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Functional analysis of theENDOGL1 gene as a candidate disease susceptibility gene for T2D by rentivirus vector

Research Project

Project/Area Number 19591080
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionThe University of Tokushima

Principal Investigator

ITAKURA Mitsuo  The University of Tokushima, 疾患ゲノム研究センター, 教授 (60134227)

Co-Investigator(Kenkyū-buntansha) INOUE Hiroshi  徳島大学, 疾患ゲノム研究センター, 准教授 (20294639)
MORITANI Maki  徳島大学, 疾患ゲノム研究センター, 非常勤講師 (50301312)
国香 清  徳島大学, ゲノム機能研究センター, 特任助教 (30396254)
棚橋 俊仁  徳島大学, ゲノム機能研究センター, 産学官連携研究員(特任講師) (30380067)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords2型糖尿病 / 疾患感受性候補遺伝子 / ENDOGL1遺伝子 / ミトコンドリア / 遣伝子発現量 / ポリクローナル抗体 / アポトーシス / ERストレス / 疾患感受性遺伝子 / ENDOG遺伝子 / 遺伝子発現量 / 遺伝子導入法 / 細胞内局在
Research Abstract

多遺伝子性疾患の疾患感受性座位を探索する遺伝統計学手法として、患者/健常者を対象とした関連解析、および連鎖不平衡解析が多く用いられる。我々は、異なる人種で疾患感受性が報告されている候補座位に対し『遺伝子領域に配置した等間隔・高アレル頻度SNPsをマーカーとして用いる2段階絞り込み関連解析法』を独自に開発し、日本人の2型糖尿病(T2D)の疾患感受性遺伝子を探索し、遺伝統計学的に3番染色体上に新規疾患感受性候補遺伝子(ENDOGL1)を見出だした. 糖尿病発症に係わる機序を探索した結果、1)ENDOGL1遺伝子は膵β細胞に高発現し糖尿病の病態で発現が増加すること、2)ENDOGL1タンパクはミトコンドリアに局在すること、3)200mgの量ポリクローナル抗体精製に成功したこと、4)DNA/RNA nucleaseファミリーのEndo G遺伝子(アポトーシス刺激によりDNAの断片化を引き起こす)と約40%のホモロジーを持つ本遺伝子の発現が、アポトーシス誘導時に増加すること、さらに、5)ERストレスにより本遺伝子の発現量が増加することを見出だした。作製した抗体を用いて糖代謝、アポトーシスに及ぼす影響を継続して検討中である。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (43 results)

All 2009 2008 2007 Other

All Journal Article (29 results) (of which Peer Reviewed: 29 results) Presentation (9 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Variants in KCNQ1 are associated with susceptibility to type 2 diabetes mellitus2008

    • Author(s)
      Yasuda K, Itakura M, 他45名
    • Journal Title

      Nat. Genet 40

      Pages: 1092-1097

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Common coding variant in the TCF7L2 gene and study of the association with type 2 diabetes in Japanese subjects2008

    • Author(s)
      Kunika K, Itakura M, 他9名
    • Journal Title

      J. Hum. Genet 53

      Pages: 972-982

    • NAID

      10025584810

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transgenic expression of a utated cyclin-dependent kinase 4 (CDK4/R24C)in pancreatic beta-cells prevents progression of diabetes in db/dbmice2008

    • Author(s)
      Miyatvaki K, Inoue H, Itakura M, 他7名
    • Journal Title

      Diabetes, Res. Clin. Pract 82

      Pages: 33-41

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single nucleotide polymorphisms in genes encoding LKB1(STK11), TORC2(CRTC2) and AMPK alpha2-subunit(PRKAA2)and risk of type 2 diabetes2008

    • Author(s)
      Keshavarz P, Inoue H, Itakura M, 他3名
    • Journal Title

      Mol. Genet. Metab 9

      Pages: 200-209

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lack of association of genetic variation in chromosome region 15q14-22.1 with type 2 diabetes in a Japanese population2008

    • Author(s)
      Yamaguchi Y, Itakura M 他13名
    • Journal Title

      BMC Med. Genet 9

      Pages: 22-33

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The association of genetic variants in Kruppel-like factor 11 and Type 2 diabetes in the Japanese population2008

    • Author(s)
      Tanahashi T, Itakura M, 他10名
    • Journal Title

      Diabet. Med. 25

      Pages: 19-26

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Association of STAT4 with susceptibility to rhetuuatoid arthritis and systemic lupus erythematosus in the Japanese population2008

