Molecular mechanisms of lymphocyte migration to the skin and its regulation

• Project/Area Number: 19590496
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: HIRATRA Takako Osaka University, 微生物病研究所, 特任准教授 (00346199)
• Co-Investigator(Kenkyū-buntansha): SHIGETA Akiko 大阪大学, 微生物病研究所, 特任研究員 (50420435)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: T細胞 / 接着分子 / セレクチン / シアロムチン / CD43 / PSGL-1 / 皮膚 / 白血球ローリング

Research Abstract

体の外面を覆う皮膚に浸潤するT 細胞は感染防御において中心的役割を担うだけでなく、アトピー性皮膚炎などの炎症性皮膚疾患の病因・病態にも関与する。その浸潤は血管内皮細胞上のセレクチンとT 細胞上のセレクチンリガンドとの相互作用により開始されるが、その分子レベルでの詳細は不明であった。本研究では、T 細胞上のセレクチンリガンドとしてPSGL-1、CD43、ESL-1 を同定し、これらの分子が協同的にT 細胞の皮膚浸潤を媒介することを明らかにした

Research Products

• [Journal Article] An L-selectin ligand distinct from Pselectinglycoprotein ligand-1 is expressed onendothelial cells and promotes neutrophilrolling in inflammation. (2008)
  • Author(s): Shigeta, A., Matsumoto, M., Tedder, T.F., Lowe, L.B., Miyasaka, M., and Hirata, T
  • Journal Title: Blood 112 Pages: 4915-4923
  • Peer Reviewed
• [Journal Article] T 細胞の炎症局所への動員におけるCD43 の生理的役割 (2008)
  • Author(s): 松本真典, 平田多佳子
  • Journal Title: 臨床免疫・アレルギー科 442
  • NAID: 40016324085
• [Journal Article] Nepmucin/CLM-9, an Ig domain-containingsialomucin in vascular endothelial cells, promotes lymphocyte transendothelialmigration in vitro (2008)
  • Author(s): Jin, S., Umemoto, E., Tanaka, T., Shimomura, Y., Tohya, K., Kunizawa, K., Yang, B.G., Jang, M.H., Hirata, T., and Miyasaka, M.
  • Journal Title: FEBS Lett 582 Pages: 3018-3024
  • Peer Reviewed
• [Journal Article] CD43 plays bothantiadhesive and proadhesive roles inneutrophil trafficking in a context-dependentmanner (2008)
  • Author(s): Matsumoto, M., Shigeta, A., Miyasaka, M., and Hirata, T
  • Journal Title: J. Immunol 181 Pages: 3628-3635
  • Peer Reviewed
• [Journal Article] Chondroitinsulfate E fragments enhance CD44 cleavageand CD44-dependent motility in tumor cells. (2008)
  • Author(s): Sugahara, K.N., Hirata, T., Tanaka, T., Ogino, S., Takeda, M., Terasawa H., Shimada, I., Tamura, J., ten Dam, G.B., van Kuppevelt, T.H., and Miyasaka, M
  • Journal Title: Cancer Res 68 Pages: 7191-7199
  • Peer Reviewed
• [Journal Article] Identification of novel isoforms ofmouse L-selectin with different carboxyterminaltails (2008)
  • Author(s): Furukawa, Y., Umemoto, E., Jang, M.H., Tohya, K., Miyasaka, M., and Hirata, T
  • Journal Title: J. Biol. Chem 283 Pages: 12112-12119
  • Peer Reviewed
• [Journal Article] CD4+CD25+ regulatory T cells in the smallintestinal lamina propria show aneffector/memory phenotype (2008)
  • Author(s): Guo, Z., Jang, M.H., Otani, K., Bai, Z., Umemoto, E., Matsumoto, M., Nishiyama, M., Yamasaki, M., Ueha, S., Matsushima, K., Hirata, T., and Miyasaka, M
  • Journal Title: Int. Immunol 20 Pages: 307-315
  • Peer Reviewed
• [Journal Article] Identification of novel isoforms of mouse L-selectin with different carboxy-terminal tails (2008)
  • Author(s): Furukawa, Y.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 12112-1211
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate E fragments enhance CD44 cleavage and CD44-dependent motility in tumor cells (2008)
  • Author(s): Sugahara, K. N.
  • Journal Title: Cancer Research 68 Pages: 7191-7199
  • Peer Reviewed
• [Journal Article] CD43 plays both antiadhesive and proadhesive roles in neutrophil trafficking in a context-dependent manner (2008)
  • Author(s): Matsumoto, M.
  • Journal Title: Journal of Immunology 181 Pages: 3628-3635
  • Peer Reviewed
• [Journal Article] Nepmucin/CLM-9, an Ig domain-containing sialomucin in vascular endothelial cells, promotes lymphocyte transendothelial migration in vitro (2008)
  • Author(s): Jin, S.
  • Journal Title: FEBS Letters 582 Pages: 3018-3024
  • Peer Reviewed
• [Journal Article] An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation (2008)
  • Author(s): Shigeta, A.
  • Journal Title: Journal of Immunology 112 Pages: 4915-4923
  • Peer Reviewed
• [Journal Article] T細胞の炎症局所への動員におけるCD43の生理的役割 (2008)
  • Author(s): 松本真典
  • Journal Title: 臨床免疫・アレルギー科 50 Pages: 437-442
• [Journal Article] CD4^+ CD25^+ regulatory T cells in the small intestinal lamina propria show an effector/memory phenotype (2008)
  • Author(s): Guo, Z.
  • Journal Title: International Immunology 20 Pages: 307-315
  • Peer Reviewed
• [Presentation] M. PSGL-1 negatively regulates T cell immune responses in vivo. (2008)
  • Author(s): Matsumoto, M
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Pripheral lymphopenia in moesin-deficient mice (2008)
  • Author(s): Serigano, N
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] PSGL-1 negatively regulates T cell immune responses in vivo (2008)
  • Author(s): Matsumaoto, M.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Peripehral lymphopenia in moesin-deficient mice (2008)
  • Author(s): Serigano, N.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] CD4^+CD25^+ regulatory T cells in the small intestinal lamina propria show an effector/memory phenotype (2007)
  • Author(s): Guo, Z.
  • Organizer: 第37回日本免疫学会総会・学術総会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-21
• [Presentation] An atni-adhesive role of PSGL-1 in T cells (2007)
  • Author(s): Matsumoto, M
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-20
• [Presentation] An endothelial L-selectin is important for E-selectin-mediated neutrophil rolling in inflammation (2007)
  • Author(s): Shigeta, A
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-20
• [Presentation] CD4+CD25+ regulatory T cells in thesmall intestinal lamina propria show aneffector/memory phenotype (2007)
  • Author(s): Guo, Z
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-20
• [Presentation] An anti-adhesive role of PSGL-1 on T cells (2007)
  • Author(s): Matsumoto, M.
  • Organizer: 第37回日本免疫学会総会・学術総会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-20
• [Presentation] An endothelial L-selectin ligand is important for E-selectin-mediated neutrophil rolling in inflammation (2007)
  • Author(s): Shigeta, A.
  • Organizer: 第37回日本免疫学会総会・学術総会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-20
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/act/act_hirata.php
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/act/act_hirata.php
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/COE/project/pro14.htm1