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Screening of the candidate gene involved in clinically relevant radioresistance

Research Project

Project/Area Number 19710049
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionTohoku University

Principal Investigator

KUWAHARA Yoshikazu  Tohoku University, 加齢医学研究所, 助教 (00392225)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywords放射線耐性 / 放射線療法 / 遺伝子発現 / コロニー形成法 / 肝癌 / 放射線
Research Abstract

放射線療法時に見られる放射線耐性細胞は予後を左右する重要な因子の1つである。本研究では、2Gy/dayのX線を30日以上照射し続けても増殖する放射線耐性細胞株の樹立を試み、解析した。その結果、放射線耐性細胞では効率的にX線で誘発されるDNA2本鎖切断を修復している事が分かった。また、PCR based differential display法による遺伝子発現解析から、放射線耐性細胞に特異的に発現が変動している遺伝子が幾つか得られた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (56 results)

All 2009 2008 2007 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (35 results) Remarks (3 results)

  • [Journal Article] Long incubation period for the induction of cancer by thorotrast is attributed to the uneven irradiation of liver cells at the microscopic level.2009

    • Author(s)
      Yamamoto Y, Usuda N, Takatsuji T, Kuwahara Y, Fukumoto M
    • Journal Title

      Radiat Res 171

      Pages: 494-503

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinically relevant radioresistant cells efficiently repair DNA double-strand breaks induced by X-rays2009

    • Author(s)
      Kuwahara Y, Li L, Baba T, Nakagawa H, Shimura T, Yamamoto Y, Ohkubo Y, Fukumoto M
    • Journal Title

      Cancer Sci 100

      Pages: 747-752

    • NAID

      10025144817

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stabilities of 67Ga- and 111In-labeled transferrin in vitro2009

    • Author(s)
      Ohtake Y, Maruko A, Kuwahara Y, Fukumoto M, Ohkubo Y
    • Journal Title

      Protein Pept Lett 16

      Pages: 138-142

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long incubation period from the induction of cancer by Thorotrast is at tributed to the uneven irradiation of liver cells at the microscopic level2009

    • Author(s)
      Yamamoto Y., Usuda N., Takatsuji T., Kuwahara Y., Fukumoto M.
    • Journal Title

      Radiation Research 171

      Pages: 494-503

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinically relevant radioresistant cells efficiently repair DNA double-stra nd breaks induced by X-rays2009

    • Author(s)
      Kuwahara Y., Li L., Baba T., Nakagawa H, Shimura T, Yamamoto Y, Ohkubo Y, Fukumoto M.
    • Journal Title

      Cancer Science 100

      Pages: 747-752

    • NAID

      10025144817

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stabilities of 67Ga- and 111In-labeled transferrin in vitro2009

    • Author(s)
      Ohtake Y, Maruko A., Kuwahar a K., Fukumoto M., Ohkubo Y.
    • Journal Title

      Protein Peptid Letter 16

      Pages: 138-142

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of gene and protein expression of cytochrome P450 and stress-associated molecules in rat liver after spaceflight2008

    • Author(s)
      Baba T, Nishimura M, Kuwahara Y, Ueda N, Naitoh S, Kume M, Yamamoto Y, Fujita J, Funae Y, Fukumoto M
    • Journal Title

      Pathol Int 58

      Pages: 589-595

    • NAID

      10027377866

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neutrophil gelatinase-associated lipocalin acts as a protective factor against H2O2 toxicity2008

    • Author(s)
      Roudkenar MH, Halabian R, Ghasemipour Z, Roushandeh AM, Rouhbakhsh M, Nekogoftar M, Kuwahara Y, Fukumoto M, Shokrgozar MA
    • Journal Title

      Arch Med Res 396

      Pages: 560-566

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profiles in mouse liver cells after exposure to different types of radiation2008

    • Author(s)
      Roudkenar MH, Li L, Baba T, Kuwahara Y, Nakagawa H, Wang L, Kasaoka S, Ohkubo Y, Ono K, Fukumoto M
    • Journal Title

