|Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
The cytokines investigated were IFN-γ, IL-6, IL-10, IL-12p70, MCP-1 and TNF. First, we investigated the effect of paraquat (Pq) on cytokine levels using the model of Pq-poisoned mice. As the result, IL-6 and MCP-1 levels, both mRNA in tissues and protein levels in blood, significantly increased within 5 days after Pq exposure. The former may be an early marker and the latter be a late marker for lung acute damage. Now we examine the effect of anti IL-6 antibody to Pq-poisoned damage, which need further investigation. Next, we examined the effects of methamphetamine (MA) on cytokine levels using a water-immersion restraint stress (WRS) mouse model. The levels of the serum cytokines IL-6, IL-10 and TNF were increased by stress, and were markedly increased by MA under stress conditions ; in particular, the level of IL-6 was synergistically increased. On the other hand, MA resulted in significant suppression of the stress-induced gastric injury, although further studies are necessary to resolve the mechanism. We investigated the involvement of the GR polymorphism in stress response using ICR mice. Our results suggest that the GR gene polymorphism has a significant impact on the stress-induced output, including cytokines, corticosterone, Hsp70 levels in serum, and the gastric lesion index. These studies provide insights into the role of GR in individual responses to stress.