    • Author(s)
      Kobayashi S, Itakura M 他23名
    • Journal Title

      Arthritis. Rheum 58

      Pages: 1940-1946

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Altered cellular immunity in transgenic mice with T cell-specific expression of human D4-guanine diphosphate-dissociation inhibitor (D4-GDI)2008

    • Author(s)
      Kondoh K, Itakura M, 他6名
    • Journal Title

      Int. Immunol 20

      Pages: 1299-1311

    • NAID

      10024357212

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Variants in KCNQ1 are associated with susceptibility to type 2 diabetes mellitus.2008

    • Author(s)
      Yasuda K
    • Journal Title

      Nat. Genet. 40

      Pages: 1092-1097

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Common coding variant in the TCF7L2 gene and study of the association with type 2 diabetes in Japanese subjects.2008

    • Author(s)
      Kunika K
    • Journal Title

      J. Hum. Genet. 53

      Pages: 972-982

    • NAID

      10025584810

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgenic expression of a mutated cyclin-dependent kinase 4(CDK4/R24C) in pancreatic beta-cells prevents progression of diabetes in db/db mice.2008

    • Author(s)
      Miyawaki K
    • Journal Title

      Diabetes. Res. Clin. Pract. 82

      Pages: 33-41

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single nucleotide polymorphisms in genes encoding LKB1(STK11), T0RC2(CRTC2) and AMPK alpha2-subunit (PRKAA2) and risk of type 2 diabetes.2008

    • Author(s)
      Keshavarz P
    • Journal Title

      Mol Genet Metab 9

      Pages: 200-209

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lack of association of genetic variation in chromosome region 15ql4-22.1 with type 2 diabetes in a Japanese population.2008

    • Author(s)
      Yamaguchi Y
    • Journal Title

      BMC Med. Genet. 9

      Pages: 22-33

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The association of genetic variants in Kruppel-like factor 11 and Type 2 diabetes in the Japanese population.2008

    • Author(s)
      Tanahashi T
    • Journal Title

      Diabet. Med. 25

      Pages: 19-26

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of STAT4 with susceptibility to rheumatoid arthritis and systemic lupus erythematosus in the Japanese population.2008

    • Author(s)
      Kobayashi S
    • Journal Title

      Arthritis. Rheum. 58

      Pages: 1940-1946

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Altered cellular immunity in transgenic mice with T cell-specific expression of human D4-guanine diphosphate-dissociation inhibitor (D4-GDI).2008

    • Author(s)
      Kondoh K
    • Journal Title

      Int. Immunol. 20

      Pages: 1299-1311

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lack of association of genetic variation in chromosome region 15q14-22.1 with type 2 diabetes in a Japanese population.2008

    • Author(s)
      Yamaguchi Y.
    • Journal Title

      BMC Med.Genet (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The association of genetic variants in Kruppel-like factor 11 and Type 2 diabetes in the Japanese population2008

    • Author(s)
      Tanahashi T.
    • Journal Title

      Diabetic Med. 25

      Pages: 19-26

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single nucleotide polymorphisms in genes encoding LKB1(STK11),TORC2(CRTC2) and AMPK alnha2-subunit(PRKAA2) and risk of tune 2 diabetes.2008

    • Author(s)
      Keshavarz P.
    • Journal Title

      Mol. Genet. Metab. 9

      Pages: 200-209

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The frequency of the G/G genotype of resistin single nucleotide polymorphism at-420 appears to be increased in younger onset type 2 diabetes2007

    • Author(s)
      0chi M, Itakura M, 他14名
    • Journal Title

      Diabetes 56

      Pages: 2834-2838

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of sample size effect on the identification of haplotype blocks2007

    • Author(s)
      Osabe D, Itakura M, 他10名
    • Journal Title

      BMC Bioinformatias 14

      Pages: 200-210

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic association of single nucleotide polymorphisms in endonuclease G-like 1 gene with type 2 diabetes in a Japanese population2007

    • Author(s)
      Moritani M, Itakura M, 他16名
    • Journal Title

      Diabetologia 50

      Pages: 1218-1227

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] NPs in the KCNJ11-ABCC8 gene locus are associated with type 2 diabetes and blood pressure levels in the Japanese population2007

    • Author(s)
      Sakamoto Y, lnoue H, Itakura M, 他10名
    • Journal Title

      J. Hum. Genet 52

      Pages: 781-793

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic association between the PRKCH gene encoding protein kinase Cetaisozyme and rheumatoid arthritis in the Japanese population2007

