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Histological analysis of stress reaction of the stem/progenitor cell and those niche in periodontal ligament

Research Project

Project/Area Number 19592138
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionTsurumi University

Principal Investigator

SHIMADA Akemi  Tsurumi University, 歯学部, 助教 (00339813)

Co-Investigator(Kenkyū-buntansha) SHIBATA Tatsuya  鶴見大学, 歯学部, 助教 (90323708)
NIFUJI Akira  鶴見大学, 歯学部, 教授 (00240747)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords歯根膜 / 組織幹細胞 / BrdU / label-retaining cell / 再植
Research Abstract

It is thought that the tissue stem cells slowly proliferate in the normal condition, resulting that those retain DNA label for longer time than developed cells. We used BrdU to identify the label retaining cells (LRCs) as the tissue stem cells. In the mouse periodontal ligament, BrdU-LRCs distributed around blood vessels and near cementum. The BrdU-LRCs started to proliferate one day after replantation of the molar tooth, showing that the BrdU-LRCs possibly function to regenerate the periodontal tissue. It was also found that the collagen gel culture is useful to expand the BrdU-LRCs.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (39 results)

All 2010 2009 2008 2007 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (22 results) Remarks (1 results)

  • [Journal Article] Nemo-like kinase (NLK) expression in osteoblastic cells and suppression of osteoblastic differentiation2010

    • Author(s)
      Nifuji A, Ideno H, Ohyama Y, Takanabe R, Araki R, Abe M, Noda M, Shibuya H
    • Journal Title

      Exp Cell Res Vol.316

      Pages: 1127-1136

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein related to DAN and cerberus (PRDC) inhibits osteoblastic differentiation and its suppression promotes osteogenesis in vitro2009

    • Author(s)
      Ideno H, Takanabe R, Shimada A, Imaizumi K, Araki R, Abe M, Nifuji A
    • Journal Title

      Exp Cell Res Vol.315

      Pages: 474-484

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein related to DAN and cerberus(PRDC)inhibits osteoblastic differentiation and its suppression promotes osteogenesis in vitro.2009

    • Author(s)
      Ideno H, Takanabe R, Shimada A, Imaizumi K, Araki R, Abe M, Nifuji A.
    • Journal Title

      Exp Cell Res 315

      Pages: 474-484

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein related to DAN and Cerberus(PRDC)inhibits osteoblastic differentiation and its suppression promotes osteogenesis in vitro.2009

    • Author(s)
      Ideno H, Takanabe R, Shimada A, Imaizumi K, Araki R, Abe M, Nifuji A
    • Journal Title

      Exp Cell Res 315

      Pages: 474-484

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved methods for immunohistochemical detection of BrdU in hard tissue2008

    • Author(s)
      Shimada A, Shibata T, Komatsu K, Nifuji A
    • Journal Title

      J Immunol Methods Vol.339

      Pages: 11-16

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term effects of local pretreatment with alendronate on healing of replanted rat teeth2008

    • Author(s)
      Komatsu K, Shimada A, Shibata T, Shimoda S, Oida S, Kawasaki K, Chiba M
    • Journal Title

      J Periodont Res Vol.43

      Pages: 194-200

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of Noncollagenous Components by Neutrophil Elastase Reduces the Mechanical Strength of Rat2008

    • Author(s)
      Ujiie Y, Shimada A, Komatsu K, Gomi K, Oida S, Arai T, Fukae M
    • Journal Title

      J Periodont Res Vol.43

      Pages: 22-31

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improved methods for immunohistochemical detection of BrdU in hard tissue.2008

    • Author(s)
      Shimada A, Shibata T, Komatsu K, Nifuji A
    • Journal Title

      J Immunol Methods 339

      Pages: 11-16

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of Noncollagenous Components by Neutrophil Elastase Reduces the Mechanical Strength of Rat Periodontal Ligament.2008

    • Author(s)
      Ujiie Y, Shimada A, Komatsu K, Gomi K, Oida S, Arai T, Fukae M
    • Journal Title

      J Periodont Res 43

      Pages: 22-31

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term effects of local pretreatment with alendronate on healing of replanted rat teeth.2008

