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The comprehensive analysis of human cancer tissue by ChIP/Ologofishmethods

Research Project

Project/Area Number 19591573
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionHokkaido University

Principal Investigator

NAKANISHI Kazuaki  Hokkaido University, 大学院・医学研究科, 特任助教 (80374338)

Co-Investigator(Kenkyū-buntansha) OZAKI Michitaka  北海道大学, 大学院・医学研究科, 特任教授 (80256510)
藤堂 省  北海道大学, 大学院・医学研究科, 教授 (60136463)
Co-Investigator(Renkei-kenkyūsha) TODO Satoru  北海道大学, 大学院・医学研究科, 教授 (60136463)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsクロマチン免疫沈降法 / 肝再生 / 肝癌 / STAT3 / PDK1 / Akt / 肝細胞増殖
Research Abstract

マウスによるChIP法を応用した転写因子のターゲット遺伝子、機能解析を行った。肝特異的KOマウスSTAT3-KOマウスでは肝細胞増殖はほぼ完全に抑制されていたが、PDK1/Akt経路の代償性の活性化により肝再生は維持された。活性型STAT3の導入により、肝細胞分裂が誘導された。ChIP法により、STAT3の新たなターゲットとしてNet遺伝子を見出した。Netは、肝細胞にてSTAT3により制御されており、肝再生に影響を与えていた。STAT3は、CyclinD1、Myc、Netなどを制御し、肝再生に関わっていた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (33 results)

All 2009 2008 2007

All Journal Article (16 results) (of which Peer Reviewed: 15 results) Presentation (17 results)

  • [Journal Article] The survival pathways phosphatidylinositol-3 kinase (PI3-K)/phosphoinositide-dependent protein kinase 1 (PDK1)/Akt modulate liver regeneration through hepatocyte size rather than proliferation2009

    • Author(s)
      Sanae Haga, Michitaka Ozaki, Hiroshi Inoue, Yasuo Okamoto, Wataru Ogawa, Kiyoshi Takeda, Shizuo Akira, Satoru Todo
    • Journal Title

      Hepatology 49

      Pages: 204-214

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nobuhiro Ohkohchi. Autonomic regulation of liver regeneration after partial hepatectomy in mice2008

    • Author(s)
      Osamu Ikeda, Michitaka Ozaki, Soichiro Murata, Ryota Matsuo, Yoritaka Nakano, Motonobu Watanabe, Katsuji Hisakura, Andriy Myronovych
    • Journal Title

      J Surg Res 152(2)

      Pages: 218-223

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deficient response of IL-6 impaired liver regeneration after hepatectomy in patients with viral hepatitis2008

    • Author(s)
      N. Gotohda, H. Iwagaki, M. Ozaki, T. Kinoshita, M. Konishi, T. Nakagohri, S. Takahashi, S. Saito, T. Yagi, N. Tanakaq
    • Journal Title

      Hepato-Gastroenterology 55(85)

      Pages: 1439-44

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The significant correlation between surgical stress of hepatectomy and changes in the serum levels of HGF, IL-6 and soluble Fas in the patients with viral hepatitis2008

    • Author(s)
      Naoto Gotohda, Hiromi Iwagaki, Michitaka Ozaki, Taira Kinoshita, Masaru Konishi, Toshio Nakagohri, Shinichiro Takahashi, Shinya Saito, Takahito Yagi, Noriaki Tanaka
    • Journal Title

      Hepato-Gastroenterology 55(85)

      Pages: 1400-3

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Blot GlycoABCTM : An integrated glycoblotting technique for rapid and large-scale clinical glycomics2008

    • Author(s)
      Yoshiaki Miura, Magumi Hato, Yasuro Shinohara, Hiromitsu Kuramoto, Jun-ichi Furukawa, Masaki Kurogochi, Hideyuki Shimaoka, Mitsuhiro Tada, Kazuaki Nakanishi, Michitaka Ozaki, Satoru Todo, Shin-Ichiro Nishimura
    • Journal Title

      Mol Cell Proteomics 7(2)

      Pages: 370-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of de novo STAT3 target gene in liver regeneration2008

    • Author(s)
      Hui-qi Zhang, Sanae Haga, Moto Fukai, Yuko Oikawa, Hiroshi Inoue, Wataru Ogawa, Arihiko Kano, Atsushi Maruyama, Xin-Yuan Fu, Hiroyuki Furukawa, Satoru Todo, Shin Enosawa, Michitaka Ozaki
    • Journal Title

