Molecular mechanisms of the suppression of invasion and metastasis by periostin in urothelial carcinoma

• Project/Area Number: 19591843
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: KIM Chul Jang Shiga University of Medical Science, 医学部, 非常勤講師 (10204968)
• Co-Investigator(Kenkyū-buntansha): INOUE Hirokazu 滋賀医科大学, 医学部, 准教授 (30176440) ; ISONO Takahiro 滋賀医科大学, 医学部, 准教授 (20176259)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 尿路上皮癌 / periostin / 浸潤 / 転移 / 癌抑制遺伝子

Research Abstract

Periostinによる膀胱癌細胞の浸潤・転移抑制の分子機構を解明するために、免疫沈降法と質量分析計を用いて、 Periostinと結合する蛋白質としてMAP キナーゼファミリーに属するTAK1の活性化因子であるTAB1を同定した。さらに、TAB1およびTAK1 のsiRNAノックダウン実験などにより、Periostin による膀胱癌細胞の浸潤能の抑制にはTAB1/TAK1 シグナル経路が重要な働きをしていることを明らかにした

Research Products

• [Journal Article] A comparison of management of hemorrhage following transurethral resection of the prostate between routine continuous bladder irrigation and no bladder irrigation with catheter traction : A prospective randomized study (2009)
  • Author(s): Kim CJ, Takimoto K, Tomita K, Osafune T
  • Journal Title: Jpn J Endourol ESWL 22 Pages: 88-91
  • Peer Reviewed
• [Journal Article] Downregulation of drs tumor suppressor gene in highly malignant human pulmonary neuroendocrine tumors (2009)
  • Author(s): Shimakage M, Kodama K, Kawahara K, Kim CJ, Ikeda K, Yutsudo M, Inoue H
  • Journal Title: Oncol Rep 21 Pages: 1367-1372
  • Peer Reviewed
• [Journal Article] Cyclin D1 downregulation is important for permanent cell cycle exit and initiation of differentiation induced by anchorage-deprivation in human keratinocyte (2009)
  • Author(s): Nishi K, Inoue H, Schnier JB, Rice RH
  • Journal Title: J Cell Biochem 106 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Cyclin Dl downregulation is important for permanent cell cycle exit and initiation of differentiation induced by anchorage-deprivation in human keratinocyte. (2009)
  • Author(s): Nishi K, Inoue H, Schnier JB, Rice RH
  • Journal Title: J Cell Biochem 106 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Iive imaging by time-laps microscopy can more clearly evaluate the anti-apoptotic state of primary hepatocytes isolated from the Drs knockout mouse (2008)
  • Author(s): Fukumoto K, Wang B, Hikosaka K, Noritake H, Shibata K, Inoue H, SuzukiA, Inaba K, Sakaguchi T, Suzuki S, KonnoH, Miura N.
  • Journal Title: Akita J Med 35 Pages: 231-237
  • Peer Reviewed
• [Journal Article] Role of alternativesplicing of periostin in human bladder carcinogenesis (2008)
  • Author(s): Kim CJ, Isono T, Tambe Y, Chano T, Okabe H, Okada Y, Inoue H
  • Journal Title: Int J Oncol 32 Pages: 161-169
  • Peer Reviewed
• [Journal Article] Role of alternative splicing of periost in in human bladder carcinogenesis (2008)
  • Author(s): Kim CJ, Isono T, Tambe Y, Chano T, Okabe H, Okada Y, Inoue H.
  • Journal Title: Int J Oncol 32 Pages: 161-169
  • Peer Reviewed
• [Journal Article] GADD34 inhibitsmammalian target of rapamycinsignaling survival under bioenergeticstress (2007)
  • Author(s): Watanabe R, Tambe Y, Inoue H, Isono T,Haneda M, Isobe K, Kobayashi T, Hino O,Okabe H, Chano T
  • Journal Title: Int J Mol Med 19 Pages: 475-483
  • Peer Reviewed
• [Journal Article] Down-regulation of drs mRNA isassociated with the progression of adult T-cell leukemia/lymphoma (2007)
  • Author(s): Shimakage M, Inoue N, Oshima K,Kawahara K, Yamamoto N, Oka T, Tambe Y,Yasui K, Matsumoto K, Yutsudo M, Inoue H
  • Journal Title: Int J Oncol 30 Pages: 1343-1348
  • Peer Reviewed
• [Journal Article] GADD34 induces p21expression and cellular senescence (2007)
  • Author(s): Minami K, Inoue H, Terashita T,Kawakami T, Watanabe R, Haneda M, IsobeK, Okabe H, Chano T
  • Journal Title: Oncol Rep 7 Pages: 1481-1485
