The role of serotonin 2C receptor RNA editing in the neuropathic pain model

• Project/Area Number: 19591791
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Osaka University
• Principal Investigator: TAKASHINA Masaki Osaka University, 医学部附属病院, 講師 (30221352)
• Co-Investigator(Kenkyū-buntansha): HAGIHIRA Satoshi 大阪大学, 医学部附属病院, 講師 (90243229) ; NAKAE Aya 大阪大学, 医学部附属病院, 特任准教授 (60379170)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: セロトニン2C受容体 / RNA編集 / 神経因性疼痛モデル / 脊髄障害性モデル / 脊髄障害性疼痛 / 脊髄障害性疼痛モデル / 核小体低分子RNA / RBII-52 / SCI / 選択的スプライシング / 脊髄坐滅モデル / 神経因性疼痛

Research Abstract

We investigated the effects on 5HT2C receptor RNA editing efficiency of contusive SCI. Using cloning followed by sequence analysis on spinal cord samples takes, we compared mRNA editing. Our results might be evidence of functional adaptation mechanism in which increased expression of 5HT2C mRNA isoforms that encode receptors more sensitive to serotonin works to activate brainstem-spinal descending 5HT systems to, in effect, suppress transmission of nociceptive signals from primary afferent neurons to the spinal dorsal horn.

