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Identification of phosphorylated proteins in murine B cells in relation to the LPS-induced proliferative response

Research Project

Project/Area Number 19592129
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionIwate Medical University

Principal Investigator

KIMURA Shigenobu  Iwate Medical University, 歯学部, 教授 (10177917)

Co-Investigator(Kenkyū-buntansha) OHARA-NEMOTO Yuko  長崎大学大学院, 医歯薬学総合研究科, 准教授 (10164667)
佐々木 実  岩手医科大学, 歯学部, 准教授 (40187133)
田近 志保子  岩手医科大学, 歯学部, 講師 (20048531)
Co-Investigator(Renkei-kenkyūsha) SASAKI Minoru  岩手医科大学, 歯学部, 准教授 (40187133)
TAJIKA Shihoko  岩手医科大学, 歯学部, 講師 (20048531)
SHIMOYAMA Yu  岩手医科大学, 歯学部, 助教 (90453331)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
KeywordsLPS / B細胞 / 細胞内リン酸化反応 / 細胞内チロシンリン酸化反応
Research Abstract

LPSによるB細胞の増殖活性にかかわる細胞内シグナル伝達系を明らかにする目的で, LPS刺激後のB細胞の増殖反応と細胞内リン酸化反応について検討を行った結果, 増殖反応がみられた場合には25kDaの基質タンパク質(p25)のチロシンリン酸化反応が誘導された. そこで, p25の性状解析を行った結果, p25はRanであること, また無刺激でも複数のp25のスポットが観察されることが明らかとなった. さらに, LPS刺激によりp25のチロシンリン酸化総量が増加し, 且つその最も酸性側のスポットの量が増加することが明らかとなった.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (44 results)

All 2009 2008 2007

All Journal Article (16 results) (of which Peer Reviewed: 7 results) Presentation (20 results) Book (8 results)

  • [Journal Article] Gene expression analyses of human macrophage phagocytizing sub-m titanium particles by allergy DNA chip (GenopalTM)2009

    • Author(s)
      Taira, M., Nezu, T., Sasaki, M., Kimura, S., Kagiya, T., Harada, H., Narushima T. and Araki, Y.
    • Journal Title

      Biomed. Mater. Eng. 19(in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Effects of fosfomycin on Shiga toxin-producing Escherichia coli : quantification of copy numbers of Shiga toxin genes and their expression levels using real-time PCR2009

    • Author(s)
      Ichinohe, N., Ohara-Nemoto, Y., Nemoto, T. K., Kimura, S. and Ichinohe, S.
    • Journal Title

      J. Med. Microbiol. (in peress)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Determination of three amino acids that caused the alteration of proteolytic activities of staphylococcal glutamyl endopeptidases2009

    • Author(s)
      Nemoto, T. K., Ono, T., Shimoyama, Y., Kimura, S. and Ohara-Nemoto, Y.
    • Journal Title

      Biol. Chem 390

      Pages: 277-285

    • Related Report
      2008 Final Research Report
  • [Journal Article] Determination of three amino acids that caused the alteration of proteolytic activities of staphylococcal glutamyl endopeptidases.2009

    • Author(s)
      Nemoto, T. K., Ono, T., Shimoyama, Y., Kimura, S., Ohara-Nemoto, Y.
    • Journal Title

      Biol. Chem. 390

      Pages: 277-285

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression analyses of human macrophage phagocytizing sub-m titanium particles by allergy DNA chip (Genopal^<TM>).2009

    • Author(s)
      Taira, M., Nezu, T., Sasaki M., Kimura, S., Kagiya, T., Harada, H., Narushima T., Araki, Y.
    • Journal Title

      Biomed. Mater. Eng. 19(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli2008

    • Author(s)
      Nemoto, T. K., Ohara-Nemoto, Y., Ono, T., Kobayakawa, T., Shimoyama, Y., Kimura, S. and Takagi, T.
    • Journal Title

      FEBS J. 275

      Pages: 573-587

    • NAID

      120006982394

    • Related Report
      2008 Final Research Report
  • [Journal Article] An Escherichia coli expression system for glutamyl endopeptidases optimized by complete suppression of auto-degradation2008

    • Author(s)
      Nemoto, T.K., Ono, T., Shimoyama, Y., Kimura, S. and Ohara-Nemoto, Y.
    • Journal Title

      Anal. Biochem 381

      Pages: 74-80

    • NAID

      120006982402

    • Related Report
      2008 Final Research Report
  • [Journal Article] Homologous and heterologous expression and maturation processing of extracellular glutamyl endopeptidase of Staphylococcus epidermidis2008

    • Author(s)
      Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T.K.
    • Journal Title

