Dielectrophoretic patterning with different cell types and its electrochemical estimation
Project/Area Number |
19710112
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Microdevices/Nanodevices
|
Research Institution | University of Hyogo |
Principal Investigator |
TOMOYUKI Yasukawa University of Hyogo, 大学院・物質理学研究科, 准教授 (40361167)
|
Project Period (FY) |
2007 – 2008
|
Project Status |
Completed (Fiscal Year 2008)
|
Budget Amount *help |
¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
|
Keywords | 誘電泳動 / 電気化学顕微鏡 / 微粒子配列 / 細胞配列 / グルコースセンサ / 単一細胞 / 酵素センサ / 酸素選択的透過膜 |
Research Abstract |
誘電泳動を用いて迅速,簡便に細胞をパターニングできるデバイスを開発した.連続的に異種類の細胞を導入し,交流電圧の印加パターンを変化させることにより,1枚の基板上に違う種類の細胞を交互に配列することができた.マイクロ電極の先端に酸素選択的透過膜およびグルコースオキシダーゼを固定化することにより,細胞評価が可能なマイクログルコースセンサを作製できた.
|
Report
(3 results)
Research Products
(80 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Measurement of gene expression from single adherent cells and spheroids collected using fast electrical lysis2007
Author(s)
Nashimoto, Y., Takahashi, Y., Yamakawa, T., Torisawa, Y.-S., Yasukawa, T., Ito-Sasaki, T., Yokoo, M., Abe, H., Shiku, H., Kambara, H., Matsue, T.
-
Journal Title
Analytical Chemistry 79(17)
Pages: 6823-6830
Related Report
Peer Reviewed
-
[Journal Article] Microfluidic chip integrated with amperometric detector array for in situ estimating oxygen consumption characteristics of single bovine embryos2007
Author(s)
Wu, C.-C., Saito, T., Yasukawa, T., Shiku, H., Abe, H., Hoshi, H., Matsue, T.
-
Journal Title
Sensors and Actuators, B : Chemical 125(2)
Pages: 680-687
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] "Whole-cell biosensors", in Bioelectrochemistry : fundamentals, experimental techniques, and applications, Chapter 7, John Wiley & Sons Ltd.2008
Author(s)
H. Shiku, K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue
Publisher
West Sussex, UK. (Eds. P.N. Bartlett)
Related Report
-
-
-
-
-
-
-
-
-
-
-