    • Author(s)
      Takata Y, Inoue H, Itakura M, 他11名
    • Journal Title

      Arthritis Rheum 56

      Pages: 30-42

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The frequency of the G/G genotype of resistin single nucleotide polymorphism at-420 appears to be increased in younger onset type 2 diabetes.2007

    • Author(s)
      Ochi M.
    • Journal Title

      Diabetes 56

      Pages: 2834-2838

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of sample size effect on the identification of haplotype blocks.2007

    • Author(s)
      Osabe D.
    • Journal Title

      BMC Bioinformatics 14

      Pages: 200-210

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic association of single nucleotide polymorphisms in endonuclease G-like 1 gene with type 2 diabetes in a Japanese population.2007

    • Author(s)
      Moritani M.
    • Journal Title

      Diabetologia 50

      Pages: 1218-1227

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SNPs in the KCNJ 11-ABCC8 gene locus are associated with type 2 diabetes and blood pressure levels in the Japanese population.2007

    • Author(s)
      Sakamoto Y.
    • Journal Title

      J. Hum. Genet 52

      Pages: 781-793

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic association between the PRKCH gene encoding protein kinase Ceta isozyme and rheumatoid arthritis in the Japanese population.2007

    • Author(s)
      Takata Y.
    • Journal Title

      Arthritis Rheum 56

      Pages: 30-42

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 3p24.3-22.1領域における2型糖尿病疾患感受性遺伝子の探索および機能解析2008

    • Author(s)
      森谷眞紀
    • Organizer
      第62回国立病院総合医学会総会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2008-11-21
    • Related Report
      2008 Final Research Report
  • [Presentation] 3p24.3-22.1領域における2型糖尿病疾患感受性遺伝子の探索および機能解析2008

    • Author(s)
      森谷 眞紀
    • Organizer
      第62回国立病院総合医学会総会
    • Place of Presentation
      東 京
    • Year and Date
      2008-11-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] 3番染色体短腕の関連解析探索により見出した日本人2型糖尿病疾患感受性ENDOGL1遺伝子の機能解析2008

    • Author(s)
      森谷眞紀
    • Organizer
      第51回目本糖尿病学会年次学術集会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2008-05-23
    • Related Report
      2008 Final Research Report
  • [Presentation] レジスチンNP-420G/G型の頻度は若年発症の2型糖尿病ほど高い2008

    • Author(s)
      越智正昭
    • Organizer
      第51回日本糖尿病学会年次学術集会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2008-05-23
    • Related Report
      2008 Final Research Report
  • [Presentation] 3番染色体短腕の関連解析探索により見出した日本人2型糖尿病疾患感受性ENDOGL1遺伝子の機能解析2008

    • Author(s)
      森谷 眞紀
    • Organizer
      第51回日本糖尿病学会年次学術集会
    • Place of Presentation
      東 京
    • Year and Date
      2008-05-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] レジスチンSNP-420 G/G型の頻度は若年発症の2型糖尿病ほど高い2008

    • Author(s)
      越智 正昭
    • Organizer
      第51回日本糖尿病学会年次学術集会
    • Place of Presentation
      東 京
    • Year and Date
      2008-05-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] 日本人2型糖尿病疾患感受性候補遺伝子の網羅的関連解析およびENDOGL1遺伝子の機能解析2007

    • Author(s)
      山口裕加
    • Organizer
      第30回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-13
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 日本人4,392名によるTCF7L2とKIAA1598遺伝子多型の関連解析2007

    • Author(s)
      国香清
    • Organizer
      第52回日本人類遺伝学会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2007-09-13
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 糖尿病モデルマウスのQTL解析とマウス系統間のハプロタイプ解析を用いた疾患感受性遺伝子の同定2007

    • Author(s)
      森谷眞紀
    • Organizer
      第50回日本糖尿病学会
    • Place of Presentation
      仙台サンプラザ
    • Year and Date
      2007-05-24
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Book] 新臨床内科学第9版2009

    • Author(s)
      板倉光夫
    • Publisher
      医学書院
    • Related Report
      2008 Final Research Report
  • [Book] 新臨床内科学 第9版2009

    • Author(s)
      板倉 光夫
    • Publisher
      医学書院
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.genome.tokushima-u.ac.jp

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.genome.tokushima-u.ac.jp

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.genome.tokushima-u.ac.jp

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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