Study of a novel protein secretion system of Porphyromonas gingivalis

• Project/Area Number: 19592135
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: SAIKI Keitarou The Nippon Dental University, 生命歯学部, 講師 (30297973)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口腔細菌学 / 蛋白分泌 / ジンジパイン / 歯周病

Research Abstract

A novel outer membrane protein Sov and a novel membrane protein PG27 were identified as essential factors for the secretion of gingipain protease virulence factors of Porphyromonas gingivalis. The C-terminal portion of Sov is exposed to the extracellular milieu and involved in the modulation of Sov function. These results suggest that gingipains are secreted via a novel protein secretion pathway, and that Sov and PG27 are promising targets for antiperiodontitis drug development.

Research Products

• [Journal Article] The role of Sov protein in the secretion of gingipain protease virulence factors of Porphyromonas gingivalis. (2010)
  • Author(s): Keitarou Saiki, Kiyoshi Konishi
  • Journal Title: FEMS Micobiol Lett 302 Pages: 166-174
  • Peer Reviewed
• [Journal Article] The role of Sov protein in the secretion of gingipain protease virulence factors of Porphyromonas gingivalis. (2010)
  • Author(s): Keitarou Saiki
  • Journal Title: FEMS Microbiol.Lett. 302 Pages: 166-174
  • Peer Reviewed
• [Journal Article] PG27 is a novel membrane protein essential for a Porphyromonas gingivalis protease secretion system. (2009)
  • Author(s): Ikumi Ishiguro, Keitarou Saiki, Kiyoshi Konishi
  • Journal Title: FEMS Microbiol Lett 292 Pages: 261-267
  • Peer Reviewed
• [Journal Article] PG27 is a novel membrane protein essential for a Porphyromonas gingivalis protease secretion system. (2009)
  • Author(s): Ikumi Ishiguro
  • Journal Title: FEMS Microbiol. Lett. 292 Pages: 261-267
  • Peer Reviewed
• [Presentation] 歯周病原因菌の病原因子分泌におけるSov蛋白の役割 (2010)
  • Author(s): 才木桂太郎, 古西清司
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-27
• [Presentation] 細菌の新規蛋白質分泌系に関わる新規外膜蛋白質Sovの解析 (2009)
  • Author(s): 才木桂太郎, 古西清司
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 新規の蛋白質分泌系に関わる巨大蛋白Sovの機能必須領域の検索 (2009)
  • Author(s): 才木桂太郎, 古西清司
  • Place of Presentation: 名古屋
  • Year and Date: 2009-10-22
• [Presentation] 細菌の新規蛋白質分泌系に関わる新規外膜蛋白質Sovの解析 (2009)
  • Author(s): 才木桂太郎
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Porphyromonas gingivalisの新規蛋白分泌系に関与する蛋白質PG27の同定 (2009)
  • Author(s): 石黒生美, 才木桂太郎, 古西清司
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] 新規の蛋白質分泌系に関わる巨大蛋白Sovの機能必須領域の検索 (2009)
  • Author(s): 才木 桂太郎
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] 歯周病原因菌で見つかった新規蛋白分泌系に関わる巨大蛋白Sovの同定 (2008)
  • Author(s): 才木桂太郎, 古西清司
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-26
• [Presentation] 歯周病原因菌で見つかった新規蛋白分泌装系に関わる巨大蛋白Sovの同定 (2008)
  • Author(s): 才木桂太郎
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-26