      Biol. Chem. 389

      Pages: 1209-1217

    • NAID

      120006982370

    • Related Report
      2008 Final Research Report
  • [Journal Article] TEM/EDX observation of micro-beads of magnetically activated cell sorting (MACS) system and separation trial of mouse mesenchymal stem cells from bone marrow cells by MACS system2008

    • Author(s)
      Taira, M., Sasaki, K., Saitoh, S., Nezu, T., Kagiya, T., Harada, H., Sasaki, M., Kimura, S., Hirata, I. and Araki, Y.
    • Journal Title

      J. Oral Tissue Engin. 6

      Pages: 33-40

    • NAID

      110007004837

    • Related Report
      2008 Final Research Report
  • [Journal Article] Occurrence of staphylococci in the oral cavities of healthy adults and nasal-oral trafficking of the bacteria2008

    • Author(s)
      Ohara-Nemoto, Y., Haraga, H., Kimura, S. and Nemoto, T.K.
    • Journal Title

      J. Med. Microbiol 57

      Pages: 95-99

    • NAID

      120006982334

    • Related Report
      2008 Final Research Report
  • [Journal Article] Dose-dependent effects of Ni(II)ions on production of three inflammatory cytokines(TNF-・, IL-1・ and IL-6), superoxide dismutase(SOD)and free radical NO by murine macrophage-like RAW264 cells with or without LPS-stimulation2008

    • Author(s)
      Taira, M., Sasaki,M., Kimura, S. and Araki, Y.
    • Journal Title

      J. Mater. Sci : Mater. Med. 192008.(EPub 2007, Nov 28)

      Pages: 2173-2178

    • Related Report
      2008 Final Research Report
  • [Journal Article] An Escherichia coli expression system for glutamyl end peptidases optimized by complete suppression of auto-degradation.2008

    • Author(s)
      Nemoto, T. K., Ono, T., Shimoyama, Y., Kimura. S., Ohara-Nemoto. Y
    • Journal Title

      Anal. Biochem. 381

      Pages: 74-80

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Homologous and heterologous expression and maturation processing of extreacellular glutamyl endopeptidase of Staphylococcus epidermidis.2008

    • Author(s)
      Ohara-Nemoto. Y., Ono, T., Shimoyama. Y., Kimura, S., Nemoto, T. K.
    • Journal Title

      Biol. Chem. 389

      Pages: 1209-1217

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TEMEDX observation of micro-beads of magnetically activated cell sorting (MACS) system and separation trial of mouse mesenchymal stem cells from bone marrow cells by MACS system.2008

    • Author(s)
      Taira, M., Sasaki, K, Saitoh, S., Nezu, T., Kagiya, T., Harada, H., Sasaki M., Kimura, S., Hirata, I., Araki, Y.
    • Journal Title

      J. Oral Tissue Engin. 6

      Pages: 33-40

    • NAID

      110007004837

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Occurrence of staphylococci in the oral cavities of healthy adults and nasal-oral trafficking of the bacteria.2008

    • Author(s)
      Ohara-Nemoto. Y., Haraga, H., Kimura. S.and Nemoto, T.K.
    • Journal Title

      J.Med.Microbiol. 57

      Pages: 95-99

    • NAID

      120006982334

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli.2008

    • Author(s)
      Nemoto, T.K., Ohara-Nemoto. Y., Ono, T., Kobayakawa, T., Shimoyama, Y., Kimura. S.and Takazi. T.
    • Journal Title

      FEBS J. 275

      Pages: 573-587

    • NAID

      120006982394

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Rapid identification of HACEK group bacteria using 16S rRNA gene PCR-RFLP2009

    • Author(s)
      Sasaki, M., Tajika, S., Kodama, S., Shimoyama, Y. and Kimura, S.
    • Organizer
      The 3rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai. Japan
    • Year and Date
      2009-01-16
    • Related Report
      2008 Final Research Report
  • [Presentation] A novel aspartate-specific dipeptidylpeptidase produced from Porphyromonas endodontalis2009

    • Author(s)
      Haraga, H., Ohara-Nemoto, Y., Nemoto, T. K., Shimoyama, Y., Sasaki, M. and Kimura, S.
    • Organizer
      The 3rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai. Japan
    • Year and Date
      2009-01-16
    • Related Report
      2008 Final Research Report
  • [Presentation] The production of secretory leukocyte protease inhibitor(SLPI)from gingival epithelial cells in response to Porphyromounas gingivalis lipopoly-saccharides2009

    • Author(s)
      Ishikawa, T., Ohara-Nemoto, Y., Tajika, S., Sasaki, M. and Kimura, S.
    • Organizer
      The 3rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai. Japan
    • Year and Date
      2009-01-16
    • Related Report
      2008 Final Research Report
  • [Presentation] The production of secretory leukocyte protease inhibitor (SLPI) from gingival epithelial cells in response to Porphyromounas gingivalis lipopoly saccharides.2009