  • Peer Reviewed
• [Journal Article] Suppression of viral replication by stress-inducible GADD34 protein via the mammalian serine/threonine protein kinase mTOR pathway (2007)
  • Author(s): Minami K, Tambe Y, Watanabe R, Isono T,Haneda M, Isobe K, Kobayashi K, Hino O, Okabe H, Chano T, Inoue H
  • Journal Title: J Virol 81 Pages: 11106-11115
  • Peer Reviewed
• [Journal Article] Tumor prone phenotype of mice deficient in a novel apoptosis-induced gene (2007)
  • Author(s): Tambe Y, Yoshioka-Yamashita A, Mukaisho K, Haraguchi S, Chano T, Isono T, Kawai T, Suzuki Y, Kushima R, Tattori T, Goto M, Yamada S, Kiso M, Saga Y, Inoue H
  • Journal Title: drs. Carcinogenesis 28 Pages: 777-784
  • Peer Reviewed
• [Journal Article] A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma (2007)
  • Author(s): Kageyama S, Iwaki H, Inoue H, Isono T, Yuasa T, Nogawa M, Maekawa T, Ueda M,Kajita Y, Ogawa O, Toguchida J, Yoshiki T
  • Journal Title: Proteimics Clin Appl 1 Pages: 192-199
  • Peer Reviewed
• [Journal Article] Up-regulation of urinary UP III mRNA levels in vesicoureteral reflux patients : potential application as a screening test for vesicoureteral reflux (2007)
  • Author(s): Iwaki H, Johnin K, Kageyama S, Kim CJ, Isono T, Yoshiki T
  • Journal Title: Int Jurol 14 Pages: 918-923
  • NAID: 10019947823
  • Peer Reviewed
• [Journal Article] Up-regulation of urinary UP III mRNA levels in vesicoureteral reflux patients: potential application as a screening test for vesicoureteral reflux (2007)
  • Author(s): Iwaki H, Johnin K, Kageyama S, Kim CJ, Isono T, Yoshiki T.
  • Journal Title: Int J Urol 14 Pages: 918-923
  • NAID: 10019947823
  • Peer Reviewed
• [Journal Article] A novel tumor-related protein,C70rf24,identified by proteome differential display of bladder urothelial carcinoma (2007)
  • Author(s): Kageyama S, Iwaki H, Inoue H, Isono T, Yuasa T, Nogawa M, Maekawa T, Ueda M, Kajita Y, Ogawa O, Toguchida J, Yoshiki T.
  • Journal Title: Proteomics Clin Appl 1 Pages: 192-199
  • Peer Reviewed
• [Journal Article] Tumor prone phenotype of mice deficient in a novel apoptosis'induced gene,drs (2007)
  • Author(s): Tambe Y, Yosbioka-Yamashita A, Mukaisho K, Haraguchi S, Chano T, Isono T, Kawai T, Suzuki Y, Kushima R, Hattori T, Goto M, Yamada S, Kiso M, Saga Y, Inoue H.
  • Journal Title: Carcinogenesis 28 Pages: 777-784
  • Peer Reviewed
• [Journal Article] Suppression of viral replication by stress-inducible GADD34 protein via the mammalian serine/threonione protein kinase mTOR pathway (2007)
  • Author(s): Minami K, Tambe Y, Watanabe R, Isono T, Haneda M, Isobe K, Kobayashi K, Hino O, 0kabe H, Chano T, Inoue H.
  • Journal Title: J Virol 81 Pages: 11106-11115
  • Peer Reviewed
• [Journal Article] Evaluations for hydronephrosis of tubeless cutaneous ureterostomy using MAG3 diuretic renography
  • Author(s): Kim CJ, Takimoto K, Tomita K, Osafune T, Nishikawa N, Johnin K, Okada Y.
  • Journal Title: Clin Nucl Med (in press)
  • Peer Reviewed
• [Journal Article] Suppression of cell invasiveness by periostin via TAB1/TAK1
  • Author(s): Isono T, Kim CJ, Ando Y, Sakurai H, Okada Y, Inoue H
  • Journal Title: Int J Oncol (in press)
  • Peer Reviewed
• [Journal Article] The Drs tumor suppressor is involved in the maturation process of antophagy induced by low serum
  • Author(s): Tambe Y, Yamamoto A, Isono T, Chano T, Fukuda M, Inoue H
  • Journal Title: Cancer Lett (in press)
  • Peer Reviewed
• [Journal Article] Cyclin D1b variant promotes cell invasiveness independent of binding to CDK4 in human bladder cancer cells
  • Author(s): Kim CJ, Nishi K, Isono T, Okuyama Y, Tambe Y, Okada Y, Inoue H
  • Journal Title: Mol Carcinog (in press)
  • Peer Reviewed
