• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

血液RNAを用いた口腔癌の早期診断

Research Project

Project/Area Number 19659531
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionEhime University

Principal Investigator

浜川 裕之  Ehime University, 大学院・医学系研究科, 教授 (20127905)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2008: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywords口腔癌 / 血液RNA / 腫瘍マーカー / 早期診断 / 癌
Research Abstract

口腔顎顔面領域に発生する悪性腫瘍の診断および治療を行う上で、前立腺癌におけるprostate specific antigen(PSA)のような特異度および鋭敏度共に優れた腫瘍マーカーは存在しない。有用性の高い腫瘍マーカーの同定は二次予防の徹底や治療後の病変の残存および再発の診断において極めて重要である。本研究では、ヒト全遺伝子(36,181種類)を解析対象としたマイクロアレイを用いて、口腔癌の血液RNA腫瘍マーカーの同定を試みた。健常者および担癌患者血液3mlよりtotal RNAを抽出し、マイクロアレイによる網羅的遺伝子発現解析を行った。その結果、健常者血液(13検体)には全くその発現が検出されない遺伝子を588種類同定し、そのうち口腔扁平上皮癌患者血液(12検体)において発現亢進が認められた遺伝子を53種類同定した。実際に、T1舌扁平上皮癌患者では治療前の血液RNAでは53種類のうち2種類の遺伝子の発現亢進が検出されたが、治療後の血液RNAではいずれの遺伝子の発現も検出されなかった。さらに、53種類のうち20種類の遺伝子は胃癌、肺癌、大腸癌、乳癌、肝癌、腎癌患者の血液RNAにおいてもその発現亢進が検出された。すなわち、ヒト癌共通の血液RNA腫瘍マーカーの存在が確認された。以上の結果より、血液RNAを用いた癌の早期診断および再発評価が技術的に十分可能であり、血液RNA腫瘍マーカーによる癌のスクリーニング検査の実現の可能性が示唆された。

Report

(2 results)
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (11 results)

All 2008 2007

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (1 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Basic evidence of molecular targeted therapy for oral cancer and salivary gland cancer2008

    • Author(s)
      Hamakawa H, et al.
    • Journal Title

      Head Neck 30

      Pages: 800-809

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of lymph node micrometastases in patients with squamous carcinoma of the head and neck2008

    • Author(s)
      Ferlito A, Rinaldo A, Devaney KO, Nakashiro K, Hamakawa H
    • Journal Title

      Eur Arch Otorhinolaryngol 265

      Pages: 1147-1153

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stat3 as a molecular target in RNA interference-based treatment of oral squamous cell carcinoma2008

    • Author(s)
      Klosek SK, Nakashiro K, Hara S, Goda H, Hamakawa H
    • Journal Title

      Oncol Rep 20

      Pages: 873-878

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma-membrane-anchored growth factor pro-amphiregulin binds A-type lamin and regulates global transcription2008

    • Author(s)
      Isokane M, Hieda M, Hirakawa S, Shudou M, Nakashiro K, Hashimoto K, Hamakawa H, Higashiyama S
    • Journal Title

      J Cell Sci 121

      Pages: 3608-3618

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Akt isoforms in HGF-induced invasive growth of human salivary gland cancer cells2008

    • Author(s)
      Hara S, et. al.
    • Journal Title

      Biochem Biophys Res Commun (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multi-institutional phase II trial of S-1 in patients with oral squamous cell carcinoma2008

    • Author(s)
      Harada K, et. al.
    • Journal Title

      Anticancer Drugs 19

      Pages: 85-90

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth inhibition of human salivary gland tumor cells by introduction of pogesterone receptor and progesterone treatment2007

    • Author(s)
      Yoshimura T, et. al.
    • Journal Title

      Endocrine-Related Cancer 14

      Pages: 1107-1116

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of homozygous deletions of tumor suppressorgene FAT in oral cancer using CGH-array2007

    • Author(s)
      Nakaya K, et. al.
    • Journal Title

      Oncogene 26

      Pages: 5300-5308

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Sentinel node navigation surgery2008

    • Author(s)
      Hamakawa H
    • Organizer
      12th Intemational Congress on Oral Cancer
    • Place of Presentation
      Shanghai, China
    • Year and Date
      2008-05-24
    • Related Report
      2008 Annual Research Report
  • [Book] 口腔内科学(頭頸部の画像の読み方、前癌病変、異形成性病変、色素沈着とメラノーマ)2008

    • Author(s)
      浜川 裕之
    • Publisher
      飛鳥出版室
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 頭頸部癌の腫瘍マーカー2007

    • Inventor(s)
      中城 公一、浜川 裕之
    • Industrial Property Rights Holder
      愛媛大学
    • Industrial Property Number
      2007-203371
    • Filing Date
      2007-08-03
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi