|Budget Amount *help
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2007: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Efficient labeling of peptides, proteins, and antibodies were developed based on the rapid azaelectrocyclization, and the method was applied to the PET and the noninvasive fluorescence imaging. General solid-supported synthesis of N-glycans was established and their dendrimer-type clusters, mimicking the "glycan multivalency effects" in vivo, were efficiently prepared by his own "self-activating" Huisgen 1,3-dipolar cycloaddition. Modification of the proteins and/or the living cell surfaces by N-glycans were also established by rapid azaelectrocyclization. PET and noninvasive fluorescence imaging successfully visualized the trafficking of N-glycoclusters and N-glycan-engineered living cells to the specific organs and/or tumors implanted in living animals.