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Comprehensive analysis of prostasin activators and inhibitors and its application for the elucidation of blood pressure regulation

Research Project

Project/Area Number 19590956
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionKumamoto University

Principal Investigator

KITAMURA Kenichiro  Kumamoto University, 医学部・附属病院, 講師 (10304990)

Co-Investigator(Kenkyū-buntansha) 冨田 公夫  熊本大学, 大学院・医学薬学研究部, 教授 (40114772)
Co-Investigator(Renkei-kenkyūsha) TOMITA Kimio  熊本大学, 大学院・医学薬学研究部, 教授 (40114772)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords水 / 電解質代謝学 / ナトリウムチャネル / セリンプロテアーゼ / プロスタシン / 高血圧
Research Abstract

私たちは上皮型Naチャネル活性化因子であるセリンプロテアーゼのプロスタシンに対するアクチベーターを同定し、プロテアーゼによる血圧調節機構の分子基盤を解明しようと考えた。ラット腎臓組織からzymography法を用いて、プロスタシンのアクチベーター活性を指標に30kDaのタンパク質の分離に成功した。LC-MS/MS法によりカリクレインであることが判明し、現在、カリクレイン-プロスタシンカスケードが上皮型Naチャネル活性に与える影響を検討中である。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (19 results)

All 2009 2008 2007

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (2 results) Book (2 results)

  • [Journal Article] Aberrant ENaC activation in Dahl salt-sensitive rats2009

    • Author(s)
      Kakizoe, Y., Kitamura, K., Wakida, N., Ko, T., Maekawa, A., Miyoshi, T., Shiraishi, N., Adachi, M., Zhang, Z., Masilamani, S. and Kimio Tomita
    • Journal Title

      J. Hypertens (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Urinary prostasin in humans : relationships among prostasin, aldosterone and epithelial sodium channel activity2009

    • Author(s)
      Koda, A., Wakida, N., Toriyama, K., Yamamoto, K., Iijima, H., Tomita, K., and Kitamura, K.
    • Journal Title

      Hypertens. Res. 32

      Pages: 276-281

    • NAID

      10024830568

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Camostat mesilate inhibits prostasin activity, and reduces blood pressure and renal injury in salt-sensitive hypertension2009

    • Author(s)
      Maekawa, A., Kakizoe, Y., Miyoshi, T., Wakida, N., Ko, T., Shiraishi, N., Adachi, M., Tomita, K., and Kitamura, K.
    • Journal Title

      J. Hypertens 27

      Pages: 181-189

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of intravenous iron administration frequency on oxidative stress in chronic hemodialysis patients2008

    • Author(s)
      Kitamura, K., Anraku, M., Shintomo, R., Takeuchi, K., Ikeda, H., Nagano, J., Ko, T., Mera, K., Tomita, K., and Otagiri, M.
    • Journal Title

      Clin. Biochem. 41

      Pages: 1168-1174

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma urate level is directly regulated by a voltage-driven urate efflux transporter URATv1 (SLC2A9) in humans2008

    • Author(s)
      Anzai, N., Ichida, K., Jutabha, P., Kimura, T., Babu, E., Jin, C.J., Srivastava, S., Kitamura, K., Hisatome, I., Endou, H., and Sakurai, H.
    • Journal Title

      J. Biol. Chem 283

      Pages: 26834-26838

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] CD36 is one of important receptors promoting renal tubular injury by advanced oxidation protein products2008

    • Author(s)
      Iwao, Y., Nakajou, K., Nagai, R., Kitamura, K., Anraku, M., Maruyama, T., and Otagiri, M.
    • Journal Title

      Am. J. Physiol(Renal Physiol.) 295

    • NAID

      120005952926

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term plasma levels and dose modulation of alacepril in patients with chronic renal failure2008

    • Author(s)
      Nonoguchi, H., Kiyama, S., Kitamura, K., Naruse, M., Tomita, M., Tazoe, N., Tajiri, M., Nakayama, Y., Kohda, Y., Inoue, T., and Tomita, K.
    • Journal Title

      Hypertens Res. 31

      Pages: 29-36

    • NAID

      10021260933

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma urate level is directly regulated by a voltage-driven urate efflux transporter URATvl (SLC2A9) in humans2008

    • Author(s)
      Anzai, N., et al.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 26834-26838

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CD36 is one of important receptors promoting renal tubular injury by advanced oxidation protein products2008

    • Author(s)
      Iwao, Y., et al.
    • Journal Title

      Am. J. Physiol. (Renal Physiol. ) 295

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of intravenous iron administration frequency on oxidative stress in chronic hemodialysis patients2008

    • Author(s)
      Anraku, M., et al.
    • Journal Title

      Clin. Biochem. 41

      Pages: 1168-1174

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term plasma levels and dose modulation of alacepril in patients with chronic renal failure2008

    • Author(s)
      Nonoguchi, H., et. al.
    • Journal Title

      Hypertens. Res. 31

      Pages: 26-39

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An oral adsorbent, AST-120 protects against the progression of oxidative stress by reducing the accumulation of indoxyl sulfate in the systemic circulation in renal failure2007

    • Author(s)
      Shimoishi, K., Anraku, M., Kitamura, K., Tasaki, Y., Taguchi, K., Hashimoto, M., Fukunaga, E., Maruyama, T., and Otagiri, M.
    • Journal Title

      Pharm. Res 24

      Pages: 1283-1289

    • NAID

      120005894409

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Olmesartan on Oxidative Stress in Hemodialysis Patients2007

    • Author(s)
      Kadowaki, D., Anraku, M., Tasaki, Y., Kitamura, K., Wakamatsu, S., Tomita, K., Gebicki, J. M., Maruyama, T., and Otagiri, M.
    • Journal Title

      Hypertens. Res. 30

      Pages: 395-402

    • NAID

      10019537151

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] An oral adsorbent,AST-120 protects against the progression of oxidative stress by reducing the accumulation of indoxyl sulfate in the systemic circulation in renal failure2007

    • Author(s)
      Shimoishi, K., et. al.
    • Journal Title

      Pharm. Res. 24

      Pages: 1283-1289

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Olmesartan on Oxidative Stress in Hemodialysis Patients2007

    • Author(s)
      Kadowaki, D., et. al.
    • Journal Title

      Hypertens. Res. 30

      Pages: 395-402

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Camostat mesilate attenuates hypertension and kidney injury in Dahl salt-sensitive rats2008

    • Author(s)
      Kakizoe, Y., et.al.
    • Organizer
      American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2008-11-07
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] プロテアーゼとプロテアーゼインヒビターの相互作用による生体Na代謝制御の分子基盤2007

    • Author(s)
      北村健一郎
    • Organizer
      日本腎臓学会学術総会
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2007-05-25
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Book] 医学のあゆみ2007

    • Author(s)
      北村健一郎
    • Total Pages
      4
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2008 Final Research Report
  • [Book] 医学のあゆみ2007

    • Author(s)
      北村 健一郎
    • Total Pages
      4
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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