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Global gene expression in periodontopathogeic bacterium biofilm

Research Project

Project/Area Number 19592127
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

KAMAGUCHI Arihide  Health Sciences University of Hokkaido, 歯学部, 准教授 (40133235)

Co-Investigator(Kenkyū-buntansha) NAKAZAWA Futoshi  北海道医療大学, 歯学部, 教授 (60115053)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
KeywordsPorphyromonas gingivalis / バイオフィルム / 浮遊菌 / DNAアレイ / クオーラムセンシング / AI-2 / 菌の遊離 / Streptococcus gordonii / 口腔細菌 / 歯周病 / 歯周病原因菌 / タンパク質 / Porphytomonas gingivalis / 遺伝子 / 遺伝子発現
Research Abstract

Gene expression analysis between biofilm cells and planktonic cells of Porphyromonas gingivalis which is one of the major aetiological agents in advance adult periodontitis was done with DNA microarray. Upregulated genes were gene for uptake of nutrient and secretion of signal molecules, gene for energy metabolism, gene for two component system and so on. Downregulated genes were gene for heat shock protein, gene for transcription under oxidative stress, phosphatase and so on. In this study, it was shown that difference of gene expression occurred between biofilm cells and plaktonic cells of P. gingivalis. Besides, it implies that that ABC trasporters are associated to formation of P. gingivalis biofilm.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (24 results)

All 2010 2009 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (18 results)

  • [Journal Article] Identivication of oral species of the genus Veillonella by polymerase chain reaction2009

    • Author(s)
      Igarashi E, Kamaguchi A, Fujita M, Miyakawa H, Nakazawa F.
    • Journal Title

      Oral Microbiology Immunology 24

      Pages: 310-313

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of oral speceies of the genus Veillonella by polymerase chain reaction2009

    • Author(s)
      Igarashi E., et al.
    • Journal Title

      Oral Microbiology Immunology 24

      Pages: 310-313

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro evaluation of the antimicrobial activity of a new resinbased endodontic sealer against endodontic pathogens.2008

    • Author(s)
      Yasuda Y. Kamaguchi A. Saito T.
    • Journal Title

      Journal of Oral Science 50

      Pages: 309-313

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis無色突然変異株のバイオフィルム形成性2008

    • Author(s)
      鎌口有秀、岡本公彰、五十嵐英次、藤田真理、宮川博史、中澤太
    • Journal Title

      北医療大歯誌 27

      Pages: 85-92

    • NAID

      120005429007

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis無色突然変異株のバイオフィルム形成性2008

    • Author(s)
      鎌口有秀, 他
    • Journal Title

      北海道医療大学歯学雑誌 27

      Pages: 85-92

    • NAID

      120005429007

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of recombinant hemagglutinin derived from the gingipain-encoding gene of Porphyromonas gingivalis, identification of its target protein on erythrocytes, and inhibition of hemagglutination by an interdomain regional peptide2007

    • Author(s)
      Sakai E, Naito M, Sato K, Hotokezaka H, Kadowaki T, Kamaguchi A, Yamamoto K, Nakayama K.
    • Journal Title

      Journal of Bacteriology 189

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] Streptococcus gordoniiのバイオフィルムに対するPorphyromonas gingivalis培養上清の影響2010

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第83回日本細菌学会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] コーヒーのPorphyromonas gingivalisのシステインプロテアーゼの阻害効果2010

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第28回北海道医療大学歯学会
    • Place of Presentation
      札幌
    • Year and Date
      2010-03-06
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Streptococcus gordonii菌体に対するPorphyromonas gingivalis培養上清の影響2009

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第51回歯科基礎医学会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Porphyromonas gingivalisのバイオフィルムについての検討2009

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第81回日本細菌学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-12
    • Related Report
      2009 Final Research Report
  • [Presentation] Porphyromonas gingivalisのrelA/spoT変異株の性状とバイオフィルム形成性2009

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第27回北海道医療大学歯学会
    • Place of Presentation
      札幌
    • Year and Date
      2009-02-28
    • Related Report
      2009 Final Research Report
  • [Presentation] Porphyromonas gingivalisのバイオフィルムについての検討2009

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第82回日本細菌学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] バイオフィルムへのPorphyromonas gingivalisの参入とその後の動向の検討の為の基礎的研究2008

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第50回歯科基礎医学会
    • Place of Presentation
      東京
    • Year and Date
      2008-09-23
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Porphyromonas gingivalisのバイオフィルム形成菌と浮遊菌の遺伝子発現について2008

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第76回日本細菌学会北海道支部会
    • Place of Presentation
      札幌
    • Year and Date
      2008-09-06
    • Related Report
      2009 Final Research Report
  • [Presentation] Gene expression of Porphyromonas gingivalis biofilm.2008

    • Author(s)
      Kamaguchi
    • Organizer
      86th General session and exhibitionof IADR
    • Place of Presentation
      Toront
    • Year and Date
      2008-06-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Porphyromonas gingivalis親株とluxS変異株のバイオフィルム形成性2008

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第81回日本細菌学会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-26
    • Related Report
      2009 Final Research Report
  • [Presentation] Porphyromonas gingivalis親株とluxS変異株のバイオフィルム形成性2008

    • Author(s)
      鎌口 有秀
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都(国立京都国際会館)
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] 、Porphyromonas gingivalisの浮遊菌とバイオフィルム形成菌の遺伝子発現の比較2008

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第26回北海道医療大学歯学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-01
    • Related Report
      2009 Final Research Report
  • [Presentation] Porphyromonas gingivalisの浮遊菌とバイオフィルム形成菌の遺伝子発現の比較2008

    • Author(s)
      鎌口 有秀
    • Organizer
      第26回北海道医療大学歯学会学術大会
    • Place of Presentation
      札幌(北海道医療大学札幌サテライトキャンパス)
    • Year and Date
      2008-03-01
    • Related Report
      2007 Annual Research Report
  • [Presentation] Gene expression of Porphyromonas gingivalis biofilm2008

    • Author(s)
      Kamaguchi A., et al.
    • Organizer
      86^<th> General Session and Exhibition of the IADR
    • Place of Presentation
      トロント(カナダ)
    • Related Report
      2008 Annual Research Report
  • [Presentation] Porphyromonas gingivalisのバイオフィルム形成に対するQuorum sensingの影響2007

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第75回日本細菌学会北海道支部会
    • Place of Presentation
      札幌
    • Year and Date
      2007-09-08
    • Related Report
      2009 Final Research Report
  • [Presentation] Porphyromonas gingivalisのバイオフォルムに対するQuorum sensingの影響2007

    • Author(s)
      鎌口 有秀
    • Organizer
      第75回日本細菌学会北海道支部学術総会
    • Place of Presentation
      札幌(北海道医療大学札幌サテライトキャンパス)
    • Year and Date
      2007-09-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Porphyromonas gingivalisのrelA/spoT変異株の発育とバイオフィルム形成性2007

    • Author(s)
      鎌口有秀, 他
    • Organizer
      第49回歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-31
    • Related Report
      2009 Final Research Report
  • [Presentation] Porphyromonas gingivalisのrelA/spoT変異株の発育とバイオフィルム形成性2007

    • Author(s)
      鎌口 有秀
    • Organizer
      第49回歯科基礎医学会総会
    • Place of Presentation
      札幌(北海道大学学術交流会館)
    • Year and Date
      2007-08-31
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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