      J Radiat Res 49

      Pages: 29-40

    • NAID

      110006570525

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of apoptosis on K562 cell line and double strand breaks on colon cancer cell line expressing high affinity receptor for granulocyte macrophage-colony stimulating factor (GM-CSF).2008

    • Author(s)
      Roudkenar MH, Bouzari S, Kuwahara Y, Roushandeh AM, Baba T, Oloomi M, Fukumoto M
    • Journal Title

      Iran Biomed J 12

      Pages: 1-6

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of gene and protein expression of cytochrome P450 and stress-associated molecules in rat liver after spaceflight2008

    • Author(s)
      Baba T, Nishimura M, Kuwahar a Y, Ueda N, Naitoh S, Kume M, Yamamoto Y, Fujita J, Funa e Y, Fukumoto M.
    • Journal Title

      Pathology International 58

      Pages: 589-595

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profiles in mouse liver cells after exposure to different types of radiation.2008

    • Author(s)
      Roudkenar MH.
    • Journal Title

      J Radiat Res. 49

      Pages: 29-40

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of apoptosis on K562 cell line and Double strand breaks on colon cancer cell line expressing high affinity receptor for Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF).2008

    • Author(s)
      Roudkenar MH.
    • Journal Title

      Iranian Biomedical J. 12

      Pages: 1-6

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Common Deletion (CD) and a novel deletion of mitochondrial DNA induced by ionizing radiation2007

    • Author(s)
      Wang L, Kuwahara Y, Li L, Baba T, Shin RW, Ohkubo Y, Ono K, Fukumoto M
    • Journal Title

      Int J Radiat Biol 83

      Pages: 433-442

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative stress induced Lipocalin 2 gene expression : addressing its expression under the harmful conditions2007

    • Author(s)
      Roudkenar MH, Kuwahara Y, Baba T, Roushandeh AM, Ebshima S, Abe S, Ohkubo Y, Fukumoto M
    • Journal Title

      J Radiat Res 48

      Pages: 39-44

    • NAID

      110006570515

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of GM-CSF receptors by real-time RT-PCR on cell lines expressing high and low affinity receptors and their relation to cytotoxic effect of chimeric protein (StxA1-GM-CSF)2007

    • Author(s)
      Roudkenar MH, Bouzari S, Oolomi M, Kuwahara Y, Roushandeh AM, Baba T, Fukumoto M
    • Journal Title

      DARU 15

      Pages: 151-155

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Common Deletion (CD) and a novel deletion of mitochondrial DNA induced by ionizing radiation.2007

    • Author(s)
      Wang L.
    • Journal Title

      Int J Radiat Biol. 83

      Pages: 433-442

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of GM-CSF receptors by real-time RT-PCR on cell lines expressing high and low affinity receptors and their relation to cytotoxic effect of chimeric protein (StxA1-GM-CSF).2007

    • Author(s)
      Roudkenar MH.
    • Journal Title

      DARU 15

      Pages: 151-155

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ラパマイシン投与による放射線耐性細胞のX線に対する増感効果2008

    • Author(s)
      桑原 義和, 森 美由紀, 中川 浩伸, 志村 勉, 福本 学
    • Organizer
      第51回日本放射線影響学会大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 放射線感受性を高感度に検出するHigh density survival assayの改良開発2008

    • Author(s)
      桑原 義和, 中川 浩伸, 森 美由紀, 志村 勉, 福本 学
    • Organizer
      第51回日本放射線影響学会大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] アラジャム ミリット 複製フォーク進行阻害により誘導されるDNA2重鎖切断の生成機構とその修復2008

    • Author(s)
      志村 勉, 桑原 義和, 福本 学
    • Organizer
      第51回日本放射線影響学会大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] DNA損傷応答遺伝子ric1の機能解析2008

    • Author(s)
      日高 征幸, 尾田 正二, 漆原 佑介, 桑原 義和, 福本 学, 三谷 啓志
    • Organizer
      第51回日本放射線影響学会大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] X線長期被ばくの生物影響:サイクリンD1の過剰発現によるG1/Sチェックポイント機構の損失2008