Research Products

• [Journal Article] The role of snoRNA RBII-52 to the serotonin2C receptor in the rat oro-facial neuropathic pain model (2009)
  • Author(s): Kunihiro Nakai, Aya Nakae, Sosuke Oba, Masahiko Shibata, Takashi Mashimo, Koichi Ueda
  • Journal Title: Neuroscience
• [Journal Article] The snoRNA RBII-52 regulates alternative splicing of serotonin 2C receptor in the rat oro-facial neuropathic pain model (2009)
  • Author(s): Aya Nakae, Kunihiro Nakai, Tatsuya Tanaka, Mari Yoshida, Akiko Mikami, Masaki Takashina, Satoshi Hagihira, Masahiko Shibata, Koichi Ueda, Takashi Mashimo
  • Journal Title: Neuroscience
• [Journal Article] The role of snoRNA(RBII-52) in a rat model of oro-facial neuropathic pain (2009)
  • Author(s): A. Nakae, K. Nakai, T. Tanaka, S. Hagihira, T. Mashimo
  • Journal Title: European Journal of Anesthesiology 26 Pages: 14AP4-2
• [Journal Article] Role of constitutively active 5HT2C receptors following spinal cord injury in rats (2008)
  • Author(s): K.C. Murray, A. Nakae, T. Ballou, R. Vavrek, K. Fouad, M.M. Rank, M. Stephens, R. Anelli, P.J. Harvey, C. Heckman, D.J. Bennett
  • Journal Title: Neuroscience
• [Journal Article] The role of RNA editing of the serotonin 2C receptor in a rat model of oro-facial neuropathic pain (2008)
  • Author(s): Aya Nakae, Kunihiro Nakai, Tatsuya Tanaka, Satoshi Hagihira, Masahiko Shibata, Koichi Ueda, Takashi Mashimo
  • Journal Title: The European Journal of Neuroscience 27(9) Pages: 2373-9
• [Journal Article] Serotonin 2C receptor mRNA editing in neuropathic pain model (2008)
  • Author(s): Aya Nakae, Kunihiro Nakai, Tatsuya Tanaka, Satoshi Hagihira, Masahiko Shibata, Koichi Ueda, Takashi Mashimo
  • Journal Title: Neuroscience Research 60(2) Pages: 228-231
• [Journal Article] The role of RNA editing of the serotonin 2C receptor in a rat model of oro-facial neuropathic pain. (2008)
  • Author(s): Aya Nakae, Kunihiro Nakai, Takashi Mashimo, Et al.
  • Journal Title: European Journal of Nesuroscience 27 Pages: 2373-2379
  • Peer Reviewed
• [Journal Article] The role of RNA editing of serotonin2C receptor in a rat model of oro-facial neuropathic pain (2007)
  • Author(s): A. Nakae, K. Nakai, T. Tanaka, K. Miyake, S. Hagihira, M. Shibata, K. Ueda, T. Mashimo
  • Journal Title: Neuroscience
• [Journal Article] Serotonin2C receptor mRNA editing in neuropathic pain model (2007)
  • Author(s): Aya Nakae, Takashina Masaki
  • Journal Title: Neuroscience Research 60-2 Pages: 228-231
  • Peer Reviewed
• [Presentation] 核小体RNA、RBII-52の神経因性疼痛でのセロトニン2C受容体に対する役割 (2009)
  • Author(s): 吉田真理、田中達哉、石田葉子、中江文、中井國博、三上晶子、大場創介、柴田政彦、眞下節、上田晃一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] 核小体RNAのRBII-52によるラット顔面神経因性疼痛モデルにおけるセロトニン2Cレセプターのスプライスバリアントの制御 (2009)
  • Author(s): 三上晶子、田中達哉、石田葉子、中江文、中井國博、吉田真理、柴田政彦、上田晃一、眞下節
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] The role of snoRNA RBII-52 to the serotonin2C receptor in the rat oro-facial neuropathic pain model (2009)
  • Author(s): Kunihiro Nakai, Aya Nakae, Sosuke Oba, Masahiko Shibata, Takashi Mashimo, Koichi Ueda
  • Organizer: Neuroscience 2009
  • Place of Presentation: Chicago, Ilinoi
  • Year and Date: 20091017-20091021
• [Presentation] The snoRNA RBII-52 regulates alternative splicing of serotonin 2C receptor in the rat oro-facial neuropathic pain model (2009)
  • Author(s): Aya Nakae, Kunihiro Nakai, Tatsuya Tanaka, Mari Yoshida, Akiko Mikami, Masaki Takashina, Satoshi Hagihira, Masahiko Shibata, Koichi Ueda, Takashi Mashimo
  • Organizer: Neuroscience 2009
  • Place of Presentation: Chicago, Ilinoi
  • Year and Date: 20091017-20091021
• [Presentation] 眼窩下神経絞扼モデルにおける核小体RNA(RBII-52)の関わり (2009)
  • Author(s): 中江文、中井國博、柴田政彦、上田幸一、眞下節
  • Organizer: 第31回日本疼痛学会
  • Place of Presentation: 名古屋
  • Year and Date: 20090717-20090718
• [Presentation] The role of snoRNA in a rat model of oro-facial neuropathic pain (2009)
  • Author(s): Aya Nakae, Kunihiro Nakai, Tatsuya Tanaka, Satoshi Hagihira, Takashi Mashimo
  • Organizer: Euroanaesthesia 2009
  • Place of Presentation: Milano, Italy
  • Year and Date: 20090606-20090609
• [Presentation] The role of GABAergic tonic inhibition in rat oro-facial neuropathic pain model (2009)
  • Author(s): Kunihiro Nakai, Aya Nakae, Sosuke Oba, Takashi Mashimo, Koichi Ueda
  • Organizer: 5th World Congress World Institute of Pain
  • Place of Presentation: New York, NY
  • Year and Date: 20090313-20090316
• [Presentation] Correlation between peripheral P2X4 receptor expression and nerve injury in rat oro-facial neuropathic pain model (2009)
  • Author(s): Aya Nakae, Kunihiro Nakai, Tatsuya Tanaka, Koichi Ueda, Takashi Mashimo
  • Organizer: 5th World Congress World Institute of Pain
  • Place of Presentation: New York, NY
  • Year and Date: 20090313-20090316
• [Presentation] 脊髄におけるセロトニン2C受容体のRNA編集とその病態へのかかわり (2009)
  • Author(s): 中江文、柴田政彦、高階雅紀、萩平哲、眞下節
  • Organizer: 平成20年度生理学研究所研究会「筋・骨格系と内臓の痛み研究会」
  • Place of Presentation: 岡崎
  • Year and Date: 20090122-20090123
• [Presentation] 神経因性疼痛モデルでの核小体RNA(RBII-52)の関与 (2009)
  • Author(s): 吉田真理, 中江文, ほか
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] The snoRNA RBII-52 regulates alternative splicing of serotonin 2C receptor in the rat oro-facial neuropathic pain model (2009)
  • Author(s): Aya Nakae, Masaki Takashina, 他
  • Organizer: Neuroscience 2009
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-14
• [Presentation] Role of constitutively active 5HT2C receptors following spinal cord injury in rats (2008)
  • Author(s): K.C. Murray, A. Nakae, T. Ballou, R. Vavrek, K. Fouad, M.M. Rank, M. Stephens, R. Anelli, P.J. Harvey, C. Heckman, D.J. Bennett
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washington, DC
  • Year and Date: 20081115-20081119
• [Presentation] The role of RNA editing of serotonin2C receptor in a rat model of oro-facial neuropathic pain (2007)
  • Author(s): A. Nakae, K. Nakai, T. Tanaka, K. Miyake, S. Hagihira, M. Shibata, K. Ueda, T. Mashimo
  • Organizer: Neuroscience 2007
  • Place of Presentation: San Diego, CA
  • Year and Date: 20071103-20071107
• [Presentation] 神経因性疼痛におけるセロトニン2C受容体の関与 (2007)
  • Author(s): 中江文、田中達哉、高階雅紀、上山博史、萩平哲、眞下節
  • Organizer: 日本麻酔科学会第54回学術集会
  • Place of Presentation: 札幌
  • Year and Date: 20070531-20070602
• [Presentation] 神経因性疼痛におけるセロトニン2C受容体の関与 (2007)
  • Author(s): 中江 文、高階 雅紀、萩平 哲
  • Organizer: 日本麻酔科学会第54回学術集会
  • Place of Presentation: 札幌コンヴェンションセンター
  • Year and Date: 2007-06-01