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Realtime analysis of influenza virus kinetics

Research Project

Project/Area Number 19590468
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionThe University of Tokyo

Principal Investigator

IWATSUKI-HORIMOTO Kiyoko  The University of Tokyo, 医科学研究所, 特任助教 (20376619)

Co-Investigator(Kenkyū-buntansha) HORIMOTO Taisuke  東京大学, 医科学研究所, 准教授 (00222282)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細胞 / インフルエンザ / 細胞内輸送 / 蛍光標識
Research Abstract

The NS2 protein of influenza A virus is thought to be required for nuclear export of viral ribonucleoprotein complexes. To determine the kinetics of the NS2 protein during viral replication, we introduced full-length Venus to the N terminus of the NS cDNA encoding the NS2 protein and generated Venus-tagged NS2 mutant virus by reverse genetics. Madin-Darby Canine Kidney cells were infected with the Venus-tagged NS2 mutant virus, and monitored by time-laps. Then we got dynamics image of the NS2 protein in a cell. NS2 protein was accumulated in the nucleus at the first stage of the infection and then burst to the cytoplasm. This Venus-tagged NS2 mutant virus will likely be useful for analysis of subcellular trafficking of the NS2 protein.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (39 results)

All 2010 2009 2008 2007 Other

All Journal Article (28 results) (of which Peer Reviewed: 17 results) Presentation (7 results) Book (3 results) Remarks (1 results)

  • [Journal Article] 新型インフルエンザとは何か?2010

    • Author(s)
      岩附研子, 河岡義裕
    • Journal Title

      消防科学と情報 99

      Pages: 13-17

    • Related Report
      2009 Final Research Report
  • [Journal Article] 新型インフルエンザとは何か?2010

    • Author(s)
      岩附(堀本)研子, 河岡義裕
    • Journal Title

      消防科学と情報 99

      Pages: 13-17

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro and in vivo characterization of new swine-origin HINT influenza viruses2009

    • Author(s)
      Itoh Y, Shinya K, Kiso M, Watanabe T, Sakoda Y, Hatta M, Muramoto Y, Tamura D, Sakai-Tagawa Y, Noda T, Sakabe S, Imai M, Hatta Y, Watanabe S, Li C, Yamada S, Fujii K, Murakami S, Imai H, Kakugawa S, Ito M, Takano R, Iwatsuki-Horimoto K, Shimojima M, Horimoto T, Goto H, Takahashi K, Makino A, Ishigaki H, Nakayama M, Okamatsu M, Takahashi K, Warshauer D, Shult PA, Saito R, Suzuki H, Furuta Y, Yamashita M, Mitamura K, Nakano K, Nakamura M, Brockman-Schneider R, Mitamura H, Yamazaki M, Sugaya N, Suresh M, Ozawa M, Meumann G.Gem J, Kida H, Ogawsawara K, Kawaoka Y.
    • Journal Title

      Nature 460

      Pages: 1021-1025

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Incorporation of influenza A virus genome segments does not absolutely require wild-type sequences2009

    • Author(s)
      Fujii K, Ozawa M, Iwatsuki-Horimoto K, Horimoto T, Kawaoka Y.
    • Journal Title

      Journal of General Virology 90

      Pages: 1734-1740

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nucleotide sequence requirements at the 5' end of the influenza A virus M RNA segment for efficient virus replication2009

    • Author(s)
      Ozawa M, Maeda J, Iwatsuki-Horimoto K, Watanabe S, Goto H, Horimoto T, Kawaoka Y.
    • Journal Title

      Journal of Virology 83

      Pages: 3384-3388

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] H5N1ウイルス感染患者の病態2009

    • Author(s)
      坂部沙織, 岩附研子, 河岡義裕
    • Journal Title

      Medical Bio 39

      Pages: 47-49

    • Related Report
      2009 Final Research Report
  • [Journal Article] Nucleotide sequence requirements at the 5' end of the influenza A virus MRNA segment for efficient virus replication2009

    • Author(s)
      Ozawa M, Maeda J, 岩附(堀本)研子, et al.
    • Journal Title

      Journal of Virology 83

      Pages: 3384-3388

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Incorporation of influenza A virus genome segments does not absolutely require wild-type sequences2009

