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Chronic infection with Chlamydia pneumoniae

Research Project

Project/Area Number 19591190
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionKawasaki Medical School

Principal Investigator

MIYASHITA Naoyuki  Kawasaki Medical School, 医学部, 講師 (50278917)

Co-Investigator(Kenkyū-buntansha) OUCHI Kazunobu  川崎医科大学, 医学部, 教授 (80351899)
小司 久志  昭和大学, 医学部, 助教 (20412174)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsクラミジア / 慢性持続感染 / プラーク / クローニング / ゲノムタイピング / プラーククローニング / 感染症 / 遺伝子診断
Research Abstract

Chlamydia pneumonia慢性・持続感染の引き起こす病態を明らかとするためには、急性株と慢性株をゲノムタイピングし区別することが必要である。そのため単クローンを精製する目的でプラークの作成を試みた。その結果、世界で初めてC. pneumoniaeのプラーク形成に成功し、クローンを精製することを可能にした。純化した菌株を使用し、C. pneumoniaeに特異的な配列部分を解析したところ各株をグループ化することに成功した。しかし、現時点では正確に鑑別できる部位を特定できておらず、今後は異なったゲノム領域の解析を行っていく予定である。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report

Research Products

(40 results)

All 2009 2008 2007 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (8 results) Book (12 results)

  • [Journal Article] Radiographic features of Mycoplasma pneumoniae pneumonia : differential diagnosis and performance timing2009

    • Author(s)
      Miyashita N, Ouchi K. (他6名, 1番目, 6番目)
    • Journal Title

      BMC Med Imaging 9, 7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development and evaluation of a loop-mediated isothermal amplification method for rapid detection of Chlamydophila pneumoniae2009

    • Author(s)
      Miyashita N
    • Journal Title

      Eur J Clin Microbiol Infect Dis 28(in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid and simple diagnosis of Chlamydophila pneumoniae pneumonia by an immunochromatographic test for detection of immunoglobulin M antibodies2008

    • Author(s)
      Miyashita N, Ouchi K . (他7名, 1番目, 2番目)
    • Journal Title

      Clin Vaccine Immunol 15

      Pages: 1128-1131

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of serological tests for detection of immunoglobulin M antibodies to Chlamydophila pneumoniae2008

    • Author(s)
      Miyashita N, Ouchi K. (他7名, 1番目, 2番目)
    • Journal Title

      Respirology 13

      Pages: 427-431

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of enzyme-linked immunosorbent assay for Chlamydophila pneumoniae-specific immunoglobulin M in acute respiratory tract infection2008

    • Author(s)
      Miyashita N, Ouchi K. (他6名, 1番目, 2番目)
    • Journal Title

      Respirology 13

      Pages: 299-302

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid and simple diagnosis of Chlamydophila pneumoniae pneumonia by an immunochromatographic test for detection of IgM antibodies2008

    • Author(s)
      Miyashita N
    • Journal Title

      Clin Vaccine Immunol 15

      Pages: 1128-1131

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of the usefulness of sputum Gram stain and culture for diagnosis of community-acquired pneumonia requiring hospitalization2008

    • Author(s)
      Miyashita N
    • Journal Title

      Med Sci Monit 14

      Pages: 171-176

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mycoplasma pneumoniae pneumonia in the elderly2008

    • Author(s)
      Miyashita N
    • Journal Title

      Med Sci Monit 14

      Pages: 387-391

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transitional change in the clinical features of pulmonary tuberculosis2008

    • Author(s)
      Miyashita N
    • Journal Title

      Respiration 75

      Pages: 304-309

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Usefulness of the QuantiFERON TB-2G test for the differential diagnosis of pulmonary tuberculosis2008

    • Author(s)
      Miyashita N
    • Journal Title

      Intern Med 47

      Pages: 237-243

    • NAID

      130000079729

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of clinical dosage of gatifloxacin for respiratory tract infections in elderly patients based on pharmacokinetics/pharmacodynamics (PK/PD)2008

    • Author(s)
      Miyashita N
    • Journal Title

      J Infect Chemother 14

      Pages: 296-304

    • NAID

      10022595471

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An asymptomatic case of pulmonary cryptococcosis with endobronchial polypoid lesions and bilateral infiltrative shadow2008

