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Fundamental study for controlling a food environment against intestinal protozoa contaminated in tap water

Research Project

Project/Area Number 19700609
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Eating habits, studies on eating habits
Research InstitutionOsaka Joshigakuen Junior College

Principal Investigator

MATSUBAYASHI Makoto  Osaka Joshigakuen Junior College, 准教授 (00321076)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords食環境 / 水道水 / クリプトスポリジウム / 下痢 / 原虫 / コクシジウム / C. parvum / 侵入 / 家畜 / Cryptosporidium andersoni / 牛 / Cryptosporidium parvum / C.andersoni / C.parvum
Research Abstract

Host range as a reservoir about Cryptosporidium, Cyclospora, and Giardia, and invasive process of the Parasites into host cells remains unsolved. To obtain fundament data about these unresolved issues, I examined feces of domestic animals including cattle and pigs, and detected parasites were genetically identified. And to clarify the invasive process of Cryptosporidium, I analyzed the zoites of invasive stage in detail. As a result, of 213 cattle samples from 21 prefectures, Cryptosporicliunispp., Eilneria spp., Capillaria bovis, and Trichuris spp. infections were detected in 13 (6.1%), 163 (76.5%), 15 (7.0%), and 8 (3.8%), respectively. All the isolates of Cryptosporidium spp. were genetically identified as C.andersoni, and biologically identified as a novel type. Of 129 pig samples from 8 prefectures, Eimeria spp., Trichuris suis, Ascaris sumn, and Metastrongylus spp. infections were found in 52 pigs (40.3%), 32 (24.8%), 19 (14.7%), and 3 (2.3%), respectively. Our results indicate that environmentally resistant eggs or oocysts of the parasites are widespread on Japanese farms. By ultramicroscopy of C.parvum zoites, three apical rings, an electron-dense collar, and two thick central microtubules were seen at the apical end of sporozoites. C.parvum a tuhulin antigen was 50-kDa molecular weight by western blotting and these apical cytoskeletal structures observed by electron microscopy were composed of a tubul in by immunoelectron microscopy. It was found that infectivity in vitro of this sporozoite, declined within 2hr, suggesting that invasion into host cells after excystation was rapidly accompanied.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (19 results)

All 2010 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 6 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Morphological changes and viability of Cryptosporidium parvum sporozoites after excystation in cell-free culture media2010

    • Author(s)
      Matsubayashi M.et al.
    • Journal Title

      Parasitology (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Coprological survey of parasitic infections in pigs and cattle in slaughterhouse in Osaka2009

    • Author(s)
      Matsubayashi M., Kita T., Narushima T., Kimata I., Tani H., Sasai K., Baba E.
    • Journal Title

      Japan. Journal of Veterinary Medical Science. 71

      Pages: 1079-83

    • Related Report
      2009 Final Research Report
  • [Journal Article] Coprological survey of parasitic infections in pigs and cattle in slaughterhouse in Osaka, Japan2009

    • Author(s)
      Matsubayashi M.et al.
    • Journal Title

      Journal of Veterinary Medical Science 71

      Pages: 1079-83

    • NAID

      130000134457

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electron microscopic observation of cytoskeletal frame structures and detection of tubulin on the apical region of Cryptosporidium parvm sporozoites.2008

    • Author(s)
      Matsubayashi M, Takase H, Kimata I, Nakagawa H, Tani H, Sasai K, Baba E.
    • Journal Title

      Parasitology. 135

      Pages: 295-301

    • Related Report
      2009 Final Research Report
  • [Journal Article] Characterization of monoclonal antibodies that recognize the Eimeria tenella microneme protein MIC22008

    • Author(s)
      Sasai K., Fetterer R.H., Lillehoj H., Matusra S., Constantinoiu C.C., Matsubayashi M., Tani H., Baba E.
    • Journal Title

      Journal of Parasitology. 94

      Pages: 1432-4

    • Related Report
      2009 Final Research Report
  • [Journal Article] Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan.2008

    • Author(s)
      Matsubayashi M., Nagano S., Kita T., Narushima T., Kimata I., Iseki M., Hajiri T., Tani H., Sasai K., Baba E.
    • Journal Title