      Hepatol Res 38(4)

      Pages: 374-84

    • NAID

      10024147922

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preventing hypoxia/reoxygenation damage to hepatocytes by p66SHC ablation : up-regulation of anti-oxidant and anti-apoptotic proteins2008

    • Author(s)
      Sanae Haga, Keita Terui, Moto Fukai, Yuko Oikawa, Kaikobad Irani, Hiroyuki Furukawa, Satoru Todo, Michitaka Ozaki
    • Journal Title

      J Hepatol 48

      Pages: 422-432

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 肝障害・再生のメカニズム-Jak/STAT3およびPI3-K/Akt経路の役割と意義-2008

    • Author(s)
      尾崎 倫孝
    • Journal Title

      生化学 80(5)

      Pages: 399-408

    • NAID

      10021191847

    • Related Report
      2008 Final Research Report
  • [Journal Article] Autonomic regulation of liver regeneration after partial hepatectomy in mice2008

    • Author(s)
      Osamu Ikeda
    • Journal Title

      Journal of Surgical Research 152

      Pages: 218-223

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The survival pathways phosphatidylinositol-3 kinase (PI3-K)/phosphoinositide-dependent protein kinase 1 (PDK1)/Akt modulate liver regeneration through hepatocyte size rather than proliferation2008

    • Author(s)
      Sanae Haga
    • Journal Title

      Hepatology 49

      Pages: 204-214

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficient response of IL-6 impaired liver regeneration after hepatectomy in patients with viral hepatitis2008

    • Author(s)
      Naoto Gotohda
    • Journal Title

      Hepatogastroenterology 55

      Pages: 1439-1444

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The significant correlation between surgical stress of hepatectomy and changes in the serum levels of HGF, IL-6 and soluble Fas in the patients with viral hepatitis2008

    • Author(s)
      Naoto Gotohda
    • Journal Title

      Hepatogastroenterology 55

      Pages: 1400-1403

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BlotGlyco ABCTM : An integrated glycoblotting technique for rapid and large-scale clinical glycomics2008

    • Author(s)
      Yoshiaki Miura
    • Journal Title

      Molcular & Cellular Proteomics 7

      Pages: 370-377

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of de novo STAT3 target gene in liver regeneration2008

    • Author(s)
      Hui-qi Zhang
    • Journal Title

      Hepatology Research 38

      Pages: 374-384

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preventing hypoxia/reoxygenation damage to hepatocytes by p66slic ablation : up-regulation of anti-oxidant and anti-apoptotic proteins2008

    • Author(s)
      Sanae Haga
    • Journal Title

      Journal of Hepatology 48

      Pages: 422-432

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 肝障害・再生のメカニズム-Jak/STAr3およびPI3-K/Akt経路の役割と意義-2008

    • Author(s)
      尾崎倫孝
    • Journal Title

      生化学 80

      Pages: 399-408

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] #1 molecular mechanism of liver injury, protection and regeneration, #2 bio-imaging of molecular functions and tumor by optic probes2009

    • Author(s)
      Michitaka Ozaki.
    • Organizer
      Japan-Denmark Joint Workshop on "Molecular Cancer Research"
    • Place of Presentation
      Tokyo
    • Related Report
      2008 Final Research Report
  • [Presentation] #1 molecular mechanism of liver injury, protection and regeneration, #2 bio-imaging of molecular functions and tumor by optic probes2009

    • Author(s)
      Michitaka Ozaki
    • Organizer
      Japan-Denmark Joint Workshop on "Molecular Cancer Research"
    • Place of Presentation
      東京(六本木ヒルズ)
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肝の障害と再生のメカニズム-Jak/STAT3およびPI3-K/PDK1/Akt経路の役割と意義-2008

    • Author(s)
      尾崎倫孝
    • Place of Presentation
      東京大学医科学研究所、東京
    • Year and Date
      2008-11-14
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝の障害と再生のメカニズム-Jak/STAr3およびPI3-K/PDK1/Akt経路の役割と意義-2008

    • Author(s)
      尾崎倫孝
    • Organizer
      東京大学医科学研究所セミナー
    • Place of Presentation
      東京(東京大学医科学研究所)
    • Year and Date
      2008-11-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] 「北海道大学第一外科における組織バンクの構築と解析の実際」シンポジウム22「バイオマーカー研究最前線」2008

