The application of functional elucidation in epithelial-mesenchymal interaction to the practical treatment in chronic periodontitis

• Project/Area Number: 19592167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tohoku University
• Principal Investigator: SHIMONISHI Mitsuru Tohoku University, 病院, 助教 (40302153)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 免疫 / 感染 / 炎症 / マラッセの上皮遺残 / MMP-2 / 細胞間相互作用 / 免疫・感染・炎症

Research Abstract

The tissues were sampled from the root of human extracted teeth and produced outgrowths containing both epithelial cells and fibroblasts from human periodontal ligaments (HPDL). Type IV collagen were found at the interface between cells of the epithelial rests of Malassez (ERM cells) and HPDL fibroblasts. MMP-2 can degrade type IV collagen. The current study was undertaken to examine the expression of MMP-2 at the interface. These findings indicate that the ERM cells stimulate the production of MMP-2 in HPDL fibroblasts. Up-regulated MMP-2 activated by MMP-14 in ERM cells could degrade matrix molecules, such as Type IV collagen in the basal membrane between ERM cells and HPDL fibroblasts. We believe that these results are applied to the practical treatment in chronic periodontitis.

Research Products

• [Journal Article] Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament (2010)
  • Author(s): Mitsuru Shimonishi, et al.
  • Journal Title: Journal of Periodontal Research 45巻 Pages: 309-316
  • NAID: 130005009550
  • Peer Reviewed
• [Journal Article] Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament. (2010)
  • Author(s): Mitsuru Shimonishi, et al.
  • Journal Title: Journal of Periodontal Research 45 Pages: 309-316
  • NAID: 130005009550
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Mitsuru Shimonishi, et al.
  • Journal Title: Interface Oral Health Science 2009(Springer) Pages: 123-125
• [Journal Article] Mutual induction of noncollagenous bone proteins at the interface between epithelial cells and fibroblasts from human periodontal ligament (2008)
  • Author(s): Mitsuru Shimonishi, et al.
  • Journal Title: Journal of Periodontal Research 43巻 Pages: 64-75
  • NAID: 10020294808
  • Peer Reviewed
• [Journal Article] Mutual induction of non-collagenous bone proteins at the interface between epithelial cells and fibroblasts from human periodontal ligament (2008)
  • Author(s): Mitsuru Shimonishi, et. al.
  • Journal Title: J Periodont Res 43 Pages: 64-75
  • NAID: 10020294808
  • Peer Reviewed
• [Journal Article] In vitro differentiation of epithelial cells cultured from human periodontal ligament (2007)
  • Author(s): Mitsuru Shimonishi, et al.
  • Journal Title: Journal of Periodontal Research 42巻 Pages: 456-465
  • Peer Reviewed
• [Journal Article] In vitro differentiation of epithelial cells cultured from human periodontal ligament (2007)
  • Author(s): Mitsuru Shimonishi, et. al.
  • Journal Title: J Periodont Res 42 Pages: 456-465
  • Peer Reviewed
• [Presentation] 培養ヒト歯根膜由来上皮細胞および線維芽細胞間におけるMMP-2の誘導 (2009)
  • Author(s): 下西充, 他
  • Organizer: 日本歯周病学会
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-11
• [Presentation] Activation of matrix metaroproteinase (MMP)-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament (2009)
  • Author(s): Mitsuru Shimonishi, et al.
  • Organizer: 第3回インターフェイス口腔健康科学国際シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-01-16
• [Presentation] 培養ヒト歯根膜由来上皮細胞および線維芽細胞間における Bone sialoprotein および Matrix metaroproteinase-2の発現 (2008)
  • Author(s): 下西充, 他
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 富山
  • Year and Date: 2008-11-06
• [Presentation] 培養ヒト歯根膜由来上皮細胞および線維芽細胞間におけるBonesialoproteinおよびMatrix metalloproteinase-2の発現 (2008)
  • Author(s): 下西充、遠藤直樹、齋藤修、小松正志、菊池雅彦
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 富山
  • Year and Date: 2008-11-06
• [Presentation] MMP-2 AND MMP-14 EXPRESSIONS BY EPITHELIAL-MESENCHYMAL INTEACTIONS IN VITRO (2008)
  • Author(s): Mitsuru Shimonishi, et al.
  • Organizer: 86th General Session & Exhibition of the IADR
  • Place of Presentation: トロント (カナダ)
  • Year and Date: 2008-07-03
• [Presentation] MMP-2 and MMP-14 Expressions by Epithelial-mesenchymal Interactions in vitro (2008)
  • Author(s): Mitsuru Shimonishi, et.al.
  • Organizer: International Association of Dental Research
  • Place of Presentation: Canada. Toronto
  • Year and Date: 2008-07-03
• [Presentation] 培養ヒト歯根膜由来上皮細胞および線維芽細胞間における Matrix metaroproteinase-2および-14の発現 (2007)
  • Author(s): 下西充, 他
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 岡山
  • Year and Date: 2007-11-08
• [Presentation] 培養ヒト歯根膜由来上皮細胞および線維芽細胞間における Matrixmetalloproteinase-2および-14の発現 (2007)
  • Author(s): 下西 充、小松 正志
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 岡山
  • Year and Date: 2007-11-08
• [Book] Interface Oral Health Science 2009 (2010)
  • Author(s): Mitsuru Shimonishi, et al.
  • Publisher: Springer 社