Regulation of genome stability undernutritionalstarvation

• Project/Area Number: 19657039
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Kyoto University
• Principal Investigator: NAKASE Yukiko 京都大学, 放射線生物研究センター, 研究員 (80402923)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO Tomohiro 京都大学, 放射線生物研究センター, センター長 (80212223)
• Project Period (FY): 2007 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥6,138,150 (Direct Cost: ¥5,838,150、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥870,469 (Direct Cost: ¥870,469); Fiscal Year 2009: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2008: ¥1,467,681 (Direct Cost: ¥1,467,681); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 細胞情報伝達機構 / シグナル伝達 / 栄養源枯渇 / TSC / ユビキチンリガーゼ / アミノ酸透過酵素

Research Abstract

The TSC-Rheb signaling pathway plays critical roles in the regulation of cell growthunder various conditions. In Schizosaccharomyces pombe, the amount of amino acid transporters at the cell surface is tightly regulated in response to environmental condition. However, how cellular localization of amino acid transporters is regulated under certain conditions remains obscure. Here we demonstrate that ubiquitin ligasePub1 and arrestin-like protein Any1 coordinate regulation of aminoacid transporters and TSC-Rheb signaling pathway controls this regulation.

Research Products

• [Journal Article] Intracellular trafficking and ubiquitination of the Schizosac charomyces pombe amino acid (2012)
  • Author(s): Nakase M, Nakase Y, ChardwiriyapreechaS, Kakinuma Y, Matsumoto T, Takegawa K
  • Journal Title: Microbiology Volume: 158(Pt 3) Issue: 3 Pages: 659-73
  • DOI: 10.1099/mic.0.053389-0
  • Peer Reviewed
• [Journal Article] Ectopic overproduction of a sporulation-specific transcription factor induces assembly of prespore-like membranous compar tments invegetative cells of fission yeast. (2009)
  • Author(s): Nakase Y, Hirata A, Shimoda C, Nakamura T
  • Journal Title: Genetics Volume: 183(3) Issue: 3 Pages: 1195-9
  • DOI: 10.1534/genetics.109.106906
  • Peer Reviewed
• [Journal Article] Distinctive responses to nitrogen starvation in the dominant active mutants of the fission yeast Rheb GTPase (2009)
  • Author(s): Murai T, Nakase Y, Fukuda K, ChikashigeY, Tsutsumi C, Hiraoka Y, Matsumoto T
  • Journal Title: Genetics Volume: 183(2) Issue: 2 Pages: 517-27
  • DOI: 10.1534/genetics.109.105379
  • Peer Reviewed
• [Journal Article] Ectopic overproduction of a meiosis-specific transcriptional factor induces assembly of prospore-like membranous compartments in fission yeast vegetative cells (2009)
  • Author(s): Yukiko Nakase, Aiko Hirata, Chikashi Shimoda, Taro Nakamura
  • Journal Title: Genetics 7 Pages: 1195-1199
  • Peer Reviewed
• [Journal Article] Distinctive responses to nitrogen starvation in the dominant active mutants of the fission yeast Rheb GTPase (2009)
  • Author(s): Tomoka Murai, Yukiko Nakase, Keiko Fukuda, Yuji Chikashige, Chihiro Tsutsumi, Yasushi Hiraoka, Tomohiro Matsumoto
  • Journal Title: Genetics 20 Pages: 517-527
  • Peer Reviewed
• [Journal Article] Live o bservation of forespore membrane for mation in fission yeast (2008)
  • Author(s): Nakamura T, Asakawa H, Nakase Y, Kashiwazaki J, Hiraoka Y, Shimoda C
  • Journal Title: Mol Biol Cell Volume: 19(8) Issue: 8 Pages: 3544-53
  • DOI: 10.1091/mbc.e08-04-0414
  • Peer Reviewed
• [Journal Article] Meiotic spindle pole bodies acquire the ability to assem ble the spore plasma me mbrane by sequential recruitment of sporulation-specific components in fission yeast (2008)
  • Author(s): Nakase Y, Nakamura-Kubo M, Ye Y, Hirata A, Shimoda C, Nakamura T
  • Journal Title: Mol Biol Cell Volume: 19(6) Issue: 6 Pages: 2476-87
  • DOI: 10.1091/mbc.e08-02-0118
  • Peer Reviewed
• [Journal Article] Meiotic spindle pole bodies acquire the ability to assemble the spore plasma membrane by sequential recruitment of sporulation-speoific components in fission yeast. (2008)
  • Author(s): Nakase Y, Nakamura-Kubo M, Ye Y, Hirata A, Shimoda C, Nakamura T.
  • Journal Title: Molecular Biology of the Cell 19 (6) Pages: 2476-2487
  • Peer Reviewed
• [Journal Article] Live observation of forespore membrane formation in fission yeast. (2008)
  • Author(s): Nakamura T, Asakawa H, Nakase Y, Kashiwazaki J, Hiraoka Y, Shimoda C.
  • Journal Title: Molecular Biology of the Cell 19 (8) Pages: 3544-3553
  • Peer Reviewed
• [Presentation] TSC-Rhebシグナル伝達経路はユビキチンリガーゼPub1とアレスチン様タンパク質Any1の機能を介してアミノ酸トランスポーターの局在を制御している (2012)
  • Author(s): 中瀬由起子、中瀬舞、村井朋香、大坪瑶子、山本正幸、竹川薫、松本智裕
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2012-12-01
• [Presentation] TSC-Rhebシグナル伝達経路はユビキチンリガーゼPub1とアレスチン様タンパク質Any1の機能を介してアミノ酸トランスポーターの局在を制御している (2011)
  • Author(s): 中瀬由起子、中瀬舞、村井朋香、大坪瑶子、山本正幸、竹川薫、松本智裕
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-12-01
• [Presentation] TSC-Rhb-TORシグナル伝達経路におけるアミノ酸透過酵素の制御機構 (2010)
  • Author(s): 中瀬由起子、村井朋香、中瀬舞、竹川薫、松本智裕
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 奈良
  • Year and Date: 2010-09-09
• [Presentation] TSC-Rhb-TORシグナル伝達経路におけるアミノ酸透過酵素の制御機構 (2010)
  • Author(s): 中瀬由起子
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 奈良市 ならまちセンター
  • Year and Date: 2010-09-09
• [Presentation] TSC-Rheb-Tor経路とエンドサイトーシス経路との関わり (2009)
  • Author(s): 中瀬由起子、村井朋香、松本智裕
  • Organizer: 弟9回日本分子生物学会
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] TSC-Rheb-Tor経路とエンドサイトーシス経路との関わり (2009)
  • Author(s): 中瀬由起子
  • Organizer: 日本分子生物学会 弟9回 春期シンポジウム
  • Place of Presentation: 宮崎県シーガイアワールドコンベンションセンター・サミット
  • Year and Date: 2009-05-11