Intercellular junctional complexes replaced with slit diaphragms

• Project/Area Number: 19590940
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: YAOITA Eishin Niigata University, 医歯学系, 准教授 (00157950)
• Co-Investigator(Kenkyū-buntansha): YAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737) ; YOSHIDA Yutaka 新潟大学, 医歯学系, 講師 (40182795)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 腎臓 / 足細胞 / 細胞間結合 / 密着結合 / スリット膜 / タイト結合 / クローディン / 賢臓

Research Abstract

腎臓は細胞外液の質量を一定に保ち、生命維持に必須な働きをしている.そのために常に一定量の糸球体濾過を維持しなければならない.糸球体濾過ための重要な構成要素になっているのがスリット膜である.スリット膜は、糸球体を覆っている上皮細胞(足細胞)の細胞間を繋ぐ結合装置であり、細胞傷害時に他の細胞間結合装置と移行し合っている.スリット膜の分子構造は明らかになりつつあるが、これと移行し合い、糸球体ろ過調節に与っている他の細胞間結合装置については、ほとんど明らかにされていない.本研究では、この細胞間結合装置の構成分子としてクローディン-6 があることを明らかにした.

Research Products

• [Journal Article] Identification and localization of novel genes preferentially expressed in human kidney glomerulus (2009)
  • Author(s): Cuellar LM, Fujinaka H, Yamamoto K, Miyamoto M, Tasaki M, Zhao L, Tamer I, Yaoita E, Yoshida Y, Yamamoto T
  • Journal Title: Nephrology (Carlton) 14 Pages: 94-104
  • Peer Reviewed
• [Journal Article] Identification and localization of novel genes preferentially expressed in human kidney glomerulus. (2009)
  • Author(s): Cuellar LM
  • Journal Title: Nephrology (Carlton) 14 Pages: 94-104
  • Peer Reviewed
• [Journal Article] Overview of kidney and urine proteome databases (2008)
  • Author(s): Yoshida Y, Miyamoto M, Bo X, Yaoita E, Yamamoto T
  • Journal Title: Contrib Nephrol 160 Pages: 186-97
  • Peer Reviewed
• [Journal Article] Claudin-6 localized in tight junctions of rat podocytes (2008)
  • Author(s): Zhao L, Yaoita E, Nameta M, Zhang Y, Cuellar LM, Fujinaka H, Xu B, Yoshida Y, Hatakeyama K, Yamamoto T
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 294(6) Pages: 1856-62
  • Peer Reviewed
• [Journal Article] Claudin-6 localized in tight junctions of rat podocytes. (2008)
  • Author(s): Zhao L
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. 294
  • Peer Reviewed
• [Journal Article] Overview of kidney and urine proteome databases. (2008)
  • Author(s): Yoshida Y
  • Journal Title: Contrib Nephrol. 2008 ; 160:186-97 160 Pages: 186-197
  • Peer Reviewed
• [Journal Article] Claudin-6 localized in tight junctions of rat podocytes. (2008)
  • Author(s): Zhao L
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. (印刷中)
  • Peer Reviewed
• [Journal Article] In-depth proteomic profiling of the normal human kidney glomerulus using two-dimensional protein prefractionation in combination with liquid chromatography-tandem mass spectrometry (2007)
  • Author(s): Miyamoto M, Yoshida Y, Taguchi I, Nagasaka Y, Tasaki M, Zhang Y, Xu B, Nameta M, Sezaki H, Cuellar LM, Osawa T, Morishita H, Sekiyama S, Yaoita E, Kimura K, Yamamoto T
  • Journal Title: J Proteome Res 6(9) Pages: 3680-90
  • Peer Reviewed
• [Journal Article] Podocin participates in the assembly of tight junctions between foot processes in nephrotic podocytes (2007)
  • Author(s): Shono A, Tsukaguchi H, Yaoita E, Nameta M, Kurihara H, Qin XS, Yamamoto T, Doi T
  • Journal Title: J Am Soc Nephrol 18(9) Pages: 2525-33
  • Peer Reviewed
• [Journal Article] Anti-perforin antibody treatment ameliorates experimental crescentic glomerulonephritis in WKY rats (2007)
  • Author(s): Fujinaka H, Yamamoto T, Feng L, Nameta M, Garcia G, Chen S, El-shemi AA, Ohshiro K, Katsuyama K, Yoshida Y, Yaoita E, Wilson CB
