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siRNA-mediated highly potent and specific RNAi in human culturedcells and its signals

Research Project

Project/Area Number 19592169
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

YAMATO Kenji  Tokyo Medical and Dental University, 大学院医歯学総合研究科, 講師 (50174751)

Co-Investigator(Kenkyū-buntansha) 西原 達治  九州歯科大学, 歯学部, 教授
西原 達次  九州歯科大学, 歯学部, 教授 (80192251)
Co-Investigator(Renkei-kenkyūsha) 西原 達治  九州歯科大学, 歯学部, 教授
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords実験腫瘍学 / RNAi / siRNA / RNA干渉 / ルシフェラーゼ
Research Abstract

siRNA創薬のためには, 標的遺伝子に対して高いRNAi活性と特異性を有する配列を選択し, これらsiRNAを標的細胞へ効率よく導入しなければならない。現在バイオインフォマティクスを駆使したコンピュータ解析によって理論的に優れたsiRNAの設計が可能となっているが, その効果は実際に生きた細胞を用いて検証しなければならない。本研究ではこの目的のために様々な培養細胞からなるシステムを構築した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (15 results)

All 2009 2008 2007

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (6 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Functional dissection of siRNA sequence by systematic DNA substitution : short interfering DNA-RNA chimera with a DNA seed arm is a powerful tool for mammalian gene silencing with virtually no off-target effect.2009

    • Author(s)
      Ui-Tei, K., Naito, Y., Zenno, S., Nishi, K., Yamato. K., Takahashi, F., Juni, A., Saigo, K.
    • Journal Title

      Nucleic Acids Res. 36

      Pages: 2152-2162

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New highly potent and specific E6 and E7 siRNAs for treatment of HPV16-positive cervical cancer2008

    • Author(s)
      Yamato, K., Yamada, T., Kizaki, M., Ui-Tei, K., Natori, Y., Fujino, M., Nishihara, T., Ikeda, Y., Nasu, Y., Saigo, K., Yoshinouchi, M
    • Journal Title

      Cancer Gene Ther 15

      Pages: 140-153

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional dissection of siRNA sequence by systematic DNA substitution : short interfering DNA-RNA chimera with a DNA seed arm is a powerful tool for mammalian gene silencing with virtually no off-target effect.2008

    • Author(s)
      Ui-Tei, K., Naito, Y., Zenno, S., Nishi, K., Yamato, K., Takahashi, F., Juni, A., Saigo, K
    • Journal Title

      Nucleic Acids Res. 36

      Pages: 2152-2162

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] New highly potent and specific E6 and E7 siRNAs for treatment of HPV16-positive cervical cancer.2008

    • Author(s)
      Yamato, K., et. al.
    • Journal Title

      Cancer Gene Ther. 15

      Pages: 140-153

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional dissection of siRNA sequence by systematic DNA substitution: short interfering DNA-RNA chimera with a DNA seed arm is a powerful tool for mammalian gene silencing with virtually no off-target effect.2008

    • Author(s)
      Ui-Tei, K., et. al.
    • Journal Title

      Nucleic Acids Res. (Electronic publication a head of print)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myeloperoxidase (MPO) is a key regulator of oxidative stress-mediated apoptosis in myeloid leukemic cells2007

    • Author(s)
      Nakazato, T., Sagawa, M., Yamato, K., Xian, M., Yamamoto, T., Suematsu, M, Ikeda, Y., Kizaki, M.
    • Journal Title

      Clin.Cancer Res 13

      Pages: 5436-5445

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Myeloperoxidase (MPO) is a key regulator of oxidative stress-mediated apoptosis in myeloid leukemic cells.2007

    • Author(s)
      Nakazato, T., et. al.
    • Journal Title

      Clin. Cancer Res. 13

      Pages: 5436-5445

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 2本鎖RNA-DNAキメラによるHPV16 E6E7特異的RNAiの誘導Induction of HPV16 E6E7-specific RNAi in cervical cancer cells by double strand RNA-DNA chimeras2008

    • Author(s)
      大和建嗣, 吉野内光夫, 山田健人
    • Organizer
      第67回日本癌学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] 部分的DNA置換によるsiRNA配列の機能解析 : シード領域をDNA に置換したsiRNAはoff-target効果がないDNA-modified siRNA is responsible for significantly reduced off-target effet2008

    • Author(s)
      程久美子, 内藤雄樹, 善野修平, 西賢二, 大和建嗣, 従二綾, 田中愛, 西郷薫
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] 2本鎖RNA-DNAキメラによるHPV16 E6E7特異的RNAiの誘導2008

    • Author(s)
      大和建嗣, 吉野内光夫, 山田健人
    • Organizer
      第6 7回日本癌学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] 部分的DNA置換によるsiRNA配列の機能解析 : シード領域をDNAに置換したsiRNAはoff-target効果がない2008

    • Author(s)
      程久美子, 内藤雄樹, 善野修平, 西賢二, 大和建嗣, 従二綾, 田中愛, 西郷薫
    • Organizer
      第3 1回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] Potent and specific E6 and E7 siRNAs for treatment of HPV16-positive cervical cancer2007

    • Author(s)
      大和建嗣, 吉野内光夫, 山田健人
    • Organizer
      第66回日本癌学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Potent and Specific E6 and E7 siRNAs for treatment of HPV16-positive cervical cancer2007

    • Author(s)
      Yamato, K., et. al.
    • Organizer
      第66回日本癌学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] ヒトパピローマ16型遺伝子を標的とする二本鎖核酸分子およびそれを含む医薬2008

    • Inventor(s)
      大和建嗣, 名取幸和, 山田健人, 吉野内光夫
    • Industrial Property Rights Holder
      大和建嗣, 名取幸和, 山田健人, 吉野内光夫
    • Filing Date
      2008-04-30
    • Related Report
      2008 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ヒトパピローマ1 6型遺伝子を標的とする二本鎖核酸分子およびそれを含む医薬2008

    • Inventor(s)
      大和建嗣, 名取幸和, 山田健人, 吉野内光夫
    • Industrial Property Rights Holder
      東京医科左同
    • Filing Date
      2008-04-30
    • Related Report
      2008 Annual Research Report
    • Overseas

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Published: 2007-04-01   Modified: 2016-04-21  

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