RNA interference-based therapeutic molecular targeting strategy using nano drug delivery systems to eradicate malignant melanoma

• Project/Area Number: 19591323
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SABURO Kishimoto (2008) Kyoto Prefectural University of Medicine, 医学研究科, 教授 (90079913); 浅田 秀基 (2007) Kyoto Prefectural University of Medicine, 医学部, 博士研究員 (20399041)
• Co-Investigator(Kenkyū-buntansha): 岸本 三郎 京都府立医科大学, 医学研究科, 教授 (90079913)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 悪性黒色腫 / 分子標的治療 / RNAインタフェレンス / サイトカイン / 免疫療法 / RNAi / DDS / 癌 / 免疫学

Research Abstract

我々は最近、メラノーマに発現するbHLH転写因子、MITFをRNAiにて抑制すると、メラノーマがアポトーシスに陥り、増殖が阻止できることを見出した。本研究では、さらなる発展として、IL-12免疫遺伝子療法との併用により、それらの相乗作用によるより強力なメラノーマの抑制に成功した。さらに、ナノDDSによるsiRNA導入を試みるとともに、膜透過性ペプチドによるMITF特異的分子標的療法の試みを行った。

Research Products

• [Journal Article] Immunoregulatory T cells in the peripheral blood of melanoma patients treated with melanoma antigen-pulsed mature monocyte-derived dendritic cell vaccination (2009)
  • Author(s): Nakai N, Katoh N, Kitagawa T, Ueda E, Takenaka H, Kishimoto S
  • Journal Title: J Dermatol Sci 54 (1) Pages: 31-37
  • Peer Reviewed
• [Journal Article] Immunohistological analysis of peptide-induced delayed-type hypersensitivity in advanced melanoma patients treated with melanoma antigen-pulsed mature monocyte-derived dendritic cell vaccination (2009)
  • Author(s): Nakai N, Katoh N, Germeraad WT, Kishida T, Ueda E, Takenaka H, Mazda O, Kishimoto S
  • Journal Title: J Dermatol Sci 53(1) Pages: 40-47
  • Peer Reviewed
• [Journal Article] Immunoregulatory T cells in the peripheral blood of melanoma patients treated with melanoma antigen-pulsed mature monocyte-derived dendritic cell vaccination (2009)
  • Author(s): Nakai N, Katoh N, Kitagawa T, Ueda E, Takenaka H, Kishimoto S.
  • Journal Title: J Dermatol Sci. 54(1) Pages: 31-37
  • Peer Reviewed
• [Journal Article] Immunohistological analysis of peptide-induced delayed-type hyperse nsitivity in advanced melanoma patients treated with melanoma antigen-pulsed mature monocyte-derived dendritic cell vaccination (2009)
  • Author(s): Nakai N, Katoh N, Germeraad WT, Kishida T, Ueda E, Takenaka H, Mazda 0, Kishimoto S.
  • Journal Title: J Dermatol Sci. 53(1) Pages: 40-47
  • Peer Reviewed
• [Journal Article] Evaluation of survival in Japanese stage IV melanoma patients treated with melanoma antigen-pulsed mature monocyte-derived dendritic cells (2008)
  • Author(s): Nakai N, Katoh N, Kitagawa T, Ueda E, Takenaka H, Kishimoto S
  • Journal Title: J Dermatol 35(12) Pages: 801-3
  • Peer Reviewed
• [Journal Article] Pleiotrophic Functions of Epstein-Barr Virus Nuclear Antigen-1 (EBNA-1) and oriP Differentially Contribute to the Efficacy of Transfection/expression of Exogenous Gene in Mammalian Cells (2008)
  • Author(s): Kishida, T., H. Asada, K. Kubo, Y. Sato, M. Shin-Ya, J. Imanishi, K. Yoshikawa & O. Mazda
  • Journal Title: J. Biotechnol 133 (2) Pages: 201-207
  • Peer Reviewed
• [Journal Article] Pleiotrophic functions of Epstein-Barr virus nuclear antigen-1(EBNA-1)and oriP differentially contribute to the efficiency of transfection/expression of exogenous gene in mammalian cells. (2008)
  • Author(s): Kishida, T., et. al.
  • Journal Title: J. Biotechnol. 133 Pages: 201-207
  • Peer Reviewed
• [Journal Article] IL-21 induces inhibitor of differentiation 2 (Id2) and leads to complete abrogation of anaphylaxis in mice (2007)
  • Author(s): Kishida, T., Y. Hiromura, M. Shin-Ya, H. Asada, H. Kuriyama, M. Sugai, A. Shimizu, Y. Yokota, T. Hama, J. Imanishi, Y. Hisa & O. Mazda
  • Journal Title: J. Immunol 179 (12) Pages: 8554-8561
  • Peer Reviewed
• [Journal Article] IL-21 induces inhibitor of differentiation 2 and leads to complete abrogation of anaphylaxis in mice. (2007)
  • Author(s): Kishida, T., et. al.
  • Journal Title: J. Immunol. 179 Pages: 8554-8561
  • Peer Reviewed
• [Presentation] Double Cancer of Planter Malingnant Melanoma and Vulvar Extramammary Paget's Disease (2008)
  • Author(s): Tsuji M, Nakai N, Ueda E, Takenaka H, Katoh N, Kishimoto S
  • Organizer: The 10th China-Japan Joint Meeting of Dermatology
  • Place of Presentation: Hangzhou, China