• [Journal Article] Cyclin Dlb variant promotes cell invasiveness independent of binding to CDK4 in human bladder cancer cells.
  • Author(s): Kim CT, Nishi K, Isono T, Okuyama Y, Tambe Y, Okada Y, Inoue H
  • Journal Title: Mol Carcinogenesis (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Downregulation of drs tumor suppressor gene in highly malignant human pulmonary neuroendocrine tumors.
  • Author(s): Shimakage M, Kodama K, Kawahara K, Kim CJ, Ikeda Y, Yutsudo, M, Inoue H
  • Journal Title: Oncol Rep (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] The Drs tumor suppressor is involved in the maturation precess of autophagy induced by low serum
  • Author(s): Tambe Y, Yamamoto A, Isono T, Chano T, Fukuda M, Inoue H
  • Journal Title: Cancer Letters (In press)(掲載確定)
  • Peer Reviewed
• [Presentation] エネルギー枯渇に対するストレス応答における癌抑制遺伝子drsの役割 (2008)
  • Author(s): 旦部幸博, 前田理亜, 山本章嗣, 茶野徳宏, 井上寛一
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-11
• [Presentation] Splicing variant cyclin D1bによる膀胱癌細胞の悪性化促進の解析 (2008)
  • Author(s): 井上寛一, 西香代子, 奥山雄介, 旦部幸博, 岡田裕作, 礒野高敬, 金哲將
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] Periostin による癌の浸潤・転移抑制機構の解析 (2008)
  • Author(s): 礒野高敬, 金哲將, 安藤幸宏, 櫻井宏明, 山本章嗣, 井上寛一
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] Periostinによる癌の浸潤・転移抑制機構の解析 (2008)
  • Author(s): 礒野高敬, 金哲將, 安藤幸宏, 櫻井宏明, 山本章嗣, 井上寛一
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] Splicing variant cyclin Dlbによる膀胱癌細胞の悪性化促進の解析 (2008)
  • Author(s): 井上寛一, 西香代子, 奥山雄介, 旦部幸博, 岡田裕作, 礒野高敬, 金哲將
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] Periostin associates with TAB1 to activates TAK1 and suppresses cell invasiveness (2008)
  • Author(s): Isono T, Kim CJ, Sakurai H, Okada Y, Tambe Y, Inoue H
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-29
• [Presentation] Periostin associates with TAB1 to activate TAK1 and suppresses cell invasiveness (2008)
  • Author(s): Isono T, Kim CJ, Sakurai H, Okada Y, Tambe Y, Inoue H
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-29
• [Presentation] Cyclin D1b variant enhances cell invasiveness of human bladder cancer cells (2008)
  • Author(s): Kim CJ, Isono T, Tambe Y, Okada Y, Inoue H
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-28
• [Presentation] Cyclin Dlb variant enhances cell invasiveness of human bladder cancer cells (2008)
  • Author(s): Kim CJ, Isono T, Tambe Y, Okada Y, Inoue H
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-28
• [Presentation] MAG3 利尿レノグラムによるチューブレス尿管皮膚瘻の評価 (2008)
  • Author(s): 金哲將, 長船 崇, 瀧本啓太, 西川信之, 上仁数義
  • Organizer: 第96回日本泌尿器科学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-26
• [Presentation] Stress-inducible GADD34 interacts with TSC1/2 to suppress mTOR signaling pathway (2007)
  • Author(s): Inoue H, Minami K, Tambe Y, Isono T, Chano T
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-05
• [Presentation] Analysis of oncogenic activities of cyclin D1b variant in human bladder cancer cells (2007)
  • Author(s): Kim CJ, Isono T, Tambe Y, Okada Y, Inoue H
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-05
• [Presentation] Analysis of oncogellic activities of cyclin Dlb variant in human bladder cancer cells. (2007)
  • Author(s): Kim CJ, Isono T, Tambe Y, Okada Y, Inoue H.
  • Organizer: 第66回目本癌学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-05
• [Presentation] Periostin activates TAK1 and suppresses cell invasiveness (2007)
  • Author(s): Isono T, Kim CJ, Sakurai H, Okada Y, Inoue H
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-03
• [Presentation] Periostin activates TAK1 and suppresses cell invasiveness. (2007)
  • Author(s): Isono T, Kim CJ, Sakurai H, Okada Y, Inoue H.
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-03