Dynamic analysis of transport vesicle formation using artificial planar lipid bilayer
| Project/Area Number |
19687008
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATO Ken The University of Tokyo, 大学院・総合文化研究科, 准教授 (00303602)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2007: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
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| Keywords | 小胞輸送 / 輸送小胞 / 小胞体 / 低分子量GTPase / 出芽酵母 / 1分子計測 / プロテオリポソーム / GTPase |
|---|
| Research Abstract |
We investigated the dynamics of fluorescently labeled cargo proteins during COPII vesicle formation using single-molecule microscopy combined with an artificial planar lipid bilayer. We show that continuous GTPase cycles of Sar1 facilitate cargo concentration into COPII vesicle buds. We propose that the minimal set of COPII components is required to concentrate cargo molecules.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3.2010
Author(s)
Yamashita, M., Kurokawa, K., Sato, Y., Yamagata, A., Mimura, H., Yoshikawa, A. Sato, K., Nakano, A., Fukai, S.
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Journal Title
Nature Struct. &Mol. Biol. 17
Pages: 180-186
Related Report
Peer Reviewed
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[Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane.2009
Author(s)
Tabata, K.V., Sato, K., Ide, T., Nishizaka, T., Nakano, A., Noji, H.
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Journal Title
EMBO J. 28
Pages: 3279-3289
Related Report
Peer Reviewed
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[Journal Article] VPS9a, the common activator for two distinct types of Rab5 GTPases, is essential for the development of Arabidopsis thaliana.2007
Author(s)
Goh, T., Uchida, W., Arakawa, S., Ito, E., Dainobu, T., Ebine, K., Takeuchi, M., Sato, K., Ueda, T., Nakano, A.
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Journal Title
Plant Cell 19
Pages: 3504-3515
Related Report
Peer Reviewed
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