Elucidation of the pathogenesis of avascularity in the macula viavascular- and molecular -biological approach

• Project/Area Number: 19592032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nagoya City University
• Principal Investigator: NOZAKI Miho Nagoya City University, 大学院・医学研究科, 講師 (00295601)
• Co-Investigator(Kenkyū-buntansha): OGURA Yuichiro 名古屋市立大学, 大学院・医学研究科, 教授 (70191963) ; SAKURAI Eiji 名古屋市立大学, 大学院・医学研究科, 講師 (30305528) ; YASUKAWA Tsutomu 名古屋市立大学, 大学院・医学研究科, 准教授 (00324632)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 加齢黄斑変性 / 脈絡膜新生血管 / VEGF-A / SPARC / レーザー / 実験的脈絡膜新生血管 / レクチン / CCR3

Research Abstract

なぜ加齢黄斑変性は黄斑部にのみ脈絡膜新生血管が発生するのか?なぜ黄斑部(中心窩)は無血管なのか?血管新生内皮増殖因子(VEGF-A)を血管新生促進あるいは抑制に働かせるスイッチの働きを持つSPARC に着目し、マウスレーザー誘導実験的脈絡膜新生血管モデルを用いて検討した。レーザー照射後、SPARCの投与により、血管新生は有意に促進され、黄斑部における血管新生促進作用にSPARCが関与しており、今後加齢黄斑変性の治療ターゲットになりうると考えられた

Research Products

• [Journal Article] Sequence- and target-independent (2008)
  • Author(s): Kleinman ME, Yamada K, Takeda A, Chandrasekaran V, Nozaki M, Baffi JZ, Albuquerque RJ, Yamasaki S, Itaya M, Pan Y, Appukuttan B, Gibbs D, Yang Z, Kariko K, Ambati BK, Wilgus TA, DiPietro LA, Sakurai E, Zhang K, Smith JR, Taylor EW, Ambati J
  • Journal Title: angiogenesis suppression by siRNA via TLR3 452(7187) Pages: 591-597
  • Peer Reviewed
• [Journal Article] Toward a higher fidelity model of AMD (2008)
  • Author(s): Raisler BJ, Nozaki M, Baffi J, Hauswirth WW, Ambati J
  • Journal Title: Adv Exp Med Biol 613 Pages: 185-192
• [Journal Article] Sequence- and target-independent angiogenesis suppression by siRNA via TLR3. (2008)
  • Author(s): Kleinman ME, Yamada K, Takeda A, Chandrasekaran V, Nozaki M, et al
  • Journal Title: Nature 452 Pages: 591-597
  • Peer Reviewed
• [Journal Article] トリアムシノロン局所投与後の後嚢下白内障の発症頻度. (2008)
  • Author(s): 吉村将典, 平野佳男, 野崎実穂, 吉田宗徳, 小椋祐一郎
  • Journal Title: 日眼会誌 112 Pages: 786-789
  • NAID: 10024370050
  • Peer Reviewed
• [Journal Article] Toward a higher fidelity model of AMD. (2008)
  • Author(s): Raisler BJ
  • Journal Title: Adv Exp Med Biol 613 Pages: 185-192
• [Journal Article] Upregulation of VEGF in murine retina via monocyte recruitment after retinal scatter laser photocoagulation (2007)
  • Author(s): Itaya M, Sakurai E, Nozaki M, Yamada K, Yamasaki S, Asai K, Ogura Y
  • Journal Title: Invest Ophthalmol Vis Sci 48(12) Pages: 5677-5683
  • Peer Reviewed
• [Journal Article] A case of adult limbal xanthogranuloma (2007)
  • Author(s): Hirata M, Nozaki M, Ozeki H, Hirata F, Ogura Y
  • Journal Title: Jpn J Ophthalmol 51(4) Pages: 302-304
  • NAID: 10019830257
  • Peer Reviewed
• [Journal Article] Upregulation of VEGF in murine retina via monocyte recruitment after retinal scatter laser photocoagulation (2007)
  • Author(s): Itaya M
  • Journal Title: Invest Ophthalmol Vis Sci 48 Pages: 5677-5683
  • Peer Reviewed
• [Presentation] Non-targeted siRNA suppresses laser-induced choroidal neovascularization (2009)
  • Author(s): 野崎実穂
  • Organizer: 第34回日本微小循環学会
  • Place of Presentation: 東京、日本
  • Year and Date: 2009-02-21
• [Presentation] Non-targeted siRNA suppresses laser-induced choroidal neovascul arization. (2009)
  • Author(s): 野崎実穂, 芦苅正幸, 他
  • Organizer: 第34回日本微小循環学会
  • Place of Presentation: 東京
  • Year and Date: 2009-02-21
• [Presentation] Long-Term Results of Surgical Removal of Choroidal Neovascularization- Series of a Single Surgeon in 10 Years (2008)
  • Author(s): M. Nozaki
  • Organizer: WOC
  • Place of Presentation: 香港、中国
• [Presentation] Targeted and Non-Targeted Small Interfering RNAs Equally Suppress Choroidal Neovascularization Through (2008)
  • Author(s): M. Nozaki
  • Organizer: Activation of Toll-Like Receptor
  • Place of Presentation: ウィーン、オーストリア
• [Presentation] CCR3阻害による脈絡膜新生血管抑制 (2007)
  • Author(s): 野崎実穂
  • Organizer: 日本眼循環学会
  • Place of Presentation: 香川、日本
• [Presentation] Topical gene ablation of sflt-1 abolishes corneal avascularity (2007)
  • Author(s): R. Albuquerque , M. Nozaki, et.al.
  • Organizer: ARVO
  • Place of Presentation: フォートローダーデール、米国
• [Presentation] Topical Gene Ablation of sflt-1 Abolishes Corneal Avascularity (2007)
  • Author(s): R. Albuquerque
  • Organizer: ARVO
  • Place of Presentation: Fort Lauderdale, USA