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Analyses of mechanical stress-induced bone formation and metabolism on aging osteoblasts and osteo-like cells

Research Project

Project/Area Number 19592280
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHirosaki University

Principal Investigator

KUSUMI Akinori  Hirosaki University, 大学院・医学研究科, 客員研究員 (90332494)

Co-Investigator(Kenkyū-buntansha) 楠美 智巳  弘前大学, 大学院医学研究科, 講師 (90322932)
佐藤 寿  弘前大学, 医学部附属病院, 助教 (90311539)
榊 宏剛  弘前大学, 医学部附属病院, 助教 (90374850)
Co-Investigator(Renkei-kenkyūsha) 佐藤 寿  弘前大学, 医学部附属病院, 講師 (90311539)
榊 宏剛  弘前大学, 医学部附属病院, 助教 (90374850)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords細胞・組織 / 周期的伸展刺激 / 細胞老化 / MAPK / シグナル伝達 / ヒト骨芽細胞 / 細胞継代・細胞老化 / OPG / MAPK活性化 / RANKL / 組織・細胞 / シグナル伝達経路
Research Abstract

申請者らは、一軸方向周期的伸展刺激(以下、伸展刺激)負荷された正常ヒト骨芽細胞のOPG/RANKL 産生制御について報告(Kusumi A. et al.JBMM23.373-381.2005)してきたが、他の報告とOPG/RANKL産生や、活性化するMAPKの経路に違いがあり、この矛盾の解析が急務であった。
そこで、本研究では、購入した正常ヒト骨芽細胞を継代培養してそれぞれの継代細胞に伸展刺激負荷し、第3継代と第5継代の正常ヒト骨芽細胞について比較解析したところ、第3継代では伸展刺激負荷でp38MAPK経路活性化依存性のOPG産生亢進、RANKL産生抑制制御が認められたが、第5継代では伸展刺激負荷でERK1/2活性化し、OPG産生抑制、RANKL mRNA発現亢進となるが、OPG/RANKL産生制御はERK1/2 経路依存性ではなかった。つまり、申請者らの報告と他の報告との違いの重要なポイントは培養継代の影響である事がわかった。更に、骨芽細胞の継代による細胞老化の方向に研究を向けたかったが、老化関連遺伝子発現の変化は認められなかった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2009 2008 2007 Other

All Journal Article (20 results) (of which Peer Reviewed: 19 results) Presentation (3 results) Book (2 results)

  • [Journal Article] 鬼島宏関節遊離体の臨床病理 : 離断性骨軟骨炎を中心に2009

    • Author(s)
      楠美智巳, 津田英一, 石橋恭之, 楠美昭則
    • Journal Title

      病理と臨床 27

      Pages: 275-282

    • Related Report
      2008 Final Research Report
  • [Journal Article] 関節遊離体の臨床病理 : 離断性骨軟骨炎を中心に2009

    • Author(s)
      楠美智巳, 他
    • Journal Title

      病理と臨床 27

      Pages: 275-282

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Passage.affected competitive regulation of osteogrotegerin synthesis and the receptor activator of nuclear fanor-χ B ligand mRNA expression in normal human osteoblasts stimulated by the application of cyclic tensile strain2009

    • Author(s)
      Kusumi A., et.al.
    • Journal Title

      J Bone Minel Metabolism (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Basic-helix-loop-helix(bHLH)transcription factor DEC2 negatively regulates vascular endothelial growth factore expression2008

    • Author(s)
      Sato F, Bhawal UK, Kawamoto T, FujimotoK, Imaizumi T, Imanaka T, Kondo J, Koyanagi S. Noshiro M. Yoshida H. Kusumi T, Kato Y, Kijima H.
    • Journal Title

      Genes Cells 13

      Pages: 131-144

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical detection of the carcinoma in the specimen obtained radial prostatectomy following hormone therapy2008

    • Author(s)
      Kusumi T. Koie T, Tanaka M, MatsumotoK, Sato F, Kusumi A, Ohyama C, Kijima H.
    • Journal Title

      Pathol Int 58

      Pages: 687-691

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo chemotherapeutic profile of human gallbladder small cell carcinoma2008

    • Author(s)
      Nishime C, Ohnishi Y, Suemizu H, Tamaoki N, Kusumi T, Sato F, Yamazaki H, Nakamura M, Ueyama Y, Kijima H.
    • Journal Title

      Biomed Res 29

      Pages: 251-256

    • NAID

      130004470723

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Claudin-1 expression is induced by tumor necrosis factor-alpha in human pancreatic cancer cells2008

    • Author(s)
      Kondo J, Sato F, Kusumi T, Liu Y, Motonari O, Sato T, Kijima H.
    • Journal Title

      Int J Mol Med 22

      Pages: 645-649

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of transplantable murine osteosarcoma cell line with endochondral ossification2008

    • Author(s)
      Nishi T, Kusumi T, Tanaka M, Sato F, Sasaki M, Kudo H, Kijima H
    • Journal Title

      Anticancer Res 28

      Pages: 1627-1631

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Period is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells by TNF-α2008

