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Analysis of the mechanisms involved in influenza virus replication and cytopathicity

Research Project

Project/Area Number 19591166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionOsaka University

Principal Investigator

NAKAYA Takaaki  Osaka University, 微生物病研究所, 特任准教授 (80271633)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords呼吸器感染症 / インフルエンザウイルス / H5N1 / アポトーシス / SARSコロナウイルス
Research Abstract

高病原性鳥インフルエンザH5N1ウイルス(H5N1-Flu)の感染・増殖と細胞傷害性との関係については今なお不明な点が多い。本研究では、ブタ呼吸器上皮由来の初代細胞による感染実験系を確立し、H5N1-Fluの感染試験を行った。その結果、H5N1-Fluによる細胞傷害性は(カスパーゼ依存的な)アポトーシス誘導が主たるカスケードであることを見出した。さらに、ヒト呼吸器由来の初代細胞でも上記と同様な結果が確認され、同システムがヒトにおけるH5N1ウイルス(呼吸器)感染の病原性を反映する可能性が示唆された。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (35 results)

All 2009 2008 2007 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (13 results) Remarks (1 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput sequencing approach2009

    • Author(s)
      Nakamura S, Yang C-S, Sakon N, Ueda M, Tougan T, Yamashita A, Goto N, Takahashi K, Yasunaga T, Ikuta K, Mizutani T, Okamoto Y, Tagami M, Morita R, Maeda N, Kawai J, Hayashizaki Y, Nagai Y, Horii T, Iida T, Nakaya T
    • Journal Title

      PLoS ONE 4(1) : e4219. Epub

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of circulating Asian H5N1 viruses by a newly established monoclonal antibody2009

    • Author(s)
      Du A, Daidoji T, Koma T, Ibrahim M-S, Nakamura S, de Silva U-C, Ueda M, Yang C-S, Yasunaga T, Ikuta K, Nakaya T
    • Journal Title

      Biochem Biophys Res Commun 378(2)Epub 2008 Nov 14. [Epub ahead of print]

      Pages: 197-202

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput sequencing approach.2009

    • Author(s)
      Nakamura S, Yang C-S, Sakon N, Ueda M, Tougan T, Yamashita A, Goto N, Takahashi K, Yasunaga T, Ikuta K, Mizutani T, Okamoto Y, Tagami M, Morita R, Maeda N, Kawai J, Hayashizaki Y, Nagai Y, Horii T, Iida T, Nakaya T.
    • Journal Title

      PLoS ONE 4(1)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HYPERLINK"http : //www. ncbi. nlm. nih. gov/pubmed/19010309? ordinalpos=1&itool=EntrezSystem2. PEntrez. Pubmed. Pubmed_ResultsPanel. Pubmed_DefaultReportPanel. Pubmed_RVDocSum"2009

    • Author(s)
      Du A, Daidoji T, Koma T, Ibrahim M-S, Nakamura S, de Silva U-C, Ueda M, Yang C-S, Yasunaga T, ^*Ikuta K, Nakaya T
    • Journal Title

      Biochem Biophys Res Commun. 378(2)

      Pages: 197-202

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells2008

    • Author(s)
      Daidoji T, Koma T, Du A, Yang C-S, Ueda M, Ikuta K, Nakaya T
    • Journal Title

      J Virol 82(22)

      Pages: 11294-307

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metagenomic diagnosis of bacterial infections2008

    • Author(s)
      Nakamura S, Maeda N, Miron I-M, Yoh M, Izutsu K, Kataoka C, Honda T, Yasunaga T, Nakaya T, Kawai J, Hayashizaki Y, Horii T, Iida T
    • Journal Title

      Emerg Infect Dis 14(11)

      Pages: 1784-86

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus2008

    • Author(s)
      Ueda M, Yamate M, Du A, Daidoji T, Okuno Y, Ikuta K, Nakaya T
    • Journal Title

      Virus Res 136(1-2)

      Pages: 91-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced anti-influenza A virus activity of (-)-epigallocatechin-3-O-gallate fatty acid monoester derivatives : Effect of alkyl chain length2008

    • Author(s)
      Mori S, Miyake S, Kobe T, Nakaya T, Fuller S-D, Kato N and Kaihatsu K
    • Journal Title

      Bioorg Med Chem Lett 18(14)

      Pages: 4249-52

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Higher in vitro susceptibility of human T cells to H5N1 than H1N1 influenza viruses2008

    • Author(s)
      Li Y-G, Thawatsupha P, Chittaganpitch M, Rungrojcharoenkit K, Li G-M, Nakaya T, Auwanit W, Ikuta K, Sawanpanyalert P
    • Journal Title

      Biochem Biophys Res Commun 371(3)

      Pages: 484-9

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reduced incorporation of SARS-CoV spike protein into viral particles due to amino acid substitutions within the receptor binding domain2008

    • Author(s)
      Li S-M, Li G-M, Nakamura S, Ikuta K, and Nakaya T
    • Journal Title

      Jpn J Infect Dis 61(2)

      Pages: 123-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tumorcidal effect of recombinant Newcastle disease virus2008

    • Author(s)
      Hagiwara K, Kadosawa T, and Nakaya T
    • Journal Title

      The Open Veterinary Science Journal 2

      Pages: 11-5

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells.2008

    • Author(s)
      Daidoji T, Koma T, Du A, Yang C-S, Ueda M, Ikuta K, Nakaya T
    • Journal Title

      J Virol. 82(22)

      Pages: 11294-307

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metagenomic diagnosis of bacterial infections.2008