    • Author(s)
      Miyashita N
    • Journal Title

      J Infect Chemother 14

      Pages: 315-318

    • NAID

      10022595534

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical utility of the QuantiFERON TB-2G test for elderly patients with active tuberculosis2008

    • Author(s)
      Miyashita N
    • Journal Title

      Chest 133

      Pages: 1196-1202

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical evaluation for diagnosing active TB disease and transitional change of two commercial blood tests2008

    • Author(s)
      Miyashita N
    • Journal Title

      Scand J Infect Dis 40

      Pages: 629-634

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term follow-up of the QuantiFERON TB-2G test for active tuberculosis disease2008

    • Author(s)
      Miyashita N
    • Journal Title

      Intern Med 47

      Pages: 1957-1961

    • NAID

      130000079814

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pulmonary mycosis (Cunninghamella bertholletiae) with cavitation diagnosed using Ultra-thin fibre-optic bronchoscopy2008

    • Author(s)
      Miyashita N
    • Journal Title

      Respirology 13

      Pages: 312-314

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of enzyme linked immunosorbent assay for Chlamydophila pneumoniae-specific immunoglobulin M in acute respiratory tract infection2008

    • Author(s)
      Miyashita N.
    • Journal Title

      Respirology 13

      Pages: 299-302

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of serological tests for detection of immunoglobulin M anfibodies to Chlamydophila pneumoniae2008

    • Author(s)
      Miyashita N.
    • Journal Title

      Respirology 13

      Pages: 427-431

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of diagnostic usefulness of real-time PCR for detection of Chlamydophila pneumoniae in acute respiratory infections2007

    • Author(s)
      Miyashita N.
    • Journal Title

      J.Infect.Chemother. 13

      Pages: 183-187

    • NAID

      10019548418

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development and evaluation of a loop-mediated isothermal amplification method for rapid detection of Chlamydophila pneumoniae

    • Author(s)
      Kawai Y, Miyashita N, Ouchi K. (他6名, 2番目, 9番目)
    • Journal Title

      Eur J Clin Microbiol Infect Dis 2009 (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] Rapid diagnosis of Chlamydia pneumoniae pneumonia by using an immunochromatographic test for the detection of immunoglobulin M antibodies2008

    • Author(s)
      Miyashita N, Ouchi K. (他6名, 1番目, 2番目)
    • Organizer
      6th Meeting of the European Society for Chlamydia Research
    • Place of Presentation
      Denmark
    • Year and Date
      2008-07-01
    • Related Report
      2008 Final Research Report
  • [Presentation] Plaque formation of Chlamydophila pneumoniae2008

    • Author(s)
      Miyashita N, Ouchi K. (他8名, 1番目, 9番目)
    • Organizer
      6th Meeting of the European Society for Chlamydia Research
    • Place of Presentation
      Denmark
    • Year and Date
      2008-07-01
    • Related Report
      2008 Final Research Report
  • [Presentation] Rapid diagnosis of Chlamydophila pneumoniae pneumonia by using an immunochromatographic test for detection of IgM antibodies2008

    • Author(s)
      Miyashita N
    • Organizer
      Sixth meeting of the European society for Chlamydia research
    • Place of Presentation
      Denmark
    • Year and Date
      2008-07-01
    • Related Report
      2008 Annual Research Report
  • [Presentation] Plaque formation of Chlamydophila pneumoniae2008

    • Author(s)
      Miyashita N
    • Organizer
      Sixth meeting of the European society for Chlamydia research
    • Place of Presentation
      Denmark
    • Year and Date
      2008-07-01
    • Related Report
      2008 Annual Research Report
  • [Presentation] Comparison of serological tests for detection of immunoglobulin M antibodies to Chlamydia pneumonia2007

    • Author(s)
      Miyashita N, Ouchi K. (他6名, 1番目, 2番目)
    • Organizer
      12th Congress of the Asian Pacific Society of Respirology/2nd Joint Congress of the APSR/ACCP
    • Place of Presentation
      Australia
    • Year and Date
      2007-12-01
    • Related Report
      2008 Final Research Report
  • [Presentation] Clinical features of severe Mycoplasma pneumoniae in adults admitted to an intensive care unit2007