    • Author(s)
      尾崎倫孝、高橋将人、藤堂省
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-28
    • Related Report
      2008 Final Research Report
  • [Presentation] 北海道大学第一外科における組織バンクの構築と解析の実際2008

    • Author(s)
      尾崎倫孝
    • Organizer
      第67回日本癌学会学術総会、シンポジウム22「バイオマーカー研究最前線」
    • Place of Presentation
      名古屋(名古屋国際会議場)
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 生体光イメージング法による臓器移植の新たな展開-分子機能イメージングによるグラフト機能評価・予後予測の可能性-2008

    • Author(s)
      芳賀早苗
    • Organizer
      第44回日本移植学会総会
    • Place of Presentation
      大阪(大阪国際会議場)
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肝再生シグナルの解析と再生不全の要因-肝切除後再生時の肝細胞増殖・成長・アポトーシスの責任シグナルと肝再生への影響-2008

    • Author(s)
      尾崎倫孝、芳賀早苗、井上啓、小川渉、村田宏、岩垣博巳、藤堂省
    • Organizer
      第15回肝細胞研究会
    • Place of Presentation
      静岡
    • Year and Date
      2008-06-27
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝再生シグナルの解析と再生不全の要因-肝切除後再生時の肝細胞増殖・成長・アポトーシスの責任シグナルと肝再生への影響-2008

    • Author(s)
      尾崎倫孝
    • Organizer
      第15回肝細胞研究会、シンポジウム
    • Place of Presentation
      静岡(静岡県男女共同参画センター「あざれあ」)
    • Year and Date
      2008-06-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 加齢肝の再不全におけるp66^<snc>の関与とその機序2008

    • Author(s)
      芳賀早苗
    • Organizer
      第15回肝細胞研究会
    • Place of Presentation
      静岡(静岡県男女共同参画センター「あざれあ」)
    • Year and Date
      2008-06-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 高解像度BAC array CGH解析により抽出された胃癌の予後に関連するゲノム異常領域2008

    • Author(s)
      富岡伸元
    • Organizer
      第12回がん分子標的治療研究会総会
    • Place of Presentation
      東京(学術総合センター)
    • Year and Date
      2008-06-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] プロテオーム解析による肝細胞癌の早期再発マーカーの探索2008

    • Author(s)
      横尾英樹
    • Organizer
      第12回がん分子標的治療研究会総会
    • Place of Presentation
      東京(学術総合センター)
    • Year and Date
      2008-06-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 生体光イメージング法による臓器移植の新たな展開-分子機能イメージングによるグラフト機能評価・予後予測の可能性-2008

    • Author(s)
      芳賀 早苗、及川 優子、小澤岳昌、James Remington、古川博之、藤堂省、尾崎倫孝
    • Organizer
      第44回日本移植学会総会
    • Place of Presentation
      大阪
    • Related Report
      2008 Final Research Report
  • [Presentation] 加齢肝の再生不全におけるp66shcの関与とその機序2008

    • Author(s)
      芳賀早苗、及川優子、藤堂省、尾崎倫孝
    • Organizer
      第15回肝細胞研究会
    • Place of Presentation
      静岡
    • Related Report
      2008 Final Research Report
  • [Presentation] 高解像度BAC array CGH解析により抽出された胃癌の予後に関連するゲノム異常領域2008

    • Author(s)
      富岡 伸元、齋藤総一郎、多田光宏、高橋典彦、片岡昭彦、中西一彰、高橋将人、尾崎倫孝、平野 隆、藤堂省
    • Organizer
      第12回がん分子標的治療研究会総会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] プロテオーム解析による肝細胞癌の早期再発マーカーの探索2008

    • Author(s)
      横尾英樹、近藤格、中西一彰、神山俊哉、尾崎倫孝、藤堂省、廣橋説雄
    • Organizer
      第12回がん分子標的治療研究会総会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 「肝再生シグナルの解析と再生不全の要因-肝切除後再生時の肝細胞増殖・成長・アポトーシスの責任シグナルと肝再生への影響-2007

    • Author(s)
      尾崎倫孝、芳賀早苗、五十嵐瑠美、長田忠大、井上啓、小川渉、村田宏、岩垣博巳、古川博之、藤堂省
    • Organizer
      第14回肝細胞研究会
    • Place of Presentation
      鹿児島 城山観光ホテル
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2021-12-08  

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