  • Journal Title: Kidney Int 72(2) Pages: 823-30
  • Peer Reviewed
• [Journal Article] Periglomerular accumulation of dendritic cells in rat crescentic glomerulonephritis (2007)
  • Author(s): Fujinaka H, Nameta M, Kovalenko P, Matsuki A, Kato N, Nishimoto G, Yoshida Y, Yaoita E, Naito M, Kihara I, Tomizawa S, Yamamoto T
  • Journal Title: J Nephrol 20(3) Pages: 357-63
  • Peer Reviewed
• [Journal Article] Rho-family small GTPases are involved in forskolin-induced cell-cell contact formation of renal glomerular podocytes in vitro (2007)
  • Author(s): Gao SY, Li CY, Shimokawa T, Terashita T, Matsuda S, Yaoita E, Kobayashi N
  • Journal Title: Cell Tissue Res 328(2) Pages: 391-400
• [Journal Article] Podocin participates in the assembly of tight junctions between foot processes in nephrotic podocytes. (2007)
  • Author(s): Shono A
  • Journal Title: J Am Soc Nephrol. 18 Pages: 2525-2533
  • Peer Reviewed
• [Journal Article] In-depth proteomic profiling of the normal human kidney glomerulus using two-dimensional protein prefractionation in combination with liquid chromatography-tandem mass spectrometry. (2007)
  • Author(s): Miyamoto M
  • Journal Title: J Proteome Res. 6 Pages: 3680-3690
  • Peer Reviewed
• [Presentation] Top-down proteomic analysis and identification of tyrosine-phosphorylated proteins in the glomerulus of normal rat kidney (2008)
  • Author(s): 張榮
  • Organizer: 第51回日本腎臓学会学術総会
  • Place of Presentation: 福岡
• [Presentation] Tyrosine-phosphorylated proteins are predominantly localized in the glomerulus of normal rat kidney (2008)
  • Author(s): Zhang Y
  • Organizer: 第7回HUPO国際学会
  • Place of Presentation: アムステルダム
• [Presentation] Proteomic approach of glomerulus microdissected from biopsy samples of human kidney (2008)
  • Author(s): Koda R
  • Organizer: 第7回HUPO国際学会
  • Place of Presentation: アムステルダム
• [Presentation] Claudin-6 is a component of tight junctions of rat podocytes. (2007)
  • Author(s): Zhao L
  • Organizer: アメリカ腎臓学会学術総会
  • Place of Presentation: アメリカ合衆国サンフランシスコ
  • Year and Date: 2007-11-02
• [Presentation] Expression of the chemokine ractalkine in podocytes in normal and roteinuric glomeruli (2007)
  • Author(s): Fujinaka H
  • Organizer: 第40回アメリカ腎臓学会学術総会
  • Place of Presentation: サンフランシスコ
• [Presentation] Claudin-6 is a component of tight junctions of rat podocytes (2007)
  • Author(s): Zhao L
  • Organizer: 第40回アメリカ腎臓学会学術総会
  • Place of Presentation: サンフランシスコ
• [Presentation] In-depth profiling of the normal human kidney glomerulus proteome by Two-dimensional protein prefractionation coupled with nanoflow LC-MS/MS (2007)
  • Author(s): Miyamoto M
  • Organizer: 第40回アメリカ腎臓学会学術総会
  • Place of Presentation: サンフランシスコ
• [Presentation] Handling and optimization of urinary protein preparation for 2-DE based Proteomic analysis and 2D DIGE quantitative analysis of urine proteomes of healthy Male children and adults (2007)
  • Author(s): Yoshida Y
  • Organizer: 第6回HUPO国際学会
  • Place of Presentation: ソウル
• [Presentation] In-depth profiling of the normal human kidney glomerulus proteome by two-Dimensional protein prefractionation coupled with nanoflow LC-MS/MS (2007)
  • Author(s): Yoshida Y
  • Organizer: 第6回HUPO国際学会
  • Place of Presentation: ソウル
• [Presentation] ラット足細胞におけるクローディン-6 (2007)
  • Author(s): 矢尾板永信
  • Organizer: 第50回日本腎臓学会学術総会
  • Place of Presentation: 松浜