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The role of macropinocytosis in repulsive axon guidance

Research Project

Project/Area Number 19700302
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Neuroscience in general
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KABAYAMA Hiroyuki  The Institute of Physical and Chemical Research, 発生神経生物研究チーム, 客員研究員 (10332339)

Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,920,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥800,000 (Direct Cost: ¥800,000)
Keywordsマクロピノサイトーシス / 成長円錐退縮 / 反発性軸索誘導 / syntaxin1B / Neurotoxin C1 / Syntaxin1 / 反発性軸策誘導 / セマフォリン / カルシウム / 軸索ガイダンス / カルシウムストア / 神経突起伸長
Research Abstract

Growth cone collapse is a crucial process for repulsive axon guidance and is accompanied by a reduction in growth cone surface area. This process of reduction may be regulated by endocytosis; however, its molecular mechanism is unclear. Macropinocytosis is a clathrin-independent form of endocytosis in which large areas of plasma membrane can be engulfed. We have reported previously that macropinocytosis is induced in growth cones of chick dorsal root ganglion neurons by semaphorin 3A (Sema3A), a repulsive axon guidance cue, and that Sema3A-induced reduction in growth cone surface area and macropinocytic vacuole area were correlated, suggesting a positive role for macropinocytosis in Sema3A-induced growth cone collapse. In the present study, we found that syntaxin 1B (Syx1B), a membrane trafficking protein, is a negative regulator of macropinocytosis, and its expression is downregulated by Sema3A signaling. Macropinocytosis inhibitor ethylisopropylamiloride or Syx1B overexpression suppressed Sema3A-induced macropinocytosis and growth cone collapse. These results indicate that Syx1B couples macropinocytosis-mediated massive internalization of the plasma membrane to Sema3A-induced growth cone collapse.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (17 results)

All 2011 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (6 results) Remarks (3 results)

  • [Journal Article] Syntaxin 1B suppresses macropinocytosis and Sema3a-induced growth cone collapse.2011

    • Author(s)
      Kabayama H, Takeuchi M, Taniguchi M, Tokushige N, Kozaki S, Mizutani A, Mikoshiba K.
    • Journal Title

      The Journal of Neuroscience. 31(20)

      Pages: 7357-7364

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inositol 1, 4, 5-trisphosphate receptor interacts with the SNARE domain of syntaxin 1B.2011

    • Author(s)
      Tanaka S, Kabayama H, Enomoto M, Saito N, Mikoshiba K.
    • Journal Title

      Journal of Physiological Sciences. [Epub ahead of print] PMID : 21424589

    • NAID

      10029050147

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dissociation of inositol polyphosphates from the C2B domain of synaptotagmin facilitates spontaneous release of catecholamines in adrenal chromaffin cells.A suggestive evidence of a fusion clamp by synaptotagmin.2011

    • Author(s)
      Sasakawa N, Ohara-Imaizumi M, Fukuda M, Kabayama H, Mikoshiba K, Kumakura K.
    • Journal Title

      Neuropharmacology. [Epub ahead of print]PMID : 21402086

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ca^<2+> induces macropinocytosis via F-actin depolymerization during growth cone collapse.2009

    • Author(s)
      Kabayama H, Nakamura T, Takeuchi M, Iwasaki H, Taniguchi M, Tokushige N, Mikoshiba K.
    • Journal Title

      Molecular and Cellular Neuroscience. 40(1)

      Pages: 27-38

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse.2009

    • Author(s)
      Kabayama H, Nakamura T, Takeuchi M, Iwasaki H, Taniguchi M, Tokushige N, Mikoshiba K.
    • Journal Title

      Molecular and Cellular Neuroscience 40

      Pages: 27-38

    • Related Report
      2009 Self-evaluation Report 2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Syntaxin 6 regulates nerve growth factor-dependent neurite outgrowth2008

    • Author(s)
      Kabayama H, Tokushige N, Takeuchi M, Mikoshiba K.
    • Journal Title

      Neuroscience Letters. 436(3)

      Pages: 340-344

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Syntaxin 6 regulates nerve growth factor-dependent neurite outgrowth.2008

    • Author(s)
      Kabayama H, Tokushige N, Takeuchi M, Mikoshiba K.
    • Journal Title

      Neuroscience Letters 436

      Pages: 340-344

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Syntaxin 6 regulates nerve growth factor-dependent neurite outgrowth2008

    • Author(s)
      Kabayama H, Tokushige N, Takeuchi M, Mikoshiba K.
    • Journal Title

      Neuroscience Letters 436

      Pages: 340-344

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse2009

    • Author(s)
      樺山博之、中村健、竹内誠、岩崎広英、谷口雅彦、徳重直子、御子柴克彦
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜 パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse2009

    • Author(s)
      樺山博之、中村健、竹内誠、岩崎宏英、谷口雅彦、徳重直子、御子柴克彦
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse2009

    • Author(s)
      樺山博之、中村健、竹内誠、岩崎広英、谷口雅彦、徳重直子、御子柴克彦
    • Organizer
      第32回 日本分子生物学会
    • Place of Presentation
      横浜パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse2009

    • Author(s)
      樺山博之、中村健、竹内誠、岩崎広英、谷口雅彦、徳重直子、御子柴克彦
    • Organizer
      第36回国際生理学会
    • Place of Presentation
      京都 国立京都国際会館
    • Year and Date
      2009-07-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse2009

    • Author(s)
      樺山博之、中村健、竹内誠、岩崎宏英、谷口雅彦、徳重直子、御子柴克彦
    • Organizer
      第36回国際生理学会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2009-07-30
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse2009

    • Author(s)
      樺山博之、中村健、竹内誠、岩崎広英、谷口雅彦、徳重直子、御子柴克彦
    • Organizer
      第36回国際生理学会
    • Place of Presentation
      京都国立京都国際会館
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等 理化学研究所ホームページ

    • URL

      http://www.riken.go.jp/r-world/info/release/press/2011/110518/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 発生神経生物研究チームホームページ

    • URL

      http://www.riken.go.jp/r-world/research/lab/nokagaku/develop/develop/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 科学技術振興機構ホームページ

    • URL

      http://www.jst.go.jp/pr/announce/20110518/index.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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