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Dynamic analysis of transport vesicle formation using artificial planar lipid bilayer

Research Project

Project/Area Number 19687008
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

SATO Ken  The University of Tokyo, 大学院・総合文化研究科, 准教授 (00303602)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2007: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Keywords小胞輸送 / 輸送小胞 / 小胞体 / 低分子量GTPase / 出芽酵母 / 1分子計測 / プロテオリポソーム / GTPase
Research Abstract

We investigated the dynamics of fluorescently labeled cargo proteins during COPII vesicle formation using single-molecule microscopy combined with an artificial planar lipid bilayer. We show that continuous GTPase cycles of Sar1 facilitate cargo concentration into COPII vesicle buds. We propose that the minimal set of COPII components is required to concentrate cargo molecules.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report

Research Products

(25 results)

All 2010 2009 2008 2007

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (7 results) Book (6 results)

  • [Journal Article] Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3.2010

    • Author(s)
      Yamashita, M., Kurokawa, K., Sato, Y., Yamagata, A., Mimura, H., Yoshikawa, A. Sato, K., Nakano, A., Fukai, S.
    • Journal Title

      Nature Struct. &Mol. Biol. 17

      Pages: 180-186

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec32010

    • Author(s)
      Yamashita, M., et al.
    • Journal Title

      Nature Struct & Mel.Biol. 17

      Pages: 180-186

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane.2009

    • Author(s)
      Tabata, K.V., Sato, K., Ide, T., Nishizaka, T., Nakano, A., Noji, H.
    • Journal Title

      EMBO J. 28

      Pages: 3279-3289

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] O-Mannosylation is required for the solubilization of heterologously expressed human b-amyloid precursor protein in Saccharomyces cerevisiae.2009

    • Author(s)
      Murakami-Sekimata, A., Sato, K., Sato, K., Takashima, A., Nakano, A.
    • Journal Title

      Genes Cells 14

      Pages: 205-215

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane2009

    • Author(s)
      Tabata, K.V., et al.
    • Journal Title

      EMBO J. 28

      Pages: 3279-3289

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] O-Mannosylation is required for the solubilization of heterologously expressed human b-amyloid precursor protein in S. cerevisiae2009

    • Author(s)
      Murakami-Sekimata, A. et.al.
    • Journal Title

      Genes Cells 14

      Pages: 205-215

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Smy2p participates in COPII vesicle formation through the interaction with Sec23p/Sec24p subcomplex.2008

    • Author(s)
      Higashio, H., Sato, K., Nakano, A.
    • Journal Title

      Traffic 9

      Pages: 79-93

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Smy2p participates in COPII vesicle formation through the interaction with Sec23p/Sec24p subcomplex2008

    • Author(s)
      Hironori Higashio, et. al.
    • Journal Title

      Traffic 9

      Pages: 79-93

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms of COPII vesicle formation and protein sorting.2007

    • Author(s)
      Sato, K., Nakano, A.
    • Journal Title

      FEBS Lett. 581

      Pages: 2076-2082

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] VPS9a, the common activator for two distinct types of Rab5 GTPases, is essential for the development of Arabidopsis thaliana.2007

    • Author(s)
      Goh, T., Uchida, W., Arakawa, S., Ito, E., Dainobu, T., Ebine, K., Takeuchi, M., Sato, K., Ueda, T., Nakano, A.
    • Journal Title

      Plant Cell 19

      Pages: 3504-3515

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms of COPII vesicle formation and protein sorting2007

    • Author(s)
      Ken Sato, et. al.
    • Journal Title

      FEBS Lett. 581

      Pages: 2076-2082

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VPS9a, the common activator for two distinct types of Rab5 GTPases, is essential for the development of Arabidopsis thaliana2007

    • Author(s)
      Tatsuaki Goh, et. al.
    • Journal Title

      Plant Cell 19

      Pages: 3504-3515

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 小胞体における輸送小胞形成の分子メカニズムの解析2009

    • Author(s)
      佐藤健
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-21
    • Related Report
      2009 Final Research Report
  • [Presentation] Dynamics of COPII-mediated cargo concentration observed in a planar lipid membrane2008

    • Author(s)
      Ken Sato
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2008-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] Sed4pによるSar1pの制御機構の解析2008

    • Author(s)
      小寺千絵、中野明彦、佐藤健
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2008-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] Dynamics of COPII-mediated cargo concentration observed in a planar lipid membrane2008

    • Author(s)
      Ken Sato
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Sed4pによるSar1pの制御機構の解析2008

    • Author(s)
      小寺千絵, ほか
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Cargo concentration and COPII vesicle formation observed in reconstituted planar model membrane system2007

    • Author(s)
      Ken Sato
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Year and Date
      2007-12-13
    • Related Report
      2009 Final Research Report
  • [Presentation] Cargo concentration and COPII vesicle formation observed in reconstituted planar model membrane system2007

    • Author(s)
      Ken Sato
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Book] 細胞工学(Sar1が制御する輸送小胞形成の分子メカニズム)2009

    • Author(s)
      佐藤健
    • Publisher
      秀潤社
    • Related Report
      2009 Final Research Report
  • [Book] 細胞工学「Sar1が制御する輸送小胞形成の分子メカニズム」2009

    • Author(s)
      佐藤健
    • Total Pages
      5
    • Publisher
      秀潤社
    • Related Report
      2009 Annual Research Report
  • [Book] The Golgi Apparatus-COPII2008

    • Author(s)
      Ken Sato, Akihiko Nakano
    • Publisher
      Springer
    • Related Report
      2009 Final Research Report
  • [Book] 蛋白質 核酸 酵素(小胞体における積み荷蛋白質の選別とCOPII小胞京成のメカニズム)2008

    • Author(s)
      佐藤健
    • Publisher
      共立出版
    • Related Report
      2009 Final Research Report
  • [Book] The Golgi Apparatus2008

    • Author(s)
      Sato, K. et.al.
    • Publisher
      Springer Wien New York
    • Related Report
      2008 Annual Research Report
  • [Book] 小胞体における積み荷蛋白質の選別とCOPII小胞形成のメカニズム2008

    • Author(s)
      佐藤健
    • Publisher
      共立出版
    • Related Report
      2008 Annual Research Report

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Published: 2007-03-31   Modified: 2016-04-21  

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