Investigator

OHASHI Norio  University of Shizuoka, 食品栄養科学部, 教授 (10169039)

Co-Investigator(Kenkyū-buntansha) MASUZAWA Toshiyuki  千葉科学大学, 薬学部, 教授 (10181645)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords遺伝子 / 感染症 / ゲノム / 細菌 / 微生物
Research Abstract

本研究では、我が国の難培養性アナプラズマ症起因細菌について、媒介マダニの唾液腺中に存在するこの細菌のゲノム部分構造を明らかにすることを目的とし、アナプラズマ細菌に特異的なp44/msp2 主要外被膜蛋白遺伝子群の発現領域の解析を行った。その結果、3 匹のマダニの唾液腺DNA からp44/msp2 発現領域(3-4 kb)の増幅に成功し、その遺伝子構造を明らかにすることに成功した。さらに、リアルタイムPCR による簡易検出法について検討した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (33 results)

All 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (21 results)

  • [Journal Article] Characterization of p44/msp2 multigene family of Anaplasma phagocytophilum from two different ticks species2009

    • Author(s)
      Wuritu, Goawa, F. Kawamori, M. Aochi, T. Masuda, and N. Ohashi
    • Journal Title

      Ixodes persulcatus and Ixodes ovatus, in Japan. Jpn. J. Infect. Dis 62

      Pages: 142-145

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of p44/msp2 multigene family of Anaplasma phagocytophilum from two different ticks species, Ixodes persulcatus and Ixodes ovatus, in Japan.2009

    • Author(s)
      Wuritu, Goawa, F. Kawamori, M. Aochi, T. Masuda, N. Ohashi
    • Journal Title

      Jpn. J. Infect. Dis. 62(2)

      Pages: 142-145

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and genetic diversity of Bartonella species isolated from wild rodents in Japan2008

    • Author(s)
      K. Inoue, S. Maruyama, H. Kabeya, N. Yamada, N. Ohashi, Y. Sato, M. Yukawa, T. Masuzawa, F. Kawamori, T. Kadosaka, N..Takada, H. Fujita, and H. Kawabata
    • Journal Title

      Appl. Environ. Microbiol 74

      Pages: 5086-5092

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analyses of neutrophil-like differentiated cell lines under a hyperglycemic condition2008

    • Author(s)
      T. Oya-Ito, H. Naitou, S. Masuda, N. Kinae, N. Ohashi
    • Journal Title

      Mol. Nutr. Food Res 52

      Pages: 360-369

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Anaplasma phagocytophilum and its coinfection with Borrelia afzelii in Ixodes ricinus and Ixodes persulcatus ticks inhabiting Tver's Province (Russia) -a sympatric region for the two tick species2008

    • Author(s)
      T. Masuzawa, I.G. Kharitonenkov, Y. Okamoto, T. Fukui and N. Ohashi
    • Journal Title

      J. Med. Microbiol 57

      Pages: 986-991

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular typing of Japanese Escherichia coli O157:H7 isolates from clinical specimens by multilocus variable-number tandem repeat analysis and PFGE2008

    • Author(s)
      F. Kawamori, M. Hiroi, T. Harada, K. Ohata, K. Sugiyama, T Masuda, and N. Ohashi
    • Journal Title

      J. Med. Microbiol 57

      Pages: 58-63

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and genetic diversity of Bartonella species isolated from wild rodents in Japan.2008

    • Author(s)
      K. Inoue, S. Maruyama, H. Kabeya, N. Yamada, N. Ohashi, et al.
    • Journal Title

      Appl. Environ. Microbiol. 74

      Pages: 5086-5092

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analyses of neutrophil-like differentiated cell lines under a hyperglycemic condition.2008

    • Author(s)
      T. Oya-Ito, H. Naitou, S. Masuda, N. Kinae, N. Ohashi
    • Journal Title

      Mol. Nutr. Food Res. 52

      Pages: 360-369

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Anaplasma phagocytophilum and its coinfection with Borrelia afzelii in Ixodes ricinus and Ixodes persulcatus ticks inhabiting Tver's Province(Russia)-a sympatric region for the two tick species.2008

    • Author(s)
      T. Masuzawa. I. G. Kharitonenko v, Y. Okamoto, T. Fukui, N. Ohashi
    • Journal Title

      J. Med. Microbiol. 57

      Pages: 986-991

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular typing of Japanese Escherichia coli O157 : H7 isolates from clinical specimens by multilocus variable-number tandem repeat analysis and PFGE.2008

    • Author(s)
      F. Kawamori, M. Hiroi, T. Harada, K. Ohata, K. Sugiyama, T Masuda, N. Ohashi
    • Journal Title

      J. Med. Microbiol. 57

      Pages: 58-63

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular typing of Japanese Escherichia coli O157:H7 isolates from clinical specimens by multilocus variable-number tandem repeat analysis and PFGE2008

    • Author(s)
      F. Kawamori, M. Hiroi, T. Harada, K. Ohata, K. Sugiyama, T. Masuda, and N. Ohashi
    • Journal Title

      J. Med. Microbiol. 57

      Pages: 58-63

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Anaplasma phagocytophilum and its coinfection with Borrelia afzelii in lxodes ricinus and lxodes persulcatus ticks inhabiting Tver's Province(Russia)-a sympatric region for the two tick species

    • Author(s)
      T. Masuzawa, I. G. Kharitonenkov, Y. Okamoto, T. Fukui and N. Ohashi
    • Journal Title

      J. Med. Microbiol. (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 国内初の新興感染症「アナプラズマ症」について2009

    • Author(s)
      大橋典男
    • Organizer
      第82 回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Final Research Report
  • [Presentation] 静岡県東部で採取されたマダニにおける紅斑熱群リケッチア検出状況2009