    • Author(s)
      Komatsu K, Shimada A, Shibata T, Shimoda S, Oida S, Kawasaki K, Nifuji A
    • Journal Title

      J Periodont Res 43

      Pages: 194-200

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of Noncollagenous Components by Neutrophil Elastase Reduces the Mechanical Strength of Rat2008

    • Author(s)
      Ujiie Y, Shimada A, Komatsu K, Gomi K, Oida S, Arai T, Fukae M
    • Journal Title

      J Periodont Res 43

      Pages: 22-31

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of contribution of collagen fibre component in viscoelastic behaviour of periodontal ligament using enzyme probe2008

    • Author(s)
      Komatsu K, Shibata T, Shimada A
    • Journal Title

      J Biomech 40(印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term effects of local pretreatment with alendronate on healing of replanted rat teeth2008

    • Author(s)
      Komatsu K, Shimada A, Shibata T, Shimoda S, Oida S, Kawasaki K, Chiba M
    • Journal Title

      J Periodont Res 43(印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of contribution of collagen fibre component in viscoelastic behaviour of periodontal ligament using enzyme probe2007

    • Author(s)
      Komatsu K, Shibata T, Shimada A
    • Journal Title

      J Biomech Vol.40

      Pages: 2700-2706

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stress-relaxation and microscopic dynamics of rabbit periodontal ligament2007

    • Author(s)
      Komatsu K, Sanctuary C, Shibata T, Shimada A, Botsis J
    • Journal Title

      J Biomech Vol.40

      Pages: 634-644

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stress-relaxation and microscopic dynamics of rabbit periodontal ligament2007

    • Author(s)
      Komatsu K, Sanctuary C, Shibata T, Shimada A, Botsis J
    • Journal Title

      J Biomech 40

      Pages: 634-644

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 骨再生を目的としたプラスミドDNAによるin vivo遺伝子導入のためのキャリアーの検討2009

    • Author(s)
      柴田達也、島田明美、出野尚、田畑泰彦、二藤彰
    • Organizer
      第27回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2009-07-25
    • Related Report
      2009 Final Research Report
  • [Presentation] 骨芽細胞の生存と分化に及ぼすアレンドロネートの影響は分化段階により異なる2009

    • Author(s)
      小松浩一郎、島田明美、柴田達也、出野尚、二藤彰
    • Organizer
      第27回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2009-07-25
    • Related Report
      2009 Final Research Report
  • [Presentation] 骨芽細胞の生存と分化に及ぼすアレンドロネートの影響は分化段階により異なる2009

    • Author(s)
      小松浩一郎、島田明美、柴田達也、出野尚、二藤彰
    • Organizer
      第27回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2009-07-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 骨再生を目的としたプラスミドDNAによるin vivo遺伝子導入のためのキャリアーの検討2009

    • Author(s)
      柴田達也、島田明美、出野尚、田畑泰彦、二藤彰
    • Organizer
      第27回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2009-07-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生体吸収性材料とBMP-2プラスミドDNAの組合せによる頭蓋骨欠損修復の試み2009

    • Author(s)
      柴田達也、島田明美、出野尚、田畑泰彦、二藤彰
    • Organizer
      第8回日本再生医療学会総会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2009-03-05
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Alendronate affects proliferation and alkaline phosphatase activity of osteoblasts2009

    • Author(s)
      Komatsu K, Shimada A, Shibata T, Ideno H, Nifuji A
    • Organizer
      2nd Meeting of IADR Pan Asian Pacific Federation (PAPF) and the 1st Meeting of IADR Asia/Pacific Region (APR)
    • Place of Presentation
      Shangri-La Hotel, Wuhan, China
    • Related Report
      2009 Final Research Report
  • [Presentation] Alendronate Affects Proliferation and Alkaline Phosphatase Activity of Osteoblasts2009

    • Author(s)
      K.KOMATSU, A.SHIMADA, T.SHIBATA, H.IDENO, A.NIFUJI
    • Organizer
      2nd Meeting of IADR Pan Asian Pacific Federation (PAPF) and the 1st Meeting of IADR Asia/Pacific Region (APR)
    • Place of Presentation
      Shangri-La Hotel, Wuhan, China
    • Related Report
      2009 Annual Research Report
  • [Presentation] 頭蓋骨欠損モデルを用いた安全な骨再生法の探究2008