      Veterinary Parasitology. 158

      Pages: 44-50

    • Related Report
      2009 Final Research Report
  • [Journal Article] Characterization of monoclonal antibodies that recognize the Eimeria tenella microneme protein MIC22008

    • Author(s)
      Sasai K., et.al.
    • Journal Title

      Journal of Parasitology 94(6)

      Pages: 1432-4

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan2008

    • Author(s)
      Matsubayashi M., et.al.
    • Journal Title

      Veterinary Parasitology 158(1-2)

      Pages: 44-50

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electron microscopic observation of cytoskeletal frame structures and detection of tubulin on the apicar region of Cryptosporidium parvu sporozoites.2008

    • Author(s)
      Matsubayashi M., et. al.
    • Journal Title

      Parasitology 135(Pt3)

      Pages: 295-301

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of a mixed infection of a novel Cryolosporidium andersoni and its subgenotype in Japanese cattle.2007

    • Author(s)
      Nagano S., Matsubayashi M., Kita T., Narushima T., Kimata I., Iseki M., Hajiri T., Tani H., Sasai K. Baba E.
    • Journal Title

      Veterinary Parasitology. 149

      Pages: 213-218

    • Related Report
      2009 Final Research Report
  • [Journal Article] Detection of a mixed infection of a novel Cryptosporidimi andersoni and its siibgenotype in Japanese cattle.2007

    • Author(s)
      Nagano S., et. al.
    • Journal Title

      Veterinary Parasitology 149(3-4)

      Pages: 213-218

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological changes and viability of Cryplosporidium parvum sporozoites after excystation in cell-free culture media.

    • Author(s)
      Matsubayashi M., Ando H., Kimata I., Nakagawa H., Furuya M., Tani H. Sasai K.
    • Journal Title

      Parasitology. (In press)

    • Related Report
      2009 Final Research Report
  • [Presentation] Cryptosporidium andersoni novel type の成熟および哺乳マウスにおける感染性について2009

    • Author(s)
      松林, ら
    • Organizer
      第65回日本寄生虫学会西日本支部大会
    • Place of Presentation
      大阪市立大学
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cryptosporidium andersoni novel typeの成熟および哺乳マウスにおける感染性について2009

    • Author(s)
      松林誠. 長野早希、木俣勲、木太俊雅、古家優、谷浩行、笹井和美
    • Organizer
      第65回日本寄生虫学会西日本支部大会
    • Related Report
      2009 Final Research Report
  • [Presentation] Cryptosporidiumの細胞骨格構造の電子顕微鏡学的解析2008

    • Author(s)
      松林誠、高瀬弘嗣、木俣勲、中川英紀、谷浩行、笹井和美、馬場栄一郎
    • Organizer
      第145回日本獣医学会学術大会
    • Related Report
      2009 Final Research Report
  • [Presentation] Molecular Characterization of the Novel Type of Cryptosporidium andersoni Detected in Japan.2008

    • Author(s)
      Matsubayashi M., Nagano S., Kita T., Narushima T. Isao Kimata I., Iseki M., Hajiri T., Tani H., Sasai K., Baba E.
    • Organizer
      16th Japanese-German Cooperative Syinposium on Protozoan Disease
    • Place of Presentation
      Program and Abstracts of Papers
    • Related Report
      2009 Final Research Report
  • [Presentation] Molecular Characterization of the Novel Type of Cryptosporidium andersoni Detected in Japan2008

    • Author(s)
      Matsubayashi M., et.al.
    • Organizer
      16th Japanese-German Cooperative Symposium on Protozoan Disease
    • Place of Presentation
      Gottingen Germany
    • Related Report
      2008 Annual Research Report
  • [Presentation] Cryptosporidium の細胞骨格構造の電子顕微鏡学的解析2008

    • Author(s)
      松林 誠ら
    • Organizer
      第145回日本獣医学会学術集会
    • Place of Presentation
      麻布大学(神奈川県相模原市)
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.oyg.ac.jp/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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