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Development of structural analysis method for protein immobilized on nano-biodevice

Research Project

Project/Area Number 19710096
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Nanomaterials/Nanobioscience
Research InstitutionShimane University

Principal Investigator

AOYAGOI Satoka  Shimane University, 生物資源科学部, 准教授 (20339683)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsタンパク質 / 分子認識 / 表面・界面物性 / ナノバイオ / 表面・界面物件 / タンバク質
Research Abstract

Immobilized-protein samples such as cytochrome b5 and lysozyme were investigated using TOF-SIMS with different primary ion sources for evaluating their orientations. Since the suggested surface side of each protein was consistent with the immobilization conditions, it is suggested that TOF-SIMS has the ability to evaluate orientation of immobilized protein molecules. Moreover, it is also indicated that the larger cluster ion source C60+ provides superior information to determine the surface part of the immobilized protein.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report

Research Products

(22 results)

All 2010 2009 2008 2007 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] クラスターイオンC60+搭載飛行時間型二次イオン質量分析法を利用した固定化リゾチウムの最表面構造評価2010

    • Author(s)
      青柳里果, 加藤信彦, 工藤正博
    • Journal Title

      医工学治療 22(1)

      Pages: 20-26

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] クラスターイオンC60+搭載飛行時間型二次イオン質量分析法を利用した固定化リゾチウムの最表面構造評価2010

    • Author(s)
      青柳里果、加藤信彦、工藤正博
    • Journal Title

      医工学治療 22(1)

      Pages: 20-26

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of pH-Dependent Staphylococcal Protein A Structural Change Using TOF-SIMS2009

    • Author(s)
      S. Aoyagi, Masahiro Higuchi, Nobuhiko Kato, Masahiro Kudo
    • Journal Title

      e-Journal of Surface Science and Nanotechnology 7

      Pages: 715-720

    • NAID

      130004439175

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of pH-Dependent Staphylococcal Protein A Structural Change Using TOF-SIMS2009

    • Author(s)
      S.Aoyagi, Masahiro Higuchi, Nobuhiko Kato, Masahiro Kudo
    • Journal Title

      e-Journal of Surface Science and Nanotechnology 7

      Pages: 715-720

    • NAID

      130004439175

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TOF-SIMS structural characterization of self-assembly monolayer of cytochrome b5 onto gold substrate2008

    • Author(s)
      S. Aoyagi, A. Rouleau, W. Boireau
    • Journal Title

      Applied Surface Science 255

      Pages: 1071-1074

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of immobilized-lysozyme by means of TOF-SIMS2008

    • Author(s)
      K. Okada, S. Aoyagi, M. Dohi, N. Kato, M. Kudo, M. Tozu, T. Miyayama, N. Sanada
    • Journal Title

      Applied Surface Science 255

      Pages: 104-1106

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] TOF-SIMS structural characterization of self-assembly monolayer of cytochrome b5 onto gold substrate2008

    • Author(s)
      S. Aoyagi, A. Rouleau and W. Boireau
    • Journal Title

      Applied Surface Science 255

      Pages: 1071-1074

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TOF-SIMS structural characterization of self-assembly monolayer of cytochrome b5 onto gold substrate2008

    • Author(s)
      S. Aoyagi, A. Rouleau W. Boireau
    • Journal Title

      Applied Surface Science (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Investigation of Molecular Surface Detection with Time-of-flight Secondary Ion Mass Spectrometry2009

    • Author(s)
      Satoka Aoyagi, Koki Namekawa, Kenichiro Yamamoto, Kiyotaka Sakai, Nobuhiko Kato, Masahiro Kudo
    • Organizer
      7th International Symposium on Atomic Level Characterizations for New Materials and Devices'09(ALC'09)
    • Place of Presentation
      ハワイ
    • Related Report
      2009 Final Research Report
  • [Presentation] C60+を用いた飛行時間型二次イオン質量分析法による固定化ポリペプチド最表面構造評価の基礎的検討2009