    • Author(s)
      Suzuki T, Sato F, Kondo J, Liu Y, Kusumi T, Fujimoto K, Kato Y, Sato T, Kijima H.
    • Journal Title

      Biomed Res 29

      Pages: 99-103

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cardiac tumor comprising two components including typical myxoma and atypical hypercellularity suggesting a malignant change2008

    • Author(s)
      Kusumi T, Minakawa M, Fukui K, Saito S, Ohashi M, Sato F, Fukuda I, Kijima H.
    • Journal Title

      Cardiovasc Pathol 10

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decreased expression of claudin-1 is correlated with recurrence status in esophageal squamous cell carcinoma2008

    • Author(s)
      Miyamoto K, Kusumi T, Sato F, Kawasaki H, Shibata S, Ohashi M, Hakamada K, Sasaki M, Kijima H.
    • Journal Title

      Biomed Res 29

      Pages: 71-76

    • NAID

      130004724051

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical detection of the carcinoma in the specimen obtained radial prostatectomy following hormone therapy2008

    • Author(s)
      Kusumi T., et.al.
    • Journal Title

      Pathol Int 58

      Pages: 687-694

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Basic-helix-loop-helix(bHLH)transcription factor DEC2 negatively regulates vascular endothelial growth factore expression2008

    • Author(s)
      Sato, et. al.
    • Journal Title

      Genes Cells 13

      Pages: 131-144

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of vascular endothelial growth factor by photodynamic therapy with mono-L-aspartyl chlorin e6(NPe6)in oral squamous cell carcinoma2007

    • Author(s)
      Nakagawa H, Matsumiya T, Sakaki H, Imaizumi T, Kubota K, Kusumi A, Kobayashi W, Kimura H.
    • Journal Title

      Oral Oncol 43

      Pages: 544-550

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decreased expression of claudin-1 correlates with recurrence status in breast cancer2007

    • Author(s)
      Morohashi S, Kusumi T, Sato F, Odagiri H, Chiba H, Yoshihara S, Hakamada K, Sasaki M, Kijima H
    • Journal Title

      Int J Mol Med 20

      Pages: 139-143

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Locally administered low-dose alendronate increases bone mineral density during distraction osteogenesis in a rabbit model2007

    • Author(s)
      Omi H, Kusumi T, Kijima H, Toh S
    • Journal Title

      J Bone Joint Surg Br. 89

      Pages: 984-988

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of vascular endothelial growth factor by photodynamic therapy with mono-L-asparty1 chlorin e6(NPe6)in oral squamous cell carcinoma2007

    • Author(s)
      Nakagawa H, et. al.
    • Journal Title

      Oral Oncol 43

      Pages: 544-550

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Decreased expression of claudin-1 correlates with recurrence status in breast cancer2007

    • Author(s)
      Morohashi S, et. al.
    • Journal Title

      Int J Mol Med 20

      Pages: 139-143

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Locally administered low-dose alendronate increases bone mineral density during distraction osteogenesis in a rabbit model2007

    • Author(s)
      Omi H, et. al.
    • Journal Title

      J Bone Joint Surg Br 89

      Pages: 984-988

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Passage-affected competitive regulation of osteoprotegerin synthesis and the receptor activator of nuclear factor- κ B ligand mRNA expression in normal human osteoblasts stimulated by the application of cyclic tensile strain

    • Author(s)
      Kusumi A, Kusumi T, Miura J, Tateishi T.
    • Journal Title

      J Bone Miner Metabolism (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] The influence of culture passages on OPG synthesis and RANKL mRNA expression in normal human osteoblasts stimulated with the application of cyclic tensile strain in itro2007

    • Author(s)
      楠美昭則
    • Organizer
      第37回日本免疫学会総会学術集会.
    • Place of Presentation
      東京都
    • Year and Date
      2007-11-20
    • Related Report
      2008 Final Research Report
  • [Presentation] The influence of culture passages on OPG synthesis and RANKL mRNA expression in normal human osteoblasts stimulated with the application of cyclic tensile strain in vitro2007

    • Author(s)
      楠美 昭則
    • Organizer
      第37回日本免疫学会総会学術集会
    • Place of Presentation
      東京都
    • Year and Date
      2007-11-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] 周期的伸展刺激負荷されたヒト骨芽細胞の培養継代回数によるOPG産生とsRANKL発現制御の影響2007

    • Author(s)
      楠美昭則、他
    • Organizer
      第61回日本口腔科学会学術集会
    • Place of Presentation
      神戸市
    • Year and Date
      2007-04-19
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Book] Nakane A et al eds. Emerging Theories of Host Defense2007

    • Author(s)
      Kusumi A, Sakaki H, Kusumi T, Nakagawa H, Kubota K, Oda M, Satoh H, Kimura H.
    • Total Pages
      301
    • Publisher
      The influence of serial passage on cyclic tensile strain-induced osteoprotegerin synthesis from normal human osteoblasts in vitro. Hirosaki University Press. Hirosaki University Press
    • Related Report
      2008 Final Research Report
  • [Book] Emerging Theories of Host Defense2007

    • Author(s)
      Nakane A, et. al. eds
    • Total Pages
      301
    • Publisher
      Hirosaki University Press
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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