    • Author(s)
      川森文彦
    • Organizer
      第82 回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Final Research Report
  • [Presentation] 紀伊半島のマダニから検出された紅斑熱群リケッチアDNAの解析2009

    • Author(s)
      青地美南
    • Organizer
      第82 回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Final Research Report
  • [Presentation] 紀伊半島のマダニから検出された紅斑熱群リケッチアDNAの解析2009

    • Author(s)
      青地美南, 他
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] 静岡県東部で採取されたマダニにおける紅斑熱群リケッチア検出状況2009

    • Author(s)
      川森文彦, 他
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] 国内初の新興感染症「アナプラズマ症」について2009

    • Author(s)
      大橋典男, 他
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] 鹿児島県のマダニが保有するリケッチア関連細菌群について2008

    • Author(s)
      高娃
    • Organizer
      平成20年度日本薬学会東海支部例会
    • Place of Presentation
      静岡
    • Year and Date
      2008-12-06
    • Related Report
      2008 Final Research Report
  • [Presentation] 鹿児島県のマダニが保有するリケッチア関連細菌群について2008

    • Author(s)
      高娃, 他
    • Organizer
      平成20年度日本薬学会東海支部例会
    • Place of Presentation
      静岡市
    • Year and Date
      2008-12-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] マダニ唾液腺内に存在するアナプラズマ症起因細菌のゲノム部分構造解析へのアプローチ2008

    • Author(s)
      大橋典男
    • Organizer
      第26 回日本クラミジア研究会・第15 回リケッチア研究会・合同研究発表会
    • Place of Presentation
      岐阜
    • Year and Date
      2008-11-02
    • Related Report
      2008 Final Research Report
  • [Presentation] 鹿児島県および長崎県五島列島のマダニが保有するアナプラズマ・エーリキア・リケッチアについて2008

    • Author(s)
      高娃
    • Organizer
      第26回日本クラミジア研究会・第15 回リケッチア研究会・合同研究発表会
    • Place of Presentation
      岐阜
    • Year and Date
      2008-11-02
    • Related Report
      2008 Final Research Report
  • [Presentation] Multiplex real-time RT-PCRによるつつが虫病リケッチアおよび紅斑熱群リケッチアの検出2008

    • Author(s)
      川森文彦
    • Organizer
      第26 回日本クラミジア研究会・第15 回リケッチア研究会・合同研究発表会
    • Place of Presentation
      岐阜
    • Year and Date
      2008-11-02
    • Related Report
      2008 Final Research Report
  • [Presentation] 新興感染症「アナプラズマ症」患者の発見2008

    • Author(s)
      大橋典男
    • Organizer
      第26 回日本クラミジア研究会・第15 回リケッチア研究会・合同研究発表会
    • Place of Presentation
      岐阜
    • Year and Date
      2008-11-02
    • Related Report
      2008 Final Research Report
  • [Presentation] 新興感染症「アナプラズマ症」患者の発見2008

    • Author(s)
      大橋典男, 他
    • Organizer
      第26回日本クラミジア研究会・第15回リケッチア研究会・合同研究発表会
    • Place of Presentation
      岐阜市
    • Year and Date
      2008-11-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] Multiplex real-time RT-PCRによるつつが虫病リケッチアおよび紅斑熱群リケッチアの検出2008

    • Author(s)
      川森文彦, 他
    • Organizer
      第26回日本クラミジア研究会・第15回リケッチア研究会・合同研究発表会
    • Place of Presentation
      岐阜市
    • Year and Date
      2008-11-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] 鹿児島県および長崎県五島列島のマダニが保有するアナプラズマ・エージキア・リケッチアについて2008

    • Author(s)
      高娃, 他
    • Organizer
      第26回日本クラミジア研究会・第15回リケッチア研究会・合同研究発表会
    • Place of Presentation
      岐阜市
    • Year and Date
      2008-11-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] マダニ唾液腺内に存在するアナプラズマ症起因細菌のゲノム部分構造解析へのアプローチ2008

    • Author(s)
      大橋典男, 他
    • Organizer
      第26回日本クラミジア研究会・第15回リケッチア研究会・合同研究発表会
    • Place of Presentation
      岐阜市
    • Year and Date
      2008-11-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] Characterization of rickettsial DNA (spotted fever group) from ticks collected in Kagoshima prefecture, Japan2008

    • Author(s)
      N. Ohashi
    • Organizer
      The International Crisis Management Symposium on CBRN and Emerging Infectious Diseases
    • Place of Presentation
      Choshi
    • Year and Date
      2008-09-14
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of rickettsial DNA (spotted fever group)from ticks collected in Kagoshima prefecture. Japan2008

    • Author(s)
      N. Ohashi, et al.
    • Organizer
      The International Crisis Management Symposium on CBRN and Emerging Infectious Diseases
    • Place of Presentation
      銚子市
    • Year and Date
      2008-09-14
    • Related Report
      2008 Annual Research Report
  • [Presentation]2008

    • Author(s)
      大橋典男
    • Organizer
      第81 回日本細菌学会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-26
    • Related Report
      2008 Final Research Report
  • [Presentation] 鹿児島県のマダニから検出された紅斑熱リケッチアDNAの解析2008

    • Author(s)
      大橋 典男、高娃、鳥日図、川森文彦
    • Organizer
      第81回 日本細菌学会総会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] 鹿児島県のマダニから検出された紅斑熱群リケッチアの遺伝子解析2007

    • Author(s)
      大橋典男
    • Organizer
      第25 回日本クラミジア研究会・第14 回リケッチア研究会・合同研究発表会
    • Place of Presentation
      東京
    • Year and Date
      2007-10-27
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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