    • Author(s)
      Ishikawa, T., Ohara-Nemoto, Y., Tajika, S., Sasaki, M., Kimura, S.
    • Organizer
      The 3rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2009-01-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] A novel aspartate-specific dipeptidylpeptidase produced from Porphyromonas endodontalis.2009

    • Author(s)
      Haraga, H., Ohara-Nemoto, Y., Nemoto, T. K., Shimoyama. Y., Sasaki, M., Kimura, S.
    • Organizer
      The 3rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai. Japan
    • Year and Date
      2009-01-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] Rapid identification of HACEK group bacteria using 16S rRNA gene PCR-RFLP.2009

    • Author(s)
      Sasaki, M., Tajika. S., Kodama, S., Shimoyama, Y., Kimura, S.
    • Organizer
      The 3rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai. Japan
    • Year and Date
      2009-01-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] Three amino acid substitutions are sufficient for the alteration of the activities among three members of Staphylococcal glutamic-acid-specific endopeptidase2008

    • Author(s)
      Nemoto, T. K., Ono, T., Shimoyama, Y., Kimura, S. and Ohara-Nemoto, Y.
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] Three amino acid substitutions are suffcient for the alteration of the activities among three members of Staphylococcal glutamic-acid-specific endopeptidase.2008

    • Author(s)
      Nemoto, T. K., Ono, T., Shimoyama, Y., Kimura, S., Ohara-Nemoto. Y.
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Effects of high-concentration ID10% copper ions on macrophage2008

    • Author(s)
      Taira, M., Kagiya, T., Harada, H., Sasaki, M., Kimura, S. and Araki, Y.
    • Organizer
      Annual Meeting 2008 of the Academy of Dental Materials
    • Place of Presentation
      Wurzburg, Germany
    • Year and Date
      2008-10-02
    • Related Report
      2008 Final Research Report
  • [Presentation] Effects of high-concentration ID10% copper ions on macrophage.2008

    • Author(s)
      Taira, M., Kagiya, T., Harada, H., Sasaki, M., Kimura, S., Araki, Y.
    • Organizer
      Annual Meeting 2008 of the Academy of Dental Materials.
    • Place of Presentation
      Wurzburg, Germany
    • Year and Date
      2008-10-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] Gene expression analyses of human macrophage phagocytizing sub-μtitanium particles by allergy DNA chip (Genopal^<TM>)2008

    • Author(s)
      Taira, M., Nezu, T., Sasaki, M., Kimura, S., Kagiya, T., Harada, H., Narushima, T. and Araki, Y.
    • Organizer
      International Symposium on Nanotoxi- cology Assessment and Biomedical, Environmental Application of Fine Particles and Nanotubes ISNT2008
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2008-06-16
    • Related Report
      2008 Final Research Report
  • [Presentation] Gene expression analyses of human macrophage phagocytizing sub-μutitanium particles by allergy DNA chip (Genopal^<TM>).2008

    • Author(s)
      Taira, M., Nezu, T., Sasaki, M., Kimura. S., Kagiya, T., Harada, H., Narushima, T., Araki, Y.
    • Organizer
      International Symposium on Nanotoxi-clogy Assessment and Biomedical, Environmental Application of Fine Particles and Nanotubes ISNT2008.
    • Place of Presentation
      Sapporo, Japan.
    • Year and Date
      2008-06-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] Maturation processing of glutamyl endopeptidase of Staphylococcus epidermidis2008

    • Author(s)
      Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T.K.
    • Organizer
      108th General Meeting of the American Society for Microbiology
    • Place of Presentation
      Boston, USA.
    • Year and Date
      2008-06-05
    • Related Report
      2008 Final Research Report
  • [Presentation] Maturation processing of glutamyl endopeptidase of Staphylococcus epidermidis.2008

    • Author(s)
      Ohara-Nemoto, Y., Ono, T., Shimoyama, Y. Kimura, S., Nemoto, T. K.
    • Organizer
      108th General Meeting of the American Society for Microbiology.
    • Place of Presentation
      Boston, USA.
    • Year and Date
      2008-06-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Acid tolerance of Steptococcus anginosus2008

    • Author(s)
      Sasaki, M., Tajika, S., Kodama, S. and Kimura, S.
    • Organizer
      18th European Congress of Clinical Microbiology
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2008-04-20
    • Related Report
      2008 Final Research Report
  • [Presentation] Acid tolerance of Steptococcus anginosus.2008

    • Author(s)
      Sasaki, M., Tajika, S., Kodama, S., Kimura S.
    • Organizer
      18th European Congress of Clinical Microbiology.
    • Place of Presentation
      Barcelona, Spain.
    • Year and Date
      2008-04-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] Macrophage reaction against sub-m titanium particle2007