    • Author(s)
      青柳里果, 加藤信彦, 工藤正博
    • Organizer
      第29回表面科学学術講演会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] Evaluation of Polypeptide-immobilized indium-tin oxide electrode with C60+ primary ion source2009

    • Author(s)
      Satoka Aoyagi, Nobuhiko Kato, Masahiro Kudo
    • Organizer
      The 17th International Conference on Secondary Ion Mass Spectrometry (SIMS XVII)
    • Place of Presentation
      トロント、カナダ
    • Related Report
      2009 Final Research Report
  • [Presentation] 飛行時間型二次イオン質量分析法を利用したタンパク質構造変化評価法の開発2009

    • Author(s)
      青柳里果, 加藤信彦, 工藤正博
    • Organizer
      日本医工学治療学会第25回学術大会
    • Place of Presentation
      大阪
    • Related Report
      2009 Final Research Report
  • [Presentation] 飛行時間型二次イオン質量分析法を利用したタンパク質構造変化評価法の開発2009

    • Author(s)
      青柳里果、加藤信彦、工藤正博
    • Organizer
      日本医工学治療学会 第25回学術大会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] Evaluation of Polypeptide-immobilized indium-tin oxide electrode with C60+ primary ion source2009

    • Author(s)
      Satoka Aoyagi, Nobuhiko Kato, Masahiro Kudo
    • Organizer
      The 17th International Conference on Secondary I on Mass Spectrometry (SIMS XVII)
    • Place of Presentation
      トロント、カナダ
    • Related Report
      2009 Annual Research Report
  • [Presentation] Investigation of Molecular Surface Detection with Time-of-flight Secondary Ion Mass Spectrometry2009

    • Author(s)
      Satoka Aoyagi, Koki Namekawa, Kenichiro Yamamoto, Kiyotaka Sakai, Nobuhiko Kato, Masahiro Kudo
    • Organizer
      7th International Symposium on Atomic Level Charac terizations for New Materials and Devices'09(ALC'09)
    • Place of Presentation
      ハワイ
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development of Protein Orientation Analysis Method with TOF-SIMS2008

    • Author(s)
      Satoka Aoyagi
    • Organizer
      The 14th Symposium of Young Asian Biochemical Engineers' Community (YABEC2008)
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 飛行時間型二次イオン質量分析法を用いたプロテインAのpHによる構造変化の評価2008

    • Author(s)
      青柳里果, 樋口雅浩, 加藤信彦, 工藤正博
    • Organizer
      第28回表面科学会学術講演大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 飛行時間型二次イオン質量分析法を用いたプロテインAのpHによる構造変化の評価2008

    • Author(s)
      青柳里果, 樋口雅浩, 加藤信彦, 工藤正博
    • Organizer
      第28回表面科学会学術講演大会
    • Place of Presentation
      早稲田大学(新宿)
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of Protein Orientation Analysis Method with TOF-SIMS2008

    • Author(s)
      Satoka Aoyagi
    • Organizer
      The 14th Symposium of Young Asian Biochemical Engineers' Community (YABEC2008)
    • Place of Presentation
      芝浦工業大学(豊洲)
    • Related Report
      2008 Annual Research Report
  • [Presentation] TOF-SIMS structural characterization of self-assembly monolayer of cytochrome b5 onto gold substrate2007

    • Author(s)
      S. Aoyagi, A. Rouleau, W. Boireau
    • Organizer
      The 16th International Conference on Secondary Ion Mass Spectrometry (SIMS XVI)
    • Place of Presentation
      Kanazawa
    • Year and Date
      2007-10-29
    • Related Report
      2009 Final Research Report
  • [Presentation] TOF-SIMS structural characterization of self-assembly monolayer of cytochrome b5 onto gold substrate2007

    • Author(s)
      S. Aoyagi, A. Rouleau W. Boireau
    • Organizer
      The 16th International Conference on Secon dary Ion Mass Spectrometry (SIMS XVI)
    • Place of Presentation
      石川音楽堂(金沢)
    • Year and Date
      2007-10-29
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://bioinfoenv.shimane-u.ac.jp/aoyagi/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-03-31   Modified: 2016-04-21  

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