    • Author(s)
      Fujii K, Ozawa M, 岩附(堀本)研子, et al.
    • Journal Title

      Journal of General Virology 90

      Pages: 1734-1740

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses.2009

    • Author(s)
      Itoh Y, Shinya K, Kiso M, Watanabe T, et al.
    • Journal Title

      Nature 460

      Pages: 1021-1025

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] H5N1ウイルス感染患者の病態2009

    • Author(s)
      坂部沙織、岩附研子、河岡義裕
    • Journal Title

      Medical Bio 39

      Pages: 47-494

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Cross-clade protective immunity of H5N1 influenza vaccines in a mouse model2008

    • Author(s)
      Murakami S, Iwasa A, Iwatsuki-Horimoto K, Ito M, Kiso M, Kida H, Takada A, Nidom CA, Le MQ, Yamada S, Imai H, Sakai-Tagawa Y, Kawaoka Y, Horimoto T.
    • Journal Title

      Vaccine 26

      Pages: 6398-6404

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Growth determinants for H5N1 influenza vaccine seed viruses in MDCK cells2008

    • Author(s)
      Murakami S, Horimoto T, Le MQ, Midom CA, Chen H, Muramoto T, Yamada S, Iwasa A, Iwatsuki-Horimoto K, Shimojima M, Iwata A, Kawaoka Y.
    • Journal Title

      Journal of Virology 82

      Pages: 10502-10509

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Limited compatibility between the RNA polymerase components of influenza virus type A and B.2008

    • Author(s)
      Iwatsuki-Horimoto, K., Hatta, Y., Hatta, M., Muramoto, Y., Chen, H., Kawaoka, Y., Horimoto, T.
    • Journal Title

      Virus Research 135

      Pages: 161-165

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] What are new influenza viruses?2008

    • Author(s)
      Iwatsuki-Horimoto K, Kawaoka Y.
    • Journal Title

      Medical Science Digest 34

      Pages: 614-617

    • Related Report
      2009 Final Research Report
  • [Journal Article] 新型インフルエンザ2008

    • Author(s)
      岩附研子, 河岡義裕
    • Journal Title

      からだの科学 259

      Pages: 101-105

    • Related Report
      2009 Final Research Report
  • [Journal Article] 鳥インフルエンザから新型インフルエンザへ2008

    • Author(s)
      岩附研子, 堀本泰介, 河岡義裕
    • Journal Title

      臨床と研究 85

      Pages: 1681-1686

    • Related Report
      2009 Final Research Report
  • [Journal Article] Limited compatibility between the RNA polymerase components of infiuenza virus type A and B.2008

    • Author(s)
      Iwatsuki-Horimoto. K., Hatta, Y., Hatta,M., et al
    • Journal Title

      Virus Research 135

      Pages: 161-165

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth determihants for H5N1 influenza vaccine seed viruses in MDCK cells2008

    • Author(s)
      Murakami S, Horimoto T, Le MQ, et al
    • Journal Title

      Journal of Virology 82

      Pages: 10502-10509

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cross-clade protective immunity of H5N1 influenza vaccines in amouse model.2008

    • Author(s)
      Murakami S, Iwasa A, Iwatsuki-Horimoto K, et al.
    • Journal Title

      Vaccine 26

      Pages: 6398-6404

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新型インフルェシザ2008

    • Author(s)
      岩附研子、河岡義裕
    • Journal Title

      からだの科学 259

      Pages: 101-105

    • Related Report
      2008 Annual Research Report
  • [Journal Article] "What are new influenza Viruses?"2008

    • Author(s)
      IWatsuki-Horimoto K, Kawaoka Y.
    • Journal Title

      Medical Science Digest 34

      Pages: 614-617

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 鳥インフルエンザから新型インフルエンザヘ2008

    • Author(s)
      岩附研子、堀本泰介、河岡義裕
    • Journal Title

      臨床と研究 85

      Pages: 1681-1686

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Anovel M-cell specific carbohydrate-targeted mucosal vaccine effectively induces antigen-specific immune responses2007