    • Author(s)
      柴田達也, 島田明美, 出野尚, 荒木香映子, 石川万里子, 只木麻友, 田畑泰彦, 二藤彰
    • Organizer
      鶴見大学歯学会第68回例会
    • Place of Presentation
      鶴見大学会館メインホール
    • Year and Date
      2008-12-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 局所適用アレンドロネートの骨リモデリングに及ぼす影響2008

    • Author(s)
      小松浩一郎、島田明美、柴田達也、二藤彰
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2009 Final Research Report
  • [Presentation] プラスミドDNA遺伝子導入による骨再生の試み2008

    • Author(s)
      柴田達也、島田明美、出野尚、田畑泰彦、二藤彰
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 硬組織におけるBrdU免疫組織化学の高感度化2008

    • Author(s)
      島田明美、柴田達也、小松浩一郎、二藤彰
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 硬組織におけるBrdU免疫組織化学の高感度化2008

    • Author(s)
      島田 明美, 柴田 達也, 小松 浩一郎, 二藤 彰
    • Organizer
      第26回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] プラスミドDNA遺伝子導入による骨再生の試み2008

    • Author(s)
      柴田達也, 島田明美, 出野尚, 田畑泰彦, 二藤彰
    • Organizer
      第26回日木骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 局所適用アレンドロネートの骨リモデリングに及ぼす影響2008

    • Author(s)
      小松浩一郎, 島田明美, 柴田達也, 二藤彰
    • Organizer
      第26回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] Effect of topical alendronate on bone formation around replanted teeth2008

    • Author(s)
      Komatsu K, Shibata T, Shimada A, Nifuji A
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Pacifico Yokohama Conference Center
    • Year and Date
      2008-03-17
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] 骨再生を目指したプラスミドDNAによる頭蓋骨欠損修復モデルの確立2008

    • Author(s)
      荒木香映子、石川万里子、只木麻友、柴田達也、島田明美、出野尚、田畑泰彦、二藤彰
    • Organizer
      第21回日本歯科医学会総会
    • Place of Presentation
      パシフィコ横浜会議センター
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] アレンドロネート局所適用した再植歯周囲の骨形成2007

    • Author(s)
      柴田達也、小松浩一郎、島田明美、二藤彰
    • Organizer
      文部科学省ハイテク・リサーチ・センター整備事業(平成17〜19年度)顎口腔機能研究プロジェクト中間報告公開シンポジウム
    • Place of Presentation
      鶴見大学会館メインホール
    • Year and Date
      2007-11-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラット歯根膜の機械的性質における非コラーゲン性タンパク質の役割2007

    • Author(s)
      島田明美, 小松浩一郎, 氏家優子, 五味一博, 二藤彰, 深江允
    • Organizer
      日本歯周病学会50周年記念大会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2007-09-22
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] 硬組織におけるBrdU免疫組織化学の最適化2007

    • Author(s)
      島田明美、柴田達也、小松浩一郎、二藤彰
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2007-08-31
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] アレンドロネート局所適用した再植歯周囲の骨密度と細胞増殖2007

    • Author(s)
      小松浩一郎、柴田達也、島田明美、二藤彰
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2007-08-30
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] Protein Related to DAN and Cerberus (PRDC) is expressed in skeletogenesis and prevents osteoblastic differentiation2007

    • Author(s)
      Nifuji A, Ideno H, Takanabe R, Shimada A, Araki R, Abe M
    • Organizer
      The 29th meeting of the American Society for Bone and Mineral Research (ASBMR)
    • Place of Presentation
      Honolulu, Hawaii
    • Related Report
      2009 Final Research Report
  • [Presentation] Protein Related to DAN and Cerberus(PRDC)is expressed in skeletogenesis and prevents osteoblastic differentiation2007

    • Author(s)
      Nifuji A, Ideno H, Takanabe R, Shimada A, Araki R, Abe M
    • Organizer
      The 29th meeting of the American Society for Bone and Mineral Research(ASBMR)
    • Place of Presentation
      Honolulu, Hawaii
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://ccs.tsurumi-u.ac.jp/dental/kouza/yakuri.htm

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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