Ultrafast determination of drugs in biological samples by column-switching LC/MS
Project/Area Number |
19590683
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Legal medicine
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Research Institution | Aichi Medical University |
Principal Investigator |
ARINOBU Tetsuya Aichi Medical University, 医学部, 准教授 (30329776)
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Project Period (FY) |
2007 – 2009
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Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 法中毒学 / 迅速分析 / 分析科学 / 有機化学 / 質量分析 / 高速液体クロマトグラフィー / 血中薬物 |
Research Abstract |
Theophylline and caffeine are natural alkaloids widely being ingested, and show various pharmacological effects in humans. Caffeine is a constituent of coffee and other beverages and included in many medicines. Theophylline is used as a bronchodilator and also formed as a metabolite of caffeine in humans ; theophylline poisoning is occasionally encountered in the field of forensic toxicology, because of its relatively narrow safe range of therapeutic doses. They have been analyzed by various methods, such as gas chromatography (GC), GC-mass spectrometry (MS), high-performance (HP) liquid chromatography (LC)-MS. In the existing analytical methods for the drugs, the sample pretreatments such as liquid-liquid extraction, solid-phase extraction, filtration or centrifugation were usually required to remove proteins and other impurities contained in biological matrices. The analytical methods including time-consuming multi-step sample pretreatments are unsuitable for rapid detection of poisonous materials in human specimens. To avoid such sample pretreatments, special HPLC columns, which enabled direct injection of crude biological samples, were developed ; our group applied them to compounds of forensic toxicological interest. Furthermore, backflush column-switching system using one of special HPLC columns have become a powerful analytical tool for simultaneous determinations of various drugs in biological samples. In this study, we have established an automated on-line method for ultra-fast determination of theophylline and caffeine in human serum by HPLC-MS with backflush column-switching and a monolithic separation column. To our knowledge, our method presented in this study is the fastest MS determination technique for theophylline and caffeine in human serum using the conventional HPLC instrument.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] High-throughput determination of theophylline and caffeine in human serum by conventional liquid chromatography-mass spectrometry.2009
Author(s)
Arinobu T, Hattori H, Kumazawa T, Lee X-P, Mizutani Y, Katase T, Kojima S, Omori T, Kaneko R, Ishii A, Seno H.
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Journal Title
Forensic Toxicol. 27
Pages: 1-6
Related Report
Peer Reviewed
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[Journal Article] High-throughput determination of theophylline and caffeine in human serum by conventional liquid chromatography-mass spectrometry2009
Author(s)
Tetsuya Arinobu, Hideki Hattori, Takeshi Kumazawa, Xiao-Pen Lee, Yoko Mizutani, Takao Katase, Sadao Kojima, Takayuki Omori, Rina Kaneko, Akira Ishii, Hiroshi Seno
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Journal Title
Forensic Toxicology 27
Pages: 1-6
Related Report
Peer Reviewed
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[Journal Article] Rapid analysis of sertraline, fluvoxamine, and paroxetine in serum specimens by LC-MS-MS using a new polymer column.2007
Author(s)
Hattori H, Ito K, Iwai M, Arinobu T, Mizutani Y, Kumazawa T, Ishii A, Suzuki O, Seno H.
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Journal Title
Forensic Toxicol. 25(2)
Pages: 100-103
Related Report
Peer Reviewed
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[Journal Article] Rapid analysis of sertraline, fluvoxamine, and paroxetine in serum specimens by LC-MS-MS using a new polymer column2007
Author(s)
Hideki Hattori, Kenjiro Ito, Masae Iwai, Tetsuya Arinobu, Yoko Mizutani, Takeshi Kumazawa, Akira Ishii, Osamu Suzuki, Hiroshi Seno
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Journal Title
Forensic Toxicology 25
Pages: 100-103
Related Report
Peer Reviewed
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[Presentation] Determination of phenothiazines in human serum by HPLC/SSI-MS using backflush column-switching.2007
Author(s)
Arinobu T, Aoki R, Kumazawa T, Lee X-P., Ishii A, Kaneko R, Kojima S, Noguchi H, Suzuki O, Hattori H, Seno H
Organizer
12^<th> International meeting on recent developments in pharmaceutical analysis
Place of Presentation
Elba, Italy
Related Report
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[Presentation] DETERMINATION OF PHENOTHIAZINES IN HUMAN SERUM BY HPLC/SSI-MSUSING BACKFLUSH COLUMN-SWITCHING2007
Author(s)
T. Arinobu, R. Aoki, T. Kumazawa, X-P. Lee, A. Ishii, R. Kaneko, S. Kojima, H. Noguchi, O. Suzuki, H. Hattori, H. Seno
Organizer
12th International meeting on recent developments in pharmaceutical analysis
Place of Presentation
Elba, Italy
Related Report