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Structural studies on intramembrane cleaving proteases

Research Project

Project/Area Number 19687004
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

NOGI Terukazu  Osaka University, 蛋白質研究所, 助教 (40379102)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Keywords構造生物学 / 蛋白質 / シグナル伝達 / 立体構造解析 / 膜蛋白質 / プロテアーゼ / 結晶化 / 組み換え蛋白質の大量発現 / 膜内蛋白質切断
Research Abstract

In this project, we carried out structural and biochemical studies on bacterial intramembrane proteases so as to elucidate the molecular mechanism of the regulated intramembrane proteolysis (RIP). We succeeded in purifying one of the intramembrane proteases with high purity and stability. In addition, we have found that the extracellular region of proteases is involved in the interaction with the substrate in the course of RIP.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (14 results)

All 2009 2008 2007 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification2008

    • Author(s)
      禾晃和, 寒川剛, 田畑早苗, 長江雅倫, 川上恵子, 別府亜耶子, 服部光治, 安井典久, 高木淳一
    • Journal Title

      Protein Science Vol.17

      Pages: 2120-2126

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A murine monoclonal antibody that binds N-terminal extracellular segment of human protease activated receptor-42008

    • Author(s)
      寒川剛, 禾晃和, 高木淳一
    • Journal Title

      Hybridoma Vol.27

      Pages: 331-335

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] キメラ蛋白質を用いた膜内プロテアーゼの基質認識機構の解析2009

    • Author(s)
      禾晃和, 高木淳一
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 細菌型site-2 proteaseの基質認識におけるPDZドメインの役割2009

    • Author(s)
      禾晃和, 高木淳一
    • Organizer
      蛋白研セミナー「膜蛋白質の機能発現メカニズムの解明に向けて-細胞膜上での事象の解析-」
    • Place of Presentation
      大阪大学蛋白質研究所(大阪府)
    • Year and Date
      2009-09-24
    • Related Report
      2009 Final Research Report
  • [Presentation] 細菌型site-2 proteaseの基質認識におけるPDZドメインの役割2009

    • Author(s)
      禾晃和
    • Organizer
      蛋白研セミナー「膜蛋白質の機能発現メカニズムの解明に向けて -細胞膜上での事象の解析-」
    • Place of Presentation
      大阪大学蛋白質研究所(大阪府)
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural analysis of PDZ domains of bacterial intramembrane proteases2009

    • Author(s)
      禾晃和, 高木淳一
    • Organizer
      American Crystallographic Association Annual Meeting Toronto 2009
    • Place of Presentation
      Sheraton City Centre Hotel(カナダ、トロント市)
    • Year and Date
      2009-07-28
    • Related Report
      2009 Final Research Report
  • [Presentation] Structural analysis of PDZ domains of bacterial intramembrane proteases2009

    • Author(s)
      禾晃和
    • Organizer
      American Crystallographic Association, Annual Meeting Toronto 2009
    • Place of Presentation
      Sheraton City Centre Hotel(カナダ、トロント市)
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] ドメインスワッピング変異体を用いた膜内プロテアーゼの基質認識機構の解析2009

    • Author(s)
      禾晃和, 高木淳一
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本全日空ホテル(熊本県)
    • Year and Date
      2009-05-21
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Optimizing detergent-solubilization conditions for structural analysis of site-2 protease family proteins2008

    • Author(s)
      禾晃和, 高木淳一
    • Organizer
      Gordon Research Conference "Proteolytic Enzymes & Their Inhibitors"
    • Place of Presentation
      コルビー=ソーヤー=カレッジ(米国・ニューハンプシャー州)
    • Year and Date
      2008-07-07
    • Related Report
      2009 Final Research Report
  • [Presentation] Optimizing detergent-solubilization conditions for structural analysis of site-2 protease family proteins2008

    • Author(s)
      禾晃和
    • Organizer
      Gordon Research ConferenceProteolytic enzymes & their inhibitors
    • Place of Presentation
      Colby-Sawyer College(NH, USA)
    • Related Report
      2008 Annual Research Report
  • [Presentation] 抗GPCRモノクローナル抗体20.1を用いたアフィニティータグシステムの開発I-抗体策作成とエピトープ解析2007

    • Author(s)
      寒川, 剛
    • Organizer
      第7回日本蛋白質科学会年会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2007-05-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] 抗GPCRモノクローナル抗体20.1を用いたアフィニティータグシステムの開発II-Fabフラグメントの結晶構造解析2007

    • Author(s)
      禾, 晃和
    • Organizer
      第7回日本蛋白質科学会年会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2007-05-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] 抗GPCR抗体20.1を用いたアフィニティータグシステムの開発:(III 高親和性ぺプチド配列の探索2007

    • Author(s)
      田畑, 早苗
    • Organizer
      第7回日本蛋白質科学会年会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2007-05-25
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.protein.osaka-u.ac.jp/rcsfp/synthesis/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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