Construction of efficient expression system of anti-tumor marker protein-monoclonal antibodies in transgenic plant.

• Project/Area Number: 19590573
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Toho University
• Principal Investigator: SATOH Hiroyuki Toho University, 理学部, 准教授 (80187228)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 肝細胞癌 / αフェトプロテイン / PIVKA-II / 遺伝子組換え植物 / ペルオキシダーゼ / モノクローナル抗体 / 腫瘍マーカー検査 / AFP / plantibody / 腫瘍マーカー / ワサビ

Research Abstract

To reduce the production cost of monoclonal antibody for diagnosis, we tried to express gamma-globulin cDNAs of AFP and PIVKA-II in transgenic plant for the diagnosis of hepatocellular carcinoma. After we cloned the cDNAs, construction of cDNAs into scFv was made for its expression in transgenic-plant. As the other approach, we found some novel peroxidases possess higher activity from Japanese wsabi radish than that of horse radish peroxidase.

Research Products

• [Journal Article] A Proteomic Profiling Approach to Reveal a Novel Role of BnD22 (Brassica napus drought 22 kD)/Water-Soluble Chlorophyll binding Protein in young leaves during Nitrogen Remobilization induced by Stressful conditions (2008)
  • Author(s): Desclos, et al.
  • Journal Title: Plant Physiology 147 Pages: 1830-1844
  • Peer Reviewed
• [Journal Article] Structural mechanism and photoprotective function of water-soluble chlorophyll-binding protein. (2007)
  • Author(s): Horigome, et. al.
  • Journal Title: J. Biol. Chem., 282 Pages: 6525-6531
  • Peer Reviewed
• [Presentation] ニホンワサビペルオキシダーゼのcDNAクローニング (2008)
  • Author(s): 小嶋拓也, 他
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 病原性放線菌Nocardia farcinicaにおけるアミノ配糖体リン酸化酵素遺伝子の同定 (2008)
  • Author(s): 藤井匠子, 他
  • Organizer: 2008年度日本放線菌学会大会
  • Place of Presentation: 山梨
  • Year and Date: 2008-07-10
• [Presentation] ニホンワサビペルオキシダーゼのcDNAクローニング (2008)
  • Author(s): 小嶋拓也, 佐藤浩之
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