    • Author(s)
      Nochi, T., Yuki, Y., Matsumura, A., Mejima, M., Terahara, K., Kim, D., Fukuyama, S., Iwatsuki-Horimoto, K., Kawaoka, Y., Kohda, T., Kozaki, S., Igarashi, O., Kiyono, H.
    • Journal Title

      J. Exp. Med. 204

      Pages: 2789-2796

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced growth of seed viruses for H5N1 influenza vaccines2007

    • Author(s)
      Horimoto, T., Murakami, S., Muramoto Y., Yamada, S., Fujii, K., Kiso, M., Iwatsuki-Horimoto, K., Kino, Y., Kawaoka, Y.
    • Journal Title

      Virology 366

      Pages: 23-27

    • Related Report
      2009 Final Research Report
  • [Journal Article] Enhanced growth of seed virus for H5N1 influenza vaccine.2007

    • Author(s)
      Horimoto T., et. al.
    • Journal Title

      Virology 366

      Pages: 23-27

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel M-cell specific carbohydrate-targeted mucosal vaccine effectivelyinduces antigen-specific immune responses.2007

    • Author(s)
      Nochi T., et. al.
    • Journal Title

      J. Exp. Med. 204

      Pages: 2789-2796

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 100 years since "Spanish Flu": What have we learned?2007

    • Author(s)
      Iwatsuki-horimoto K. and Kawaoka Y
    • Journal Title

      Prog. Med. 27

      Pages: 2259-2264

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Significance of seasonal influenza viruses in the stool of pediatric patients

    • Author(s)
      Tamura D, Fujino M, Ozawa M, Iwatsuki-Horimoto K, Goto H, Sakai-Tagawa Y, Horimoto T, Nirasawa M, Kawaoka Y.
    • Journal Title

      The Pediatric Infectious Disease Journal (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] 新型インフルレエンザとは何か? インフルエンザウイルスによるパンデミックの脅威2010

    • Author(s)
      岩附研子
    • Organizer
      第239回クリーンテクノロジー研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-03
    • Related Report
      2009 Final Research Report
  • [Presentation] 新型インフルエンザとは何か?インフルエンザウイルスによるパンデミックの脅威2010

    • Author(s)
      岩附研子
    • Organizer
      第239回クリーンテクノロジー研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] NS1/NS2 mutations influence influenza A virus morphology and virulence2008

    • Author(s)
      Iwatsuki-Horimoto K.
    • Organizer
      XIVth International Congress of Virology
    • Place of Presentation
      イスタンブール
    • Year and Date
      2008-08-12
    • Related Report
      2009 Final Research Report
  • [Presentation] NS1/NS2 mutations influence influenza A virus morphology and virulence.2008

    • Author(s)
      Iwatsuki-Horimoto K
    • Organizer
      XIVth International Congress of virology
    • Place of Presentation
      イスタンブール
    • Year and Date
      2008-08-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] A型とB型インフルエンザウイルスのポリメラーゼサブユニットの互換性2007

    • Author(s)
      岩附研子
    • Organizer
      第30回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Related Report
      2009 Final Research Report
  • [Presentation] A型とB型インフルエンザウイルスのポリメラーゼサブユニット間の互換性2007

    • Author(s)
      岩附 研子
    • Organizer
      第30回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] B型インフルエンザウイルスのポリメラーゼはA型ウイルスゲノムのプロモーターを認識しうるか?2007

    • Author(s)
      岩附研子
    • Organizer
      第55回日本ウイルス学会総会
    • Place of Presentation
      札幌
    • Year and Date
      2007-10-22
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Book] 人がつなげる科学の歴史(1)『ワクチンと薬の発見』(キャロル・バラード著、西川美樹訳, 日本語監修)2010

    • Author(s)
      堀本泰介, 堀本研子
    • Publisher
      文漢堂
    • Related Report
      2009 Final Research Report
  • [Book] インフルエンザパンデミック(ブルーバックス)2009

    • Author(s)
      河岡義裕, 堀本研子
    • Publisher
      講談社
    • Related Report
      2009 Final Research Report
  • [Book] インフルエンザ パンデミック2009

    • Author(s)
      河岡義裕, 堀本(岩附)研子
    • Total Pages
      238
    • Publisher
      講談社
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ims.u-tokyo.ac.jp/virology/index.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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