    • Author(s)
      志村 勉, 角田 智, 中川 浩伸, 桑原 義和, 福本 学
    • Organizer
      第51回日本放射線影響学大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] Rapamycin abrogates the phenotype of acquired radioresistance in vivo2008

    • Author(s)
      Kuwahara Y, Nakagawa H, Shimura T, Mori M, Kurihara A, Baba T, Fukumoto M
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Analysis of chromosomal loci with high frequency of loss of heterozygosity in Thorotrast-induced liver tumors2008

    • Author(s)
      Baba T, Nakagawa H, Kuwahara Y, Kurihara A, Fukumoto M.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Dose chronic exposure to ionizing radiation induce somatic mutations in the D-loop of mitochondrial DNA?2008

    • Author(s)
      Kurihara A, Kuwahara Y, Shimura T, Yamamoto Y, Fukumoto M
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Long incubation period of cancer by internal irradiation is attributed to the uneven irradiation at microscopic level2008

    • Author(s)
      Yamamoto Y, Kuwahara Y, Fukumoto M
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Establishment and characterization of a radioresistant cell line from oral squamous cell carcinoma cell line, SAS cells2008

    • Author(s)
      Nakagawa H, Kuwahara Y, Miyashita H, Mori S, Shimura T, Mori M, Baba T, Fukumoto M
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト放射線内部被ばくによる肝発がんの分子機構 : トロトラスト症から2008

    • Author(s)
      福本 学、桑原 義和
    • Organizer
      第40回日本臨床分子形態学会総会 (シンポジウム)
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝腫瘍における染色体13q31のLOH解析2007

    • Author(s)
      馬場 泰輔、桑原 義和、井上 和也、栗原 愛、福本 学
    • Organizer
      第4回東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2007-06-04
    • Related Report
      2008 Final Research Report
  • [Presentation] 放射線耐性細胞株の樹立とその特徴解析2007

    • Author(s)
      桑原 義和、馬場 泰輔、井上 和也、栗原 愛、進藤 智彦、福本 学
    • Organizer
      第4回東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2007-06-04
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝腫瘍における染色体13q31のLOH解析2007

    • Author(s)
      馬場 泰輔
    • Organizer
      第4回 東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2007-06-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] 放射線耐性細胞株の樹立とその特徴解析2007

    • Author(s)
      桑原 義和
    • Organizer
      第4回 東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2007-06-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] 放射線耐性細胞ではX線で誘発されるDNAの2本鎖切断は正確に修復される2007

    • Author(s)
      桑原 義和、馬場 泰輔、井上 和也、栗原 愛、福本 学
    • Organizer
      第50回日本放射線影響学会大会
    • Place of Presentation
      千葉
    • Related Report
      2008 Final Research Report
  • [Presentation] ミトコンドリアのD-loop領域に放射線被ばくに特異的な変異は存在するか?2007

    • Author(s)
      栗原 愛、王 ル、井上 和也、桑原 義和、山本 雄造、福本 学
    • Organizer
      第50回日本放射線影響学会大会
    • Place of Presentation
      千葉
    • Related Report
      2008 Final Research Report
  • [Presentation] 放射線耐性及び長期放射線被ばく細胞におけるゲノムワイドなメチル化領域の探索2007

    • Author(s)
      井上 和也、高橋 由明、村田 和弘、桑原 義和、志村 勉、福本 学
    • Organizer
      第50回日本放射線影響学会大会
    • Place of Presentation
      千葉
    • Related Report
      2008 Final Research Report
  • [Presentation] 分離培養したラット肝構成細胞のγ線に対する遺伝子発現変化2007

    • Author(s)
      馬場 泰輔、桑原 義和、井上 和也、栗原 愛、鈴木 実、小野 公二、福本 学
    • Organizer
      第50回日本放射線影響学会大会
    • Place of Presentation
      千葉
    • Related Report
      2008 Final Research Report
  • [Presentation] Histopathological study of rat brain exposed to X-ray microplanar beams2007

    • Author(s)
      Kurihara A, Kondoh T, Kashino G, Ono K, Shinohara K, Kuwahara Y, Fukumoto M
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Loss of heterozygosity on chromosome 8q21.2-q21.3 which is associated with Thorotrast-induced liver tumors2007

