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Biological analysis of phosphorylated α-synuclein as a therapeutic target for Parkinson's disease

Research Project

Project/Area Number 19590979
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionYamagata University

Principal Investigator

ARAWAKA Shigeki  Yamagata University, 大学院・医学系研究科, 助教 (00344789)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords神経生化学 / 神経病態科学 / 神経変性疾患 / パーキンソン病 / αシヌクレイン / リン酸化 / キナーゼ / 脳神経疾患 / 神経科学
Research Abstract

パーキンソン病(PD)のレビー小体を構成するαシヌクレイン(αS)の129番目セリン残基のリン酸化が、αSの毒性を促進させる可能性がある。本研究課題はαS リン酸化反応を検討し、1)SH-SY5Y細胞でGRK3、GRK5、GRK6、CK2 がリン酸化に関与、2)αSの細胞内局在により寄与するキナーゼが異なる、3)AAVウイルスでαSとキナーゼをラット中脳黒質に共接種しドパミン神経細胞死を生ずる系の作製に成功した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (10 results)

All 2009 2008 2007

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (7 results)

  • [Journal Article] Contribution of endogenous G-coupled receptor kinases to Ser129 phosphorylation of -synuclein in HEK293 cells2009

    • Author(s)
      Sakamoto M, Arawaka S (corresponding author), Hara S, Sato H, Cui C, Machiya Y, Koyama S, Wada M, Kawanami T, Kurita K, Kato T
    • Journal Title

      Biochem Biophys Res Commun 384

      Pages: 378-382

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] N-terminal region of alpha-synuclein is essential for the fatty acid-induced oligomerization of the molecules2008

    • Author(s)
      Karube H, Sakamoto M, Arawaka S (corresponding author), Hara S, Sato H, Ren CH, Goto S, Koyama S, Wada M, Kawanami T, Kurita K, Kato T
    • Journal Title

      FEBS Lett 582

      Pages: 3693-3700

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] N-terminal region of alpha-synuclein is essential for the fatty acid-induced oligomerization of the molecules2008

    • Author(s)
      Karube H
    • Journal Title

      FEBS letters 582

      Pages: 3693-3700

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 内因性GRKsおよびCK1εのαシヌクレインSer129リン酸化反応への寄与2008

    • Author(s)
      原進、荒若繁樹
    • Organizer
      日本生化学学会・日本分子生物学会合同学会BMB2008
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 内因性GRKsのαシヌクレインSer129リン酸化反応への寄与2008

    • Author(s)
      原進、荒若繁樹
    • Organizer
      第49回日本神経学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Biological contribution of G-protein-coupled receptor kinases to phosphorylation of alpha-synuclein at S129 in the pathogenesis of sporadic Parkinson's disease2007

    • Author(s)
      Hara S、Arawaka S
    • Organizer
      Society of Neuroscience 2007
    • Place of Presentation
      サンディエゴ、アメリカ合衆
    • Year and Date
      2007-11-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Biological contribution of G-protein-coupled receptor kinases to phosphorylation of alpha-synuclein at Ser129 in pathogenesis of sporadic Parkinson's disease2007

    • Author(s)
      原 進
    • Organizer
      Society of Neuroscience 2007
    • Place of Presentation
      アメリカ合衆国
    • Year and Date
      2007-11-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] Contribution of endogenous GRKs to phosphorylation of alpha-synuclein at S129 in cultured cells2007

    • Author(s)
      Sato H 、Arawaka S
    • Organizer
      International psychogeriatric association
    • Place of Presentation
      大阪
    • Year and Date
      2007-10-17
    • Related Report
      2008 Final Research Report
  • [Presentation] Contribution of endogenous GRKs to phosphorylation of alpha-synuclein at Ser129 in cultured cells2007

    • Author(s)
      佐藤 裕康
    • Organizer
      International psychogeriatric association 2007
    • Place of Presentation
      大阪
    • Year and Date
      2007-10-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] GRK5によるα-synucleinセリン129リン酸化に関する細胞生物学的検討2007

    • Author(s)
      荒若 繁樹
    • Organizer
      第48回日本神経学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2007-05-17
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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