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Novel neuroprotective factors against both ALS and Alzheimer's disease and the action mechanism study

Research Project

Project/Area Number 19591007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionKeio University

Principal Investigator

KITA Yoshiko  Keio University, 医学部, 講師 (00327496)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsADNF / HN / ALS / アルツハイマー病 / コリベリン / ヒューマニン(HN) / 神経変性疾患 / MND / G93A-SOD1
Research Abstract

抗ALS及び抗アルツハイマー活性を有する神経保護因子コリベリン(ADNF-HN誘導体)の高活性(fM)のメカニズム解明をめざして、構成成分であるADNF、AGAC8R-HNG17と共にキャラクタライゼーションを実施した。CD、分析用超遠心によるキャラクタライゼーションの結果は今までの2量体化説に反し、コリベリン、ADNF、AGAC8R-HNG17とも単量体である事が、CD及び、分析用超遠心の測定から強く示唆された。またターゲット分子の1つとしてFPRL-1が考えられる。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (34 results)

All 2009 2008 2007 Other

All Journal Article (22 results) (of which Peer Reviewed: 21 results) Presentation (10 results) Remarks (2 results)

  • [Journal Article] Active form of Neuroprotective Humanin, HN and inactive analog, S7A-HN, are monomeric and disordered in aqueous phosphatesolution at pH6.0 ; No correlation of solution structure with activity2009

    • Author(s)
      Arisaka F, Arakawa T, Niikura T, Kita Y
    • Journal Title

      Protein Pept Lett 16

      Pages: 132-137

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synergistic virus inactivation effects of arginine2009

    • Author(s)
      Arakawa T, Kita Y, Koyama H
    • Journal Title

      Biotechnology J 4

      Pages: 174-178

    • Related Report
      2008 Annual Research Report 2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] MEP chromatography of antibody and Fc-fusion protein using aqueous arginine solution2009

    • Author(s)
      Arakawa T, Kita Y, Sato H, Ejima D
    • Journal Title

      Protein Expt. Purf 63

      Pages: 158-163

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Active form of Neuroprotective Humanin, HN, and inactive alalog, S7A-HN, are monomeric and disordered in aqueous phosphate solution at pH6.0 ; No correlation of solution structure with activity2009

    • Author(s)
      Arisaka F, Arakawa T, Niikura T, Kita Y
    • Journal Title

      Protein Pept. Lett. 16

      Pages: 132-137

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MEP chromatography of antibody and Fc-fusion protein usingaqueous arginie solution2009

    • Author(s)
      Arakawa T, Kita Y, Sato H, Ejima D
    • Journal Title

      Protein Expr. Purf. 63

      Pages: 158-163

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A rescue factor for Alzheimer's disease : discovery, activity, structure and mechanism2008

    • Author(s)
      Arakawa T, Kita Y, Niikura T
    • Journal Title

      Curr. Med. Chem 15

      Pages: 2088-2098

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The structure analysis of Humanin analog, AGAC8R-HNG17, by circular dichroism and sedimentation equilibrium : Comparison with the parent molecule2008

    • Author(s)
      Arisaka F, Niikura T, Arakawa T, Kita Y
    • Journal Title

      Int. J. Biol. Macromol 43

      Pages: 88-93

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The complex structure transition of Humanin peptides by sodium dodecylsulfate and trifluoroethanol2008

    • Author(s)
      Kita Y, Niikura T, Arisaka F, Arakawa T
    • Journal Title

      Protein Pept. Lett 15

      Pages: 510-515

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Solvent modulation of column chromatography2008

    • Author(s)
      Arakawa T, Kita Y, Ejima D, Gagnon P
    • Journal Title

      Protein Pept. Lett 15

      Pages: 544-555

    • Related Report
      2008 Annual Research Report 2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as anegative regulator for N-terminal palmitoylation of Sonic hedgehog2008

    • Author(s)
      Abe Y, Kita Y, Niikura T
    • Journal Title

      FEBS J 275

      Pages: 318-331

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Solubility enhancement of gluten and organic compounds by arginine2008

    • Author(s)
      Arakawa T, Kita Y, Koyama AH
    • Journal Title

      Int. J. Pharm 355

      Pages: 220-223

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A rescue factor for Alzheimer's disease : discovery, activity, structure, and mechanism2008

    • Author(s)
      Arakawa T, Kita Y, Niikura T
    • Journal Title

      Curr. Med. Chem. 15

      Pages: 2086-2098

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The sturucture analysis of Humanin analog, AGAC8R-HNG17, by circular dichroism and sedimentation equilibrium : Comparison with the parent molecule2008

    • Author(s)
      Arisaka F, Niikura T, Arakawa T, Kita Y
    • Journal Title

      Int. J. Biol. Macromol. 43

      Pages: 88-93

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The complex structure transition of Humanin peptides by sodium dodecylsulfate and trifluoroethanol2008

    • Author(s)
      Kita Y, Niikura T, Arisaka F, Arakawa T
    • Journal Title

      Protein Pept. Lett. 15

      Pages: 510-515

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mammalian Gup l,ahomolog of Saccharomyces cerevisiae glyceroluptake/transporter 1,acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog2008

    • Author(s)
      Abe Y, Kita Y, Niikura T
    • Journal Title

      FEBS J. 275

      Pages: 318-331

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sollubility enhancement of gluten and organic compounds by arginine2008

    • Author(s)
      Arakawa T, Kita Y, Koyama AH
    • Journal Title

      Int J Pharm 355

      Pages: 220-223

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure analysis of Activity-Dependent Neurotrophic factor 9 by circular dichroism and sedimentation equilibrium2007