    • Author(s)
      Nakamura S, Maeda N, Miron I-M, Yoh M, Izutsu K, Kataoka C, Honda T, Yasunaga T, Nakaya T, Kawai J, Hayashizaki Y, Horii T, Iida T
    • Journal Title

      Emerg Infect Dis. 14(11)

      Pages: 1784-86

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus.2008

    • Author(s)
      Ueda M, Yamate M, Du A, Daidoji T, Okuno Y, Ikuta K, Nakaya T
    • Journal Title

      Virus Res. 136(1-2)

      Pages: 91-7

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tumorcidal effect of recombinant Newcastle disease virus.2008

    • Author(s)
      Hagiwara K., Kadosawa T. and Nakaya T.
    • Journal Title

      The Open Veterinary Science Journal 2

      Pages: 11-15

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced incorporation of SARS-CoV spike protein into viral particles due to amino acid substitutions within the receptor binding domain.2008

    • Author(s)
      Li S-M., Li G-M., Nakamura S., Ikuta K. And Nakaya T
    • Journal Title

      Japan Journal of Infectious Disease (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced anti-influenza A virus activity of(-)-epigallocatechin-3-O-gallate fatty acid monoester derivatives: Effect of alkyl chain length.2008

    • Author(s)
      Mori S., Miyake S., Kobe T., Nakaya T; Fuller S-D., Kato N. and Kaihatsu K.
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 次世代型シーケンサを用いた臨床検体からの網羅的なウイルスゲノム検出2008

    • Author(s)
      中屋 隆明
    • Organizer
      第56回 日本ウイルス学会学術集会
    • Place of Presentation
      岡山コンベンションセンター
    • Year and Date
      2008-10-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 高病原性トリインフルエンザウイルスH5N1 は哺乳動物呼吸器上皮細胞に対してアポトーシスを誘導する2008

    • Author(s)
      大道寺智、中屋隆明、他
    • Organizer
      第56回日本ウイルス学会学術集会
    • Place of Presentation
      岡山コンベンションセンター
    • Related Report
      2008 Final Research Report
  • [Presentation] Avian H5N1 influenza virus induces apoptotic cell death in mammalian airway epithelial cells2008

    • Author(s)
      中屋隆明、他
    • Organizer
      第4回日中鳥インフルエンザウイルスシンポジウム
    • Place of Presentation
      中国CDC、北京(中国)
    • Related Report
      2008 Final Research Report
  • [Presentation] 454システムを用いたウイルスゲノムの網羅的解析2007

    • Author(s)
      中屋 隆明
    • Organizer
      第55回 日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2007-10-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Avian H5N1 influenza virus induces apoptotic cell death in mammalian airway epithelial cells2007

    • Author(s)
      中屋隆明、他
    • Organizer
      第55回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2008 Final Research Report
  • [Presentation] Influenza A virus infection in airway epithelial cells2007

    • Author(s)
      中屋隆明
    • Organizer
      第3回日中鳥インフルエンザウイルスシンポジウム
    • Place of Presentation
      ハルビン獣医研究所、ハルビン(中国)
    • Related Report
      2008 Final Research Report
  • [Presentation] 次世代型シーケンサーを用いた臨床検体からの網羅的なウイルスゲノム検出

    • Author(s)
      中屋隆明、他
    • Related Report
      2008 Final Research Report
  • [Presentation] 茶成分エピガロカテキンガレートの化学修飾による抗インフルエンザウイルス活性の向上とその作用機序解明

    • Author(s)
      森修一、中屋隆明、他
    • Related Report
      2008 Final Research Report
  • [Presentation] 鳥インフルエンザウイルス感染時のニワトリ・アヒル線維芽細胞における細胞死誘導機構の比較解析

    • Author(s)
      上田真世、中屋隆明、他
    • Related Report
      2008 Final Research Report
  • [Presentation] 高病原性トリインフルエンザウイルスH5N1の細胞傷害メカニズムの検討

    • Author(s)
      大道寺智、中屋隆明、他
    • Related Report
      2008 Final Research Report
  • [Presentation] 454システムを用いたウイルスゲノムの網羅的解析

    • Author(s)
      中屋隆明、他
    • Related Report
      2008 Final Research Report
  • [Presentation] インフルエンザウイルス感染細胞の小胞体におけるウイルス膜タンパク質の折りたたみ・成熟速度がウイルス粒子産生量に影響を与える

    • Author(s)
      上田真世、中屋隆明、他
    • Related Report
      2008 Final Research Report
  • [Presentation] Genomic deletion and insertion of SARS-CoV by structure-dependent RNA recombination

    • Author(s)
      李樹明、中屋隆明、他
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.biken.osaka-u.ac.jp/

    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] 高病原性トリインフルエンザH5N1亜型ウイルスの検出キット2009

    • Inventor(s)
      中屋隆明、ドゥアナリワ、芝井勇亮、伊藤大輔、岩本久彦
    • Filing Date
      2009-03-01
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] 高病原性トリインフルエンザウイルスに対するモノクローナル抗体2008

    • Inventor(s)
      中屋隆明、ドゥアナリワ、生田和良
    • Filing Date
      2008-05-08
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] 高病原性トリインフルエンザウイルスに対するモノクローナル抗体2007

    • Inventor(s)
      中屋隆明、ドゥアナリワ、生田和良
    • Industrial Property Rights Holder
      財団法人大阪産業振興機構、国立大学法人大阪大学
    • Industrial Property Number
      2007-174587
    • Filing Date
      2007-07-02
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 高病原性トリインフルエンザウイルスに対するモノクローナル抗体

    • Inventor(s)
      中屋隆明、ドゥアナリワ、生田和良
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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