    • Author(s)
      Taira, M., Sasaki, M., Kimura, S. and Araki, Y.
    • Organizer
      Annual Meeting of the Academy of Dental Materials 2007
    • Place of Presentation
      Fort Lauderdale, FL, USA.
    • Year and Date
      2007-10-23
    • Related Report
      2008 Final Research Report
  • [Presentation] Porphyromonas gingivalis LPS刺激によるB細胞内チロシンリン酸化反応と基質タンパク質の解析2007

    • Author(s)
      下山 佑, 佐々木 実, 田近 志保子, 原賀 裕, 石河 太知, 木村 重信
    • Organizer
      第61回日本細菌学会東北支部総会
    • Place of Presentation
      仙台
    • Year and Date
      2007-08-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] Pathogenic factors of Porphyromonas endodontalis.2007

    • Author(s)
      H.Haraga, Y.Ohara-Nemoto, T.K.Nemoto, M.Sasaki, S.Tarika, S.Hamada & S.Kimura
    • Organizer
      107th General Meeting of the American Society for Microbiology
    • Place of Presentation
      Toronto,Canada
    • Year and Date
      2007-05-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Pathogenic factors of Porphyromonas endodontalis2007

    • Author(s)
      Haraga, H., Ohara-Nemoto, Y., Nemoto, T.K., Sasaki, M., Tajika, S., Hamada, S. and Kimura, S.
    • Organizer
      107th General Meeting of the American Society for Microbiology
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2007-03-22
    • Related Report
      2008 Final Research Report
  • [Book] 基礎-I-1. 口腔臨床に必要な細菌学2008

    • Author(s)
      木村重信, 浜田茂幸
    • Publisher
      In口腔内科学(尾崎登喜雄), 飛鳥出版室, 高知
    • Related Report
      2008 Final Research Report
  • [Book] Early childhood caries and childhood periodontal diseases2007

    • Author(s)
      Kimura, S., and Ohara-Nemoto, Y.
    • Publisher
      In Pediatric Infectious Diseases Revisited(H. Schroten, & S. Wirth, eds.), Birkhauser-Verlag AG, Basel.
    • Related Report
      2008 Final Research Report
  • [Book] Proinflammatory cytokine production and leukocyte adhesion molecule expression of endothelial cells in response to Abiotrophia defectiva infection2007

    • Author(s)
      Tajika, S., Sasaki, M., Agato, S., Harada-Oikawa, R., Hamada, S. and Kimura, S.
    • Publisher
      In Interface Oral Health Science 2007(M. Watanabe, N. Takahashi, & H. Takada, eds.), Springer Japan, Tokyo.
    • Related Report
      2008 Final Research Report
  • [Book] Genome-wide gene expression analysis of human myelomonocytic cell line THP-1 exposed to lipopolysaccharide(LPS)2007

    • Author(s)
      Taira, M., Sasaki, M., Kimura, S. and Aaraki, Y.
    • Publisher
      In Interface Oral Health Science 2007(M. Watanabe, N. Takahashi, & H. Takada, eds.), Springer Japan, Tokyo.
    • Related Report
      2008 Final Research Report
  • [Book] CD14-dependent and independent B-cell activations by stimulation with lipopolysaccharide from Porphyromonas gingivalis2007

    • Author(s)
      Shimoyama, Y., Ohara-Nemoto, Y., Yamada, A., Kato, H., Tajika, S. and Kimura, S.
    • Publisher
      In Interface Oral Health Science 2007(M. Watanabe, N. Takahashi, & H. Takada, eds.), Springer Japan, Tokyo.
    • Related Report
      2008 Final Research Report
  • [Book] Early childhood caries and childhood periodontal diseases.In Pediatric Infectious Diseases Revisited(H.Schroten, &S.Wirth, eds.)2007

    • Author(s)
      Kimura. S., and Ohara-Nemoto. Y.
    • Publisher
      Birkhauser-Verlag AG,Basel.
    • Related Report
      2007 Annual Research Report
  • [Book] CD14-dependent and independent B-cell activations by stimulation with lipopolysaccharide from Porphyromonas gingivallis.In Interface Oral Health Science 2007(M.Watanabe, N.Takahashi, & H.Takada, eds.)2007

    • Author(s)
      Shimoyama, Y., Ohara-Nemoto, Y., Yamada, A., Kato, H., Taiika. S.and Kimura, S.
    • Publisher
      Springer Japan,Tokyo.
    • Related Report
      2007 Annual Research Report
  • [Book] Genome-wide gene expression analysis of human myelomonocytic cell line THP-1 exposed to lipopolysaccharide(LPS).In Interface Oral Health Science 2007(M.Watanabe, N.Takahashi, & H.Takada, eds.)2007

    • Author(s)
      Taira, M., Sasaki.M., Kimura. S.and Aaraki, Y.
    • Publisher
      Springer Japan,Tokyo.
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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