    • Author(s)
      Arakawa T, Niikura T, Tajima H, Arisaka F, Kita Y
    • Journal Title

      J. Mol. Neurosci 33

      Pages: 262-267

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activity dependentneurotrophic factor ADNF, determined the structure characteristics of Colvelin a fusion protein of ADNF9 and Humanin2007

    • Author(s)
      Arakawa T, Niikura T, Arisaka F, Kita Y
    • Journal Title

      J. Pept Sci 14

      Pages: 631-636

    • Related Report
      2008 Final Research Report
  • [Journal Article] Protein precipitation and denaturation by dimethyl sulfoxide2007

    • Author(s)
      Arakawa T, Kita Y, Timasheff S
    • Journal Title

      Biophys Chem 131

      Pages: 62-70

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure analysis of Activity-Dependent Neurotrophic Factor 9 by circular dichroism and sedimentation equilibium2007

    • Author(s)
      Arakawa T, Niikura T, Tajima H, Arisaka F, Kita Y
    • Journal Title

      J.Mol Neurosci 33

      Pages: 262-267

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activity-dependent neurotrophic gactor ADNF, determines the sturucturecharacteristics of Colivelin a fusion protein of ADNF9 and Humaninanalog2007

    • Author(s)
      Arakawa T, Niikura T, Arisaka F, Kita Y
    • Journal Title

      J Pept Sci 14

      Pages: 631-636

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein Precipitation and denaturation by dimethyl sulfoxide2007

    • Author(s)
      Arakawa T, Kita Y, Timasheff S
    • Journal Title

      Biophys Chem 131

      Pages: 62-70

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 結晶構造を基盤としたアルギニン塩酸塩の存在による蛋白質の水和変化の解析(Structure-based analysis reveals hydration changes induced by arginine hydrochloride)2008

    • Author(s)
      中木戸誠、田中良和、三堀麻理子、工藤基徳、江島大輔、喜多淑子、荒川力、津本浩平
    • Organizer
      第31回日本分子生物学会年会、合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Final Research Report
  • [Presentation] 結晶構造を基盤としたアルギニン塩酸塩の存在による蛋白質の水和変化の解析(structure-based analysis revealshydration changes induced by arginine hydrochloride)2008

    • Author(s)
      中木戸誠, 田中良和, 三堀麻理子, 工藤基徳, 江島大輔, 喜多淑子, 荒川力, 津本浩平
    • Organizer
      第31回日本分子生物学会年会、第81回日本生物化学会大会、合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] A Structure-Activity relationship of AGAC8R-HNG17, a component of femtomolar acting factor Colivelinagainst Alzheimer's disease insults : Comparison studied of ADNF Humanin(HN)and the analogues2008

    • Author(s)
      Kita Y, Niikura T, Arakawa T Arisaka F
    • Organizer
      SFN(Society for Neuroscience)
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2008-11-19
    • Related Report
      2008 Final Research Report
  • [Presentation] A Structure-Activity relationship of AGAC8R-HNG17, a COmponent of femtomolar acting factor Colivelin aeainst Alzheimer's. disease insults : Comparison studies of ADNF, HumaniD(HN)and the anlogues2008

    • Author(s)
      Kita Y. Niikura T, Arakawa T, Arisaka F
    • Organizer
      Neuroscinece 2008, SFN (Society for Neuroscience)
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2008-11-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Drug candidate for Alzheimer's disease and ALS : Characterization of femto molar acting neuroprotective peptides ADNF and Colivelin, a linking peptide of ADNF and HN defivative2008

    • Author(s)
      Kita Y, Niikura T, Arisaka F, Arakawa T
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Keystone, Colorado, USA
    • Year and Date
      2008-03-26
    • Related Report
      2008 Final Research Report
  • [Presentation] Drug candidate for Alzheimer's disease and ALS:Characterization of femto molar acting neuroprotective peptides ADNF and Colivelin, a linking of ADNF and HN dericative2008

    • Author(s)
      Kita Y, Niikura T, Arisaka F, Arakawa T
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Keystone,Colorado USA
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Drug candidate for AD and ALS : structure analysis of femto-molar acting neuroprotective peptides ADNF and Colivelin2007

    • Author(s)
      Kita Y, Niikura T, Tajima H, Arisaka F,Arakawa T
    • Organizer
      Neuroscinece 2007, SFN(Society of Neuroscience)
    • Place of Presentation
      San Diego, California USA
    • Year and Date
      2007-11-06
    • Related Report
      2008 Final Research Report
  • [Presentation] Drug candidate for AD and ALS:structure analysis of femto-molar acting neuroprotective peptides ADNF and Colivelin2007

    • Author(s)
      Kita Y, Niikura T, Tajima H, Arisaka F, Arakawa T
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      San Diego,California USA
    • Year and Date
      2007-11-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of femto-molar acting neuroprotective peptides, ADNF and Colivelin : Potential mechanism of their action2007

    • Author(s)
      Kita Y
    • Organizer
      Summer Neuropeptide Conference
    • Place of Presentation
      Essex Vermont, USA
    • Year and Date
      2007-09-09
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of femto-molar acting neuroportective peptides,ADNF and Colivehn:Potential mechanism of their action2007

    • Author(s)
      Kita Y
    • Organizer
      Summer Neuropeptide Conference
    • Place of Presentation
      Essex,Vermont,USA
    • Year and Date
      2007-09-09
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://web.sc.itc.keio.ac.jp/pharm/achievement/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://web.sc.itc.keio.ac.jp/pharm/achievement/index.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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