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Crosstalk between cell cycle regulatory mechanism and DNA repair

Research Project

Project/Area Number 19681004
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionKobe University

Principal Investigator

NISHI Ryotaro  Kobe University, 自然科学系先端融合研究環・バイオシグナル研究センター, 助教 (80446525)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords放射線生物影響 / DNA修復 / 細胞周期 / ヌクレオチド除去修復 / 中心体 / 色素性乾皮症
Research Abstract

Nucleotide excision repair (NER) is a versatile DNA repair mechanism that eliminates a wide variety of DNA lesions including UV-induced thymine dimers. XPC complex plays an essential role in DNA damage recognition of NER. Although centrin 2 which belongs to calmodulin super family was suggested to enhance NER by increasing DNA-binding activity of XPC, the precise mechanism of this enhancement remains to be elucidated. Biochemical studies using truncated mutant of centrin 2 indicated that C-terminal domain of centrin 2 is sufficient for enhancement of reconstituted NER and DNA-binding activity of XPC via complex formation with XPC. On the contrary, N-terminal domain interacted with UV-DDB, which is a component of E3 ligase ubiquitylating XPC according to UV irradiation. Electrophoretic mobility shift assay suggested that centrin 2 aids in formation of complex between XPC and UV-DDB on damaged-DNA. Furthermore, UV-C induced ubiquitylation of XPC was compromised in the absence of centrin 2, implying that centrin 2 might regulate ubiquitylation of XPC by interacting with two damage recognition factors via structurally independent two domains.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report

Research Products

(14 results)

All 2009 2008 2007 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Hoeijmakers, Wim Vermeulen, Fumio Hanaoka and Kaoru Sugasawa, UV-DDB-dependent regulation of nucleotide excision repair kinetics in living cells2009

    • Author(s)
      Ryotaro Nishi, Sergey Alekseev, Christoffel Dinant, Deborah Hoogstraten, Adriaan B. Houtsmuller, Jan. H.J.
    • Journal Title

      DNA Repair 8

      Pages: 767-776

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] UV-DDB-dependent regulation of nucleotide excision repair kinetics in living cells2009

    • Author(s)
      Ryotaro Nishi
    • Journal Title

      DNA Repair (未定)(掲載確定)

    • Related Report
      2008 Annual Research Report
  • [Journal Article] In vivo destabilization and functional defects of the xeroderma pigmento sum protein C caused by a pathogenic missense mutation2007

    • Author(s)
      G. Yasuda., R. Nishi
    • Journal Title

      Molecular and Cellular Biology 27

      Pages: 6606-6614

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Centrin 2 mediates interaction between XPC and DDB22009

    • Author(s)
      Ryotaro Nishi
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Centrin 2 mediates interaction between XPC and DDB2.2009

    • Author(s)
      Ryotaro Nishi
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Centrin 2は損傷認識因子XPCとDDB2の相互作用を促進する2009

    • Author(s)
      yotaro Nishi
    • Organizer
      第20回DNA複製・組換え・複製ワークショップ
    • Place of Presentation
      琵琶湖コンファレンスセンター
    • Year and Date
      2009-11-01
    • Related Report
      2009 Final Research Report
  • [Presentation] Centrin 2は損傷認識因子XPCとDDB2の相互作用を促進する2009

    • Author(s)
      Byotaro Nishi
    • Organizer
      第20回DBA複製・組換え・修復ワークショップ
    • Place of Presentation
      琵琶湖コンファレンスセンター(滋賀県)
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Centrin 2 enhances in vitro nucleotide excision repair via complex formation with xeroderma pigmentosum group C protein through its C-termin domain.2008

    • Author(s)
      Ryotaro Nishi
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] Centrin 2 enhances in vitro nucleotide excision repair via complex formation with xeroderma pigmentosum group C protein through its C-terminal domain2008

    • Author(s)
      Ryotaro Nishi
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヌクレオチド除去修復因子の細胞内動態とその制御機構2008

    • Author(s)
      西 良太郎
    • Organizer
      組換え複製合同ワークショップ
    • Place of Presentation
      ラフォーレ修善寺
    • Year and Date
      2008-03-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヌクレオチド除去修復因子の細胞内動態とその制御機構2008

    • Author(s)
      西良太郎
    • Organizer
      第19回DNA複製・分配ワークショップ・合同ワークショップ
    • Place of Presentation
      ラフォーレ修善寺
    • Related Report
      2009 Final Research Report
  • [Presentation] ヌクレオチド除去修復因子の細胞内動態とその制御機構の解析2007

    • Author(s)
      西 良太郎
    • Organizer
      BMB2007
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヌクレオチド除去修復因子の細胞内動態とその制御機構の解析2007

    • Author(s)
      Ryotaro Nishi
    • Organizer
      第30回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.biosig.kobe-u.ac.jp/lab_4.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-03-31   Modified: 2016-04-21  

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