    • Author(s)
      Miyashita N, Ouchi K. (他8名, 1番目, 9番目)
    • Organizer
      12th Congress of the Asian Pacific Society of Respirology/2nd Joint Congress of the APSR/ACCP
    • Place of Presentation
      Australia
    • Year and Date
      2007-12-01
    • Related Report
      2008 Final Research Report
  • [Presentation] Comparison of serological tests for detection of immunoglobulin M antibodies to Chlamydophila pneumoniae2007

    • Author(s)
      Miyashita N.
    • Organizer
      The 12th Congress of the Asian Pacific Society ofRespirology/2nd Joint Congress of the APSR/ACCP
    • Place of Presentation
      Australia
    • Year and Date
      2007-12-01
    • Related Report
      2007 Annual Research Report
  • [Presentation] Establishment of a lamp for rapid diagnostic method of Chlamydia pneumoniae2007

    • Author(s)
      Kawai Y, Ouchi K , Miyashita N. (他4名, 2番目, 5番目)
    • Organizer
      5th World Congress of the World Society for Pediatric Infectious Diseases
    • Place of Presentation
      Thailand
    • Year and Date
      2007-11-17
    • Related Report
      2008 Final Research Report
  • [Book] クラミドフィラ肺炎の臨床診断.肺炎の画像診断と最新の診療, 藤田次郎 編, 210-2172008

    • Author(s)
      宮下修行
    • Publisher
      医薬ジャーナル社
    • Related Report
      2008 Final Research Report
  • [Book] マイコプラズマ・クラミジア.感染症内科クリニカルスタンダード, 前崎繁文 編, 196-1982008

    • Author(s)
      宮下修行, 杉生忠昭, 清水大樹, 岡三喜男
    • Publisher
      文光堂
    • Related Report
      2008 Final Research Report
  • [Book] クラミジア・ニューモニエ.KEY WORD 感染症第2版, 山口惠三, 戸塚恭一 編, 48-492008

    • Author(s)
      宮下修行
    • Publisher
      先端医学社
    • Related Report
      2008 Final Research Report
  • [Book] 成人院内肺炎診療ガイドライン2008

    • Author(s)
      宮下修行
    • Total Pages
      72
    • Publisher
      社団法人日本呼吸器学会
    • Related Report
      2008 Annual Research Report
  • [Book] 肺炎の画像診断と最新の診療2008

    • Author(s)
      宮下修行
    • Total Pages
      8
    • Publisher
      医薬ジャーナル社
    • Related Report
      2008 Annual Research Report
  • [Book] KEY WORD感染症第2版2008

    • Author(s)
      宮下修行
    • Total Pages
      2
    • Publisher
      先端医学社
    • Related Report
      2008 Annual Research Report
  • [Book] 感染症内科クリニカルスタンダードー必携ベッドサイドで必ず役立つ臨床感染症学のエッセンス2008

    • Author(s)
      宮下修行
    • Total Pages
      3
    • Publisher
      文光堂
    • Related Report
      2008 Annual Research Report
  • [Book] 腹痛診療ナビ2008

    • Author(s)
      宮下修行
    • Total Pages
      6
    • Publisher
      日本医事新報社
    • Related Report
      2008 Annual Research Report
  • [Book] 今日の耳鼻咽喉科頭頚部外科治療指針2008

    • Author(s)
      宮下修行
    • Total Pages
      1
    • Publisher
      医学書院
    • Related Report
      2008 Annual Research Report
  • [Book] クラミジア肺炎.呼吸器専門医テキスト, 工藤翔二, 中田紘一郎, 永井厚志, 大田健 編, 325-3272007

    • Author(s)
      宮下修行, 岡三喜男
    • Publisher
      南江堂
    • Related Report
      2008 Final Research Report
  • [Book] クラミジアニューモニエ肺炎.呼吸器疾患最新の治療2007-2009, 工藤翔二, 中田紘一郎, 貫和敏博 編, 229-2312007

    • Author(s)
      宮下修行, 岡三喜男
    • Publisher
      南江堂
    • Related Report
      2008 Final Research Report
  • [Book] 呼吸器専門医テキスト2007

    • Author(s)
      宮下 修行
    • Total Pages
      8
    • Publisher
      南江堂
    • Related Report
      2007 Annual Research Report

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Published: 2007-03-31   Modified: 2016-04-21  

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