    • Author(s)
      Baba T, Kuwahara Y, Kurihara A, Fukumoto M
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of radioresistant cells established by long-term X-ray exposure2007

    • Author(s)
      Kuwahara Y, Baba T, Kurihara A, Fukumoto M
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] The mutation of ric1 induces delayed repair of DNA double strand breaks, cell death inhibition and early checkpoint release2007

    • Author(s)
      Hidaka M, Oda S, Kuwahara Y, Fukumoto M, Mitani H
    • Organizer
      13th International congress of radiation research
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Methylation of SOCS3 and p15 in carbon-ioninduced thymic lymphomas of B6C3F1 mice2007

    • Author(s)
      Shimada Y, Ebishima S, Yamaguchi Y, Kuwahara Y, Kakinuma S, Amasaki Y, Nishimura M, Imaoka T, Kobayashi Y, Sato Y
    • Organizer
      13th International congress of radiation research
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Analysis of Common Deletion (CD) and a novel deletion of mitochondrial DNA induced by ionizing radiation2007

    • Author(s)
      Kurihara A, Wang L, Kuwahara Y, Baba T, Ono K, Fukumoto M
    • Organizer
      13th International congress of radiation research
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2008 Final Research Report
  • [Presentation] Histopathological study of rat brain exposed to x-ray microplanar Beams.2007

    • Author(s)
      Kurihara A.
    • Organizer
      第66回 日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Loss of heterozygosity on chromosome 8q21.2-q21.3 which is associated with Thorotrast-induced liver tumors.2007

    • Author(s)
      Baba T.
    • Organizer
      第66回 日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of radioresistant cells established by long-term X-ray exposure.2007

    • Author(s)
      Kuwahara Y.
    • Organizer
      第66回 日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] 放射線耐性細胞ではX線で誘発されるDNAの2本鎖切断は正確に修復される2007

    • Author(s)
      桑原 義和
    • Organizer
      第50回 日本放射線影響学会大会
    • Place of Presentation
      千葉
    • Related Report
      2007 Annual Research Report
  • [Presentation] ミトコンドリアのD-loop領域に放射線被ばくに特異的な変異は存在するか?2007

    • Author(s)
      栗原愛
    • Organizer
      第50回 日本放射線影響学会大会
    • Place of Presentation
      千葉
    • Related Report
      2007 Annual Research Report
  • [Presentation] 放射線耐性及び長期放射線被ばく細胞におけるゲノムワイドなメチル化領域の探索2007

    • Author(s)
      井上和也
    • Organizer
      第50回 日本放射線影響学会大会
    • Place of Presentation
      千葉
    • Related Report
      2007 Annual Research Report
  • [Presentation] 分離培養したラット肝構成細胞のγ線に対する遺伝子発現変化2007

    • Author(s)
      馬場 泰輔
    • Organizer
      第50回 日本放射線影響学会大会
    • Place of Presentation
      千葉
    • Related Report
      2007 Annual Research Report
  • [Presentation] The mutation of ricl induces delayed repair of DNA double strand breaks, cell death inhibition and early checkpoint release.2007

    • Author(s)
      Hidaka M.
    • Organizer
      13th International congress of radiation research
    • Place of Presentation
      California, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Methylation of SOCS3 and p15 in carbon-ioninduced thymic lymphomas of B6C3F1 mice2007

    • Author(s)
      Shimada Y.
    • Organizer
      13th International congress of radiation research
    • Place of Presentation
      California, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Analysis of Common Deletion (CD) and a novel deletion of mitochondrial DNA induced by ionizing radiation.2007

    • Author(s)
      Kurihara A.
    • Organizer
      13th International congress of radiation research
    • Place of Presentation
      California, USA
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.idac.tohoku.ac.jp/dep/path/

    • Related Report
      2008 Final Research Report
  • [Remarks] 新聞掲載

    • Related Report
      2008 Final Research Report
  • [Remarks] Medical Tribune VOL. 41 NO. 3 p.35 2008

    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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