Regulation of CCN family gene expression via micro RNA regulatory network and its Biological signficance
| Project/Area Number |
19592142
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
KUBOTA Satoshi Okayama University, 大学院・医歯薬学総合研究科, 准教授 (90221936)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIGAWA Masaharu 岡山大学, 大学院・医歯薬学総合研究科, 教授 (20112063)
HATTORI Takako 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00228488)
NISHIDA Takashi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (30322233)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 口腔生化学 / ゲノム医化学 / 細胞医化学 / CCN2 / miRNA / 軟骨 / miR-18 / CCN family / 結合組織成長因子 |
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| Research Abstract |
マイクロRNA(miRNA)は小分子noncoding RNA(ncRNA)であり、個々のmiRNAが数千のmRNAの3'非翻訳領域を標的として、遺伝子の発現をネットワーク的に制御する。本研究ではCCNファミリー遺伝子の代表的メンバーであり、間葉系組織の成長ならびに再生を指揮するCCN2遺伝子が特定のmiRNAの制御ネットワーク下にあることを実証した。さらに、このmiRNAによる制御が、軟骨細胞の成熟形質の獲得・維持に重要であることも明らかとなった。またmiRNAの作用機構や軟骨細胞後期分化における役割を今後解明していく上で、有用な知見をも得ることができた。
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Report
(3 results)
Research Products
(107 results)
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[Journal Article] Cooperative regulation of chondrocyte differentiation by CCN2 and CCN3 revealed by a comprehensive analysis of the CCN family proteins in cartilage2008
Author(s)
Kawaki, T., S.Kubota, A.Suzuki, N.Lazar, T.Yamada, T.Matsumura, T.Ohgawara, T.Maeda, B.Perbal, K.M.Lyons, and M.Takigawa
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Journal Title
J. Bone Miner. Res 23
Pages: 1751-1764
Related Report
Peer Reviewed
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[Journal Article] Promotion of bone regeneration by CCN2 incorporated into gelatin hydrogel2008
Author(s)
Kikuchi, T., S.Kubota, K.Asaumi, H.Kawaki, K.Nishida, T., Kawata, S.Mitani, Y.Tabata, T.Ozaki, and M.Takigawa
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Journal Title
Tissue Engineering Part A 14
Pages: 1089-1098
NAID
Related Report
Peer Reviewed
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[Journal Article] Promotion of hydroxyapatite-associated, stem cell-based bone regeneration by CCN22008
Author(s)
Ono, M., S.Kubota, T.Fujisawa, W.Sonoyama, H.Kawaki, K.Akiyama, M.Oshima, T.Nishida, Y.Yoshida, K.Suzuki, M.Takigawa and T.Kuboki
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Journal Title
Cell Transplant 17
Pages: 231-240
Related Report
Peer Reviewed
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[Journal Article] Plasma connective tissue growth factor is a novel potential biomarker of cardiac dysfunction in patients with chronic heart failure2008
Author(s)
Koitabashi, N., M.Arai, K.Niwano, A.Watanabe, M.Endoh, M.Suguta, T.Yokoyama, H.Tada, T.Toyama, H.Adachi, S.Naito, S.Oshima, T.Nishida, S.Kubota, M.Takigawa and M.Kurabayashi
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Journal Title
Eur. J. Heart Failure 10
Pages: 373-379
Related Report
Peer Reviewed
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[Journal Article] Promotion of attachment of human bone marrow stromal cells by CCN2. Biochem2007
Author(s)
Ono, M., S.Kubota, T.Fujisawa, W.Sonoyama, H.Kawaki, K.Akiyama, M.Oshima, T.Nishida, Y.Yoshida, K.Suzuki, M.Takigawa and T.Kuboki
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Journal Title
Biophys. Res. Commun 357
Pages: 20-25
Related Report
Peer Reviewed
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[Journal Article] Increased connective tissue growth factor relative to brain natriuretic peptide as a determinant of myocardial fibrosis2007
Author(s)
Koitabashi, N., M.Arai, S.Kogure, K.Niwano, A.Watanabe, Y.Aoki, T.Maeno, T.Nishida, S.Kubota, M.Takigawa and M.Kurabayashi
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Journal Title
Hypertension 49
Pages: 1120-1127
Related Report
Peer Reviewed
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[Presentation] Expression and mechanisms of connective tissue growth factor (CCN2/CTGF) induction in osteolytic jaw invasion of human oral squamous cell carcinoma2009
Author(s)
Shimo, T., S.Kubota, T.Goda, Y.Yoshihama, N.Kurio, T.Okui, D.Yamamoto, A.Ono, M.Takigawa and A.Sasaki
Organizer
ORS 55th Annual Meeting
Place of Presentation
Las Vegas, U.S.A.
Related Report
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[Presentation] Roles of CCN2 in skeletal growth and regeneration -requirement for both endochondral and intramembranous bone formation2008
Author(s)
Takigawa, M., N.Tomita, T.Hattori, H.Kawaki, S.Kubota, T.Kikuchi, S.Itoh, E.Aoyama, M.Yao, A.Suzuki, T.Maeda, K.Asaumi, T.Nishida, T.Ozaki, T.Yamashiro, K.M.Lyons, Y.Tabata
Organizer
Fifth International Workshop on the CCN Family of Genes
Place of Presentation
Toronto、Canada
Related Report
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