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Biochemical dissection of miRNA functions

Research Project

Project/Area Number 19687011
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionThe University of Tokyo

Principal Investigator

TOMARI Yukihide  The University of Tokyo, 分子細胞生物学研究所, 准教授 (90447368)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2007: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Keywords核酸 / 蛋白質 / 発現制御 / 生体分子 / 遺伝子 / microRNA / Argonaute / RISC / 小分子RNA / small RNA / miRNA / siRNA / 翻訳抑制 / ショウジョウバエ
Research Abstract

We find that Ago1 represses translation primarily by ATP-dependent shortening of the poly(A) tail of its mRNA targets. Ago1 can also secondarily block a step after cap recognition. In contrast, Ago2 competitively blocks the interaction of eIF4E with eIF4G and inhibits the cap function. Thus, the two Ago proteins in flies regulate translation by different mechanisms.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (14 results)

All 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (10 results)

  • [Journal Article] Argonaute-mediated translational repression (and activation)2009

    • Author(s)
      岩崎信太郎、泊幸秀
    • Journal Title

      Fly 14

      Pages: 205-208

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila Argonautel and Argonaute2 employ distinct mechanisms for translational repression.2009

    • Author(s)
      岩崎信太郎、川俣朋子、泊幸秀
    • Journal Title

      Molecular Cell 34

      Pages: 58-67

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila Argonautel and Argonaute2 employ distinct mechanisms for translational repression2009

    • Author(s)
      岩崎信太郎、川俣朋子、泊幸秀
    • Journal Title

      Molecular Cell 34

      Pages: 58-67

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Argonaute-mediated translational repression(and activation)2009

    • Author(s)
      岩崎信太郎、泊幸秀
    • Journal Title

      Fly 14

      Pages: 205-208

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Mechanisms of translational repression by miRNAs2009

    • Author(s)
      泊幸秀
    • Organizer
      RNA Silencing 2009 : Mechanisms and Applications
    • Place of Presentation
      中国(香港)
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] microRNAによる翻訳抑制機構2009

    • Author(s)
      泊幸秀
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] Mechanisms of translational repression by miRNAs.2009

    • Author(s)
      泊幸秀
    • Organizer
      RNA Silencing 2009: Mechanisms and Applications
    • Place of Presentation
      香港、中国
    • Related Report
      2009 Final Research Report
  • [Presentation] microRNAによる翻訳抑制機構2009

    • Author(s)
      泊幸秀
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜、日本
    • Related Report
      2009 Final Research Report
  • [Presentation] Drosophila Argonautel and Argonaute2 employ distinct mechanisms for translational repression2008

    • Author(s)
      泊幸秀
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学学会大会・合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Biochemical dissection of RISC assembly and function2008

    • Author(s)
      泊幸秀
    • Organizer
      第10回日本RNA学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-07-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] Drosophila Argonautel and Argonaute2 employ distinct mechanisms for translational repression.2008

    • Author(s)
      泊幸秀
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学学会大会・合同大会
    • Place of Presentation
      神戸、日本
    • Related Report
      2009 Final Research Report
  • [Presentation] Biochemical dissection of RISC assembly and function2008

    • Author(s)
      泊幸秀
    • Organizer
      第10回日本RNA学会年会
    • Place of Presentation
      札幌、日本
    • Related Report
      2009 Final Research Report
  • [Presentation] Drosophila Argonaute proteins employ distinct mechanisms for translational repression.2008

    • Author(s)
      岩崎信太郎、川俣朋子、泊幸秀
    • Organizer
      Keystone Symposium (RNAi, MicroRNA, and Non-Coding RNA)
    • Place of Presentation
      Whistler, CANADA
    • Related Report
      2009 Final Research Report
  • [Presentation] Drosophila Argonaute proteins employ distinct mechanisms for translational repression2008

    • Author(s)
      岩崎信太郎、川俣朋子、泊幸秀
    • Organizer
      Keystone symposium (RNAi, MicroRNA, and Non-Coding RNA)
    • Place of Presentation
      Whistler, Canada
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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