Site-selective anatomy of molecular interactions using dynamic force spectroscopy
| Project/Area Number |
19710109
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | University of Tsukuba |
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Principal Investigator |
TANINAKA Atsushi University of Tsukuba, 大学院・数理物質科学研究科, 助教 (80400638)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | 1分子ナノ計測 / 原子間力顕微鏡 / 動的分子間力分光法 / ポテンシャル障壁 / 機能性分子 |
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| Research Abstract |
We have demonstrated a methodology that realizes the site-selective anatomy of molecular interactions at the single-molecule level. With the combination of cross-linkers and the atomic force microscope that we developed to enable a precise analysis by dynamic force spectroscopy, direct and bridged interactions at each reaction site in a typical ligand-receptor system, sreptavidin-biotin complex, were clearly distinguished and individually analyzed for the first time, providing a greater understanding of step-by-step progress of the bonding process.
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Report
(4 